公开(公告)号：US5780600A

公开(公告)日：1998-07-14

申请号：US448909

申请日：1995-05-24

申请人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

发明人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

IPC分类号： C12N15/09 ,  A61K35/30 ,  A61K38/00 ,  A61K38/22 ,  A61P25/00 ,  A61P25/28 ,  C07K1/14 ,  C07K1/20 ,  C07K14/435 ,  C07K14/475 ,  C07K14/48 ,  C07K14/52 ,  C07K16/22 ,  C12N1/21 ,  C12N5/10 ,  C12N15/06 ,  C12N15/12 ,  C12N15/18 ,  C12N15/31 ,  C12N15/74 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/475 ,  C07K16/22 ,  A61K38/00

摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF (SN-CNTF) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract. Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described. Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.

摘要翻译： 要求提供睫状神经营养因子（CNTF），特别是坐骨神经CNTF（SN-CNTF）。 本文所述的SN-CNTF是单一蛋白质物质，并且具有比粗提取物增加至大于25,000倍的比活性。 还提供了该SN-CNTF的氨基酸数据。 此外，还提供了使用该数据提供SN-CNTF探针和筛选cDNA和基因组文库的方法。 描述了用于生产SN-CNTF的重组DNA方法。 提供编码兔和人CNTF的核酸序列。 提供重组表达系统用于生产生物活性CNTF。

公开(公告)号：US5141856A

公开(公告)日：1992-08-25

申请号：US458564

申请日：1989-12-28

申请人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

发明人： Franklin D. Collins ,  Leu-Fen Lin ,  Drzislav Mismer ,  Christine Ko

IPC分类号： C12N15/09 ,  A61K35/30 ,  A61K38/00 ,  A61K38/22 ,  A61P25/00 ,  A61P25/28 ,  C07K1/14 ,  C07K1/20 ,  C07K14/435 ,  C07K14/475 ,  C07K14/48 ,  C07K14/52 ,  C07K16/22 ,  C12N1/21 ,  C12N5/10 ,  C12N15/06 ,  C12N15/12 ,  C12N15/18 ,  C12N15/31 ,  C12N15/74 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： C07K14/475 ,  C07K16/22 ,  A61K38/00

摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF (SN-CNTF) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract.Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described.Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.

公开(公告)号：US5459048A

公开(公告)日：1995-10-17

申请号：US153495

申请日：1993-11-17

申请人： Jerry Kuner ,  Christine Ko

发明人： Jerry Kuner ,  Christine Ko

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/30 ,  G01N33/569 ,  C07K14/00 ,  C12P21/08

CPC分类号： C07K14/30 ,  G01N33/56911 ,  A61K38/00 ,  A61K39/00

摘要： A class of polypeptides useful in an in vitro diagnosis of Mycoplasma infection in animals is disclosed. These polypeptides are also capable of inducing an immune response in swine which were previously not exposed to Mycoplasma. Recombinant DNA methods for the production of these polypeptides and certain phage vectors and DNA sequences useful in these methods are also disclosed. Methods of vaccinating animals utilizing a vaccination composition which includes these polypeptides is also disclosed.

摘要翻译： 公开了一类可用于体内诊断动物中支原体感染的多肽。 这些多肽还能够在猪中诱导免疫应答，其先前未暴露于支原体。 还公开了用于生产这些多肽的重组DNA方法和用于这些方法的某些噬菌体载体和DNA序列。 还公开了使用包括这些多肽的疫苗接种组合物接种疫苗的方法。