公开(公告)号：US07022835B1

公开(公告)日：2006-04-04

申请号：US10069974

申请日：2000-09-08

申请人： Holger Rauth ,  Richard Reinhardt ,  Eckard Nordhoff

发明人： Holger Rauth ,  Richard Reinhardt ,  Eckard Nordhoff

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C07H19/00

CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention concerns a method for binding nucleic acids to a solid phase in which a nucleic acid containing solution is contacted with a solid phase which has hydrophobic and hydrophilic groups on its surface or/and which comprises a hydrophilic, water-containing polymer matrix, in the presence of dehydrating reagents, in particular in the presence of a salt and polyethylene glycol whereby the nucleic acid is bound to the surface.

摘要翻译： 本发明涉及将核酸与固相结合的方法，其中将含核酸的溶液与其表面上具有疏水和亲水基团的固相接触或/和包含亲水性含水聚合物基质， 脱水试剂的存在，特别是在盐和聚乙二醇的存在下，由此使核酸与表面结合。