公开(公告)号：US20140341843A1

公开(公告)日：2014-11-20

申请号：US14235777

申请日：2012-07-27

申请人： Thomas M. Barnes ,  Jinzhao Hou ,  Gregory Zarbis-Papastoitsis ,  Emily Belcher Schirmer ,  Gary L. McNeil ,  Kathryn Golden

发明人： Thomas M. Barnes ,  Jinzhao Hou ,  Gregory Zarbis-Papastoitsis ,  Emily Belcher Schirmer ,  Gary L. McNeil ,  Kathryn Golden

IPC分类号： C07K14/545 ,  B01D15/36

CPC分类号： C07K14/545 ,  A61K38/00 ,  B01D15/1864 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3847 ,  C07K14/54 ,  Y02A50/473

摘要： The present invention provides methods and compositions for the preparation and delivery of chimeric cytokine proteins, including cell cultures, methods of purification and purified compositions.

摘要翻译： 本发明提供了用于制备和递送嵌合细胞因子蛋白质的方法和组合物，包括细胞培养物，纯化方法和纯化的组合物。

公开(公告)号：US20110184154A1

公开(公告)日：2011-07-28

申请号：US13083538

申请日：2011-04-09

申请人： Grigorios Zarbis-Papastoitsis ,  Michael Christopher Kuczewski ,  Emily Belcher Schirmer

发明人： Grigorios Zarbis-Papastoitsis ,  Michael Christopher Kuczewski ,  Emily Belcher Schirmer

IPC分类号： C07K1/14 ,  C12N5/071 ,  C12N1/02

CPC分类号： C07K1/18

摘要： The present invention relates to a method for clarification of, and removal of host cell proteins from, a cell broth consisting essentially of viable cells, a culture medium and a secreted desired biological substance having an overall positive charge in the cell broth by contacting the cell broth with a particulate anion exchanger, allowing an adequate incubation time to result in formation of a cell pellet and a supernatant layer, separating the resulting cell pellet from the supernatant layer. The present invention further relates to a method for the recovery of a secreted desired biological substance from the cell broth by extracting the secreted desired biological substance from the supernatant layer.

摘要翻译： 本发明涉及一种用于从基本上由活细胞组成的细胞培养液澄清宿主细胞蛋白的方法，以及通过使细胞接触而在细胞培养液中具有总体正电荷的分泌的所需生物物质 肉汤与颗粒状阴离子交换剂，允许足够的孵育时间以导致细胞沉淀和上清液层的形成，将所得细胞沉淀与上清液层分离。 本发明还涉及通过从上清液层中提取分泌的所需生物物质从细胞肉汤中回收分泌的所需生物物质的方法。