公开(公告)号：US08916175B2

公开(公告)日：2014-12-23

申请号：US11630147

申请日：2005-06-22

申请人： Jeffrey I. Cohen ,  Edward M. Cox, Jr. ,  Lesley M. Pesnicak

发明人： Jeffrey I. Cohen ,  Edward M. Cox, Jr. ,  Lesley M. Pesnicak

IPC分类号： A61K39/25 ,  A01N63/00 ,  C12N7/00 ,  C12N7/04

CPC分类号： A61K39/25 ,  A61K39/12 ,  C12N7/00 ,  C12N2710/16734 ,  C12N2710/16761 ,  C12N2710/16762

摘要： Viruses having weakened ability to establish and/or maintain latency and their use as live vaccines are described. The vaccines have one or more alterations in genes that provide continued virus replication but that inhibit latency. The vaccine materials and methods for their construction are exemplified with the varicella zoster virus. Deletion of a significant portion from both copies of the varicella zoster gene ORF63 was shown to inhibit establishment of a latent infection from a live vaccine form of the virus. Insertion of an additional ORF62 gene which is partially truncated with the ORF63 deletion inhibited establishment of latency and allowed normal growth of the virus. Other desirable viral antigen encoding sequence(s) and/or cytokine genes advantageously may replace deleted genetic material to enhance a desired immunological response. Aspects of the discovery pertain to live vaccines of other viruses, and can provide a variety of vaccines having greater safety.

摘要翻译： 描述了具有削弱建立和/或维持延迟的能力及其作为活疫苗的用途的病毒。 疫苗在提供持续病毒复制但抑制潜伏期的基因中具有一个或多个改变。 用于其构建的疫苗材料和方法以水痘带状疱疹病毒为例。 从水痘带状疱疹基因ORF63的两个拷贝中删除重要部分被证明可以抑制病毒活疫苗形式的潜伏感染的建立。 用ORF63缺失部分截断的另外的ORF62基因的插入抑制了潜伏期的建立并允许病毒的正常生长。 其他所需的病毒抗原编码序列和/或细胞因子基因有利地可以代替缺失的遗传物质以增强所需的免疫应答。 该发现的方面涉及其他病毒的活疫苗，并且可以提供具有更高安全性的多种疫苗。

公开(公告)号：US20080279886A1

公开(公告)日：2008-11-13

申请号：US11630147

申请日：2005-06-22

申请人： Jeffrey I. Cohen ,  Edward M. Cox, JR. ,  Lesley M. Pesnicak

发明人： Jeffrey I. Cohen ,  Edward M. Cox, JR. ,  Lesley M. Pesnicak

IPC分类号： A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61P37/04 ,  A61P31/12 ,  A61K39/25 ,  A61K39/205

CPC分类号： A61K39/25 ,  A61K39/12 ,  C12N7/00 ,  C12N2710/16734 ,  C12N2710/16761 ,  C12N2710/16762

摘要： Viruses having weakened ability to establish and/or maintain latency and their use as live vaccines are described. The vaccines have one or more alterations in genes that provide continued virus replication but that inhibit latency. The vaccine materials and methods for their construction are exemplified with the varicella zoster virus. Deletion of a significant portion from both copies of the varicella zoster gene ORF63 was shown to inhibit establishment of a latent infection from a live vaccine form of the virus. Insertion of an additional ORF62 gene which is partially truncated with the ORF63 deletion inhibited establishment of latency and allowed normal growth of the virus. Other desirable viral antigen encoding sequence(s) and/or cytokine genes advantageously may replace deleted genetic material to enhance a desired immunological response. Aspects of the discovery pertain to live vaccines of other viruses, and can provide a variety of vaccines having greater safety.

摘要翻译： 描述了具有削弱建立和/或维持延迟的能力及其作为活疫苗的用途的病毒。 疫苗在提供持续病毒复制但抑制潜伏期的基因中具有一个或多个改变。 用于其构建的疫苗材料和方法以水痘带状疱疹病毒为例。 从水痘带状疱疹基因ORF63的两个拷贝中删除重要部分被证明可以抑制病毒活疫苗形式的潜伏感染的建立。 用ORF63缺失部分截断的另外的ORF62基因的插入抑制了潜伏期的建立并允许病毒的正常生长。 其他所需的病毒抗原编码序列和/或细胞因子基因有利地可以代替缺失的遗传物质以增强所需的免疫应答。 该发现的方面涉及其他病毒的活疫苗，并且可以提供具有更高安全性的多种疫苗。