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公开(公告)号：US09834747B2
公开(公告)日：2017-12-05
申请号：US14449106
申请日：2014-07-31
摘要： In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.
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公开(公告)号：US10067088B2
公开(公告)日：2018-09-04
申请号：US14746269
申请日：2015-06-22
IPC分类号： G01N27/453 , G01N27/447 , C12N15/10
摘要： Gradient elution isotachophoretic apparatus, and systems for performing gradient elution isotachophoresis to separate, purify, concentrate, quantify, and/or extract charged analytes from a sample. The isotachophoretic apparatus include an electrophoretic assembly, a sampling assembly connected to the electrophoretic assembly, and/or a support structure connected to the electrophoretic assembly and/or to the sampling assembly. The system includes an isotachophoretic apparatus, and a controller communicatively coupled to the isotachophoretic apparatus. The controller includes a storage medium and a processor for executing computer readable and executable instructions.
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3.发明申请GRADIENT ELUTION ISOTACHOPHORETIC APPARATUS FOR SEPARATING, PURIFYING, CONCENTRATING, QUANTIFYING, AND/OR EXTRACTING CHARGED ANALYTES AND METHODS THEREOF 审中-公开分级，净化，浓缩，定量和/或提取电荷分析仪的梯度分析仪等离子体设备及其方法公开(公告)号：US20160139078A1
公开(公告)日：2016-05-19
申请号：US14746269
申请日：2015-06-22
IPC分类号： G01N27/447 , C12N15/10
CPC分类号： G01N27/44765 , C12N15/101 , G01N27/44721 , G01N27/4473 , G01N27/44743
摘要： Gradient elution isotachophoretic apparatus, and systems for performing gradient elution isotachophoresis to separate, purify, concentrate, quantify, and/or extract charged analytes from a sample. The isotachophoretic apparatus include an electrophoretic assembly, a sampling assembly connected to the electrophoretic assembly, and/or a support structure connected to the electrophoretic assembly and/or to the sampling assembly. The system includes an isotachophoretic apparatus, and a controller communicatively coupled to the isotachophoretic apparatus. The controller includes a storage medium and a processor for executing computer readable and executable instructions.
摘要翻译： 梯度洗脱等照相设备和用于进行梯度洗脱等速电泳以从样品中分离，纯化，浓缩，定量和/或提取带电分析物的系统。 等电位设备包括电泳组件，连接到电泳组件的采样组件和/或连接到电泳组件和/或采样组件的支撑结构。 该系统包括等离子体光学装置和通信地耦合到等离子体光刻装置的控制器。 控制器包括存储介质和用于执行计算机可读和可执行指令的处理器。
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公开(公告)号：US20150037890A1
公开(公告)日：2015-02-05
申请号：US14449106
申请日：2014-07-31
摘要： In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.
摘要翻译： 在示例性实施方案中，将核酸移植到细胞中发生在微流体室中。 核酸可以是大于100kbp的大核酸分子。 在一些情况下，微流体室仅具有一个通向流动通道的孔口。 在一些情况下，由于关闭一个或多个阀门，流过微流体室的流动暂时停止。 移植发生在室内容物被剪切力屏蔽的期间。 可以使用扩散，离心，真空通道抽吸或死端装载将细胞或缓冲液移动到腔室中。
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