公开(公告)号：US5780600A

公开(公告)日：1998-07-14

申请号：US448909

申请日：1995-05-24

申请人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

发明人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

IPC分类号： C12N15/09 ,  A61K35/30 ,  A61K38/00 ,  A61K38/22 ,  A61P25/00 ,  A61P25/28 ,  C07K1/14 ,  C07K1/20 ,  C07K14/435 ,  C07K14/475 ,  C07K14/48 ,  C07K14/52 ,  C07K16/22 ,  C12N1/21 ,  C12N5/10 ,  C12N15/06 ,  C12N15/12 ,  C12N15/18 ,  C12N15/31 ,  C12N15/74 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/475 ,  C07K16/22 ,  A61K38/00

摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF (SN-CNTF) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract. Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described. Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.

摘要翻译： 要求提供睫状神经营养因子（CNTF），特别是坐骨神经CNTF（SN-CNTF）。 本文所述的SN-CNTF是单一蛋白质物质，并且具有比粗提取物增加至大于25,000倍的比活性。 还提供了该SN-CNTF的氨基酸数据。 此外，还提供了使用该数据提供SN-CNTF探针和筛选cDNA和基因组文库的方法。 描述了用于生产SN-CNTF的重组DNA方法。 提供编码兔和人CNTF的核酸序列。 提供重组表达系统用于生产生物活性CNTF。

公开(公告)号：US5141856A

公开(公告)日：1992-08-25

申请号：US458564

申请日：1989-12-28

申请人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

发明人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

IPC分类号： C12N15/09 ,  A61K35/30 ,  A61K38/00 ,  A61K38/22 ,  A61P25/00 ,  A61P25/28 ,  C07K1/14 ,  C07K1/20 ,  C07K14/435 ,  C07K14/475 ,  C07K14/48 ,  C07K14/52 ,  C07K16/22 ,  C12N1/21 ,  C12N5/10 ,  C12N15/06 ,  C12N15/12 ,  C12N15/18 ,  C12N15/31 ,  C12N15/74 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/475 ,  C07K16/22 ,  A61K38/00

摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF (SN-CNTF) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract.Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described.Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.