公开(公告)号：US5821051A

公开(公告)日：1998-10-13

申请号：US840683

申请日：1997-04-29

Applicant: Elliot Androphy ,  Jason J. Chen

Inventor： Elliot Androphy ,  Jason J. Chen

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/16 ,  A61K38/17 ,  A61K38/43 ,  A61K39/12 ,  A61P31/12 ,  A61P35/00 ,  A61P43/00 ,  C07K14/47 ,  C07K19/00 ,  C12N1/21 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/15 ,  G01N33/48 ,  G01N33/569 ,  C12Q1/70

CPC classification number: C07K14/47 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00

Abstract: An assay for screening test compounds to indentify agents which modulate the binding of an E6-BP polypeptide with a papilloma virus E6 protein. The assay includes combining, as a cell-free system, an E6-binding protein or fragment thereof which binds to the E6 protein, and a test compound, and detecting the formation of a complex which includes the E6 protein and the E6-binding protein. A change in the formation of the complex in the presence of the test compound is indicative of an agent that modulates interaction between an E6 and an E6-binding protein.

Abstract translation: 筛选测试化合物以鉴定调节E6-BP多肽与乳头瘤病毒E6蛋白的结合的试剂的测定法。 该测定包括将与E6蛋白结合的E6结合蛋白或其片段和测试化合物作为无细胞系统组合，并检测包括E6蛋白和E6结合蛋白的复合物的形成 。 在测试化合物存在下复合物形成的变化指示调节E6和E6结合蛋白之间的相互作用的试剂。

公开(公告)号：US20070292408A1

公开(公告)日：2007-12-20

申请号：US11295725

申请日：2005-12-05

Applicant: Ravindra Singh ,  Natalia Singh ,  Nirmal Singh ,  Elliot Androphy

Inventor： Ravindra Singh ,  Natalia Singh ,  Nirmal Singh ,  Elliot Androphy

IPC: A61K48/00 ,  A61K38/00 ,  A61P21/00 ,  C07H21/04 ,  C12N5/06 ,  C12Q1/02

CPC classification number: C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/322 ,  C12N2320/33 ,  G01N33/6896 ,  G01N2500/04 ,  G01N2800/28

Abstract: The present invention is directed to methods and compositions capable of blocking the inhibitory effect of a newly-identified intronic inhibitory sequence element, named ISS-N1 (for “intronic splicing silencer”), located in the SMN2 gene. The compositions and methods of the instant invention include oligonucleotide reagents (e.g., oligoribonucleotides) that effectively target the SMN2 ISS-N1 site in the SMN2 pre-mRNA, thereby modulating the splicing of SMN2 pre-mRNA to include exon 7 in the processed transcript. The ISS-N1 blocking agents of the invention cause elevated expression of SMN protein, thus compensating for the loss of SMN protein expression commonly observed in subjects with spinal muscular atrophy (SMA).

Abstract translation: 本发明涉及能够阻断位于SMN2基因中的新鉴定的内含子抑制性序列元件（称为ISS-N1（对于“内含子剪接沉默子”）的抑制作用的方法和组合物。 本发明的组合物和方法包括有效靶向SMN2前mRNA中的SMN2 ISS-N1位点的寡核苷酸试剂（例如寡核糖核苷酸），由此调节SMN2前mRNA的剪接以将其包括在加工转录物中的外显子7。 本发明的ISS-N1阻断剂引起SMN蛋白的升高的表达，从而补偿在脊髓性肌萎缩（SMA）受试者中通常观察到的SMN蛋白表达的丧失。

公开(公告)号：US20050250855A1

公开(公告)日：2005-11-10

申请号：US11100709

申请日：2005-04-07

Applicant: Elliot Androphy ,  James Baleja

Inventor： Elliot Androphy ,  James Baleja

IPC: A61K31/165 ,  C12Q1/70

CPC classification number: A61K31/165 ,  G01N2333/025 ,  G01N2500/02

Abstract: Methods and compositions for treating human papillomavirus infections are described.

Abstract translation: 描述了用于治疗人乳头瘤病毒感染的方法和组合物。