METHOD FOR SEPARATING BIOLOGICAL MOLECULES AND CELLS IN SOLUTION
    7.
    发明申请
    METHOD FOR SEPARATING BIOLOGICAL MOLECULES AND CELLS IN SOLUTION 审中-公开
    分离生物分子和细胞的方法

    公开(公告)号:US20150204816A1

    公开(公告)日:2015-07-23

    申请号:US14417063

    申请日:2013-07-29

    IPC分类号: G01N27/447

    CPC分类号: G01N27/44791 G01N27/44765

    摘要: A method for separating a plurality of deformable objects, such as biological cells or biological supramolecules such as DNA, in a liquid medium by use of an electrophoretic technique combined with hydrodynamic forces. The deformable objects are introduced into a channel, having a flow axis and a cross section at right angles to the flow axis, with the minimum size of the cross section being less than or equal to 50 pm; A hydrodynamic flow is defined a in the channel together with the application of an electric field in the channel, making it possible to move the deformable objects in the channel according to the flow axis and to separate them along the flow axis. A device suitable for implementing this method. The electrolyte used for the electrophoretic separation may be a non-Newtonian fluid with viscoelastic properties.

    摘要翻译: 一种通过使用结合流体动力学的电泳技术在液体介质中分离多个可变形物体(例如生物细胞或生物超分子如DNA)的方法。 可变形物体被引入到具有流动轴线和与流动轴线成直角的横截面的通道中,其横截面的最小尺寸小于或等于50μm; 在通道中定义了流体动力学流,同时在通道中施加电场,使得可以根据流动轴线在通道中移动可变形物体并沿着流动轴线分离它们。 适合实施该方法的设备。 用于电泳分离的电解质可以是具有粘弹性的非牛顿流体。

    Control of Chemical Reactions using Isotachophoresis
    9.
    发明申请
    Control of Chemical Reactions using Isotachophoresis 有权
    使用等速电泳控制化学反应

    公开(公告)号:US20130146462A1

    公开(公告)日:2013-06-13

    申请号:US13764376

    申请日:2013-02-11

    IPC分类号: C12M1/42

    摘要: Isotachophoresis (ITP) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an ITP zone, but the resulting product of the chemical reaction is separated from this ITP zone by the ITP process. In a second aspect, one or more reactants of a chemical reaction are confined to an ITP zone, and one or more other reactants of the chemical reaction are not confined to this ITP zone. In a third aspect, ITP is employed to confine at least one reactant of a chemical reaction to an ITP zone, and at least one reactant of the chemical reaction is delivered to the ITP zone in two or more discrete doses. These aspects are especially relevant to performing polymerase chain reactions using chemical denaturants as opposed to thermal cycling.

    摘要翻译: 利用等速电泳(ITP)来控制化学反应的各个方面。 在第一方面,化学反应的至少一种反应物被限制在ITP区域,但化学反应的所得产物通过ITP方法与该ITP区域分离。 在第二方面,一种或多种化学反应的反应物被限制在ITP区域,化学反应的一种或多种其它反应物不限于该ITP区域。 在第三方面,ITP用于将至少一种化学反应的反应物限制于ITP区,并且化学反应的至少一种反应物以两个或更多个离散剂量递送至ITP区。 这些方面与使用化学变性剂而不是热循环进行聚合酶链反应特别相关。