公开(公告)号：US5104931A

公开(公告)日：1992-04-14

申请号：US497537

申请日：1990-03-22

Applicant: Gideon Fleminger ,  Tamar Wolf ,  Eran Hadas

Inventor： Gideon Fleminger ,  Tamar Wolf ,  Eran Hadas

IPC: A61K39/44 ,  C07K16/00 ,  C07K17/00 ,  C07K17/08 ,  C12P21/08 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547

CPC classification number: C07K17/08 ,  G01N33/54353 ,  G01N33/545 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/817

Abstract: A single-step procedure for the immobilization of antibodies, comprising reacting a purified antibody with buffered periodate in an amount sufficient to produce aldehyde groups in the presence of a polymer carrier material which has been modified with adipic acid dihydrazide is disclosed. The present invention provides dramatic improvements in activity. and yield of immobilized antibody over the prior multi-step procedure employing separate oxidation and immobilization steps.

Abstract translation: 公开了用于固定抗体的单步骤方法，其包括在已经用己二酸二酰肼修饰的聚合物载体材料存在下使纯化抗体与足够量的足量产生醛基的缓冲高碘酸盐反应。 本发明提供了显着的活性改进。 以及使用单独的氧化和固定步骤的固定化抗体相对于现有多步骤程序的产量。

公开(公告)号：US06841533B1

公开(公告)日：2005-01-11

申请号：US09644456

申请日：2000-08-24

Applicant: Vered Hornik ,  Eran Hadas

Inventor： Vered Hornik ,  Eran Hadas

IPC: A61K38/00 ,  C07K1/04 ,  C07K14/00 ,  C07K14/54 ,  C07K14/655 ,  C07K14/715 ,  A61K38/12 ,  A61K38/07 ,  C07K5/10 ,  C07K5/12

CPC classification number: C07K14/001 ,  A61K38/00 ,  C07K1/047 ,  C07K14/5412 ,  C07K14/6555 ,  C07K14/7155

Abstract: Novel peptides which are conformationally constrained backbone cyclized antagonists of IL-6, are disclosed. Methods for synthesizing the IL-6 antagonists are also disclosed. Furthermore, pharmaceutical compositions comprising IL-6 antagonists, and methods of using such compositions are disclosed.

Abstract translation: 揭示IL-6构象约束的骨架环化拮抗剂的新型肽。 还公开了合成IL-6拮抗剂的方法。 此外，公开了包含IL-6拮抗剂的药物组合物，以及使用这些组合物的方法。

公开(公告)号：US5618700A

公开(公告)日：1997-04-08

申请号：US436321

申请日：1989-11-14

Applicant: Daniela Novick ,  Michel Revel ,  Yves Mory ,  Menachem Rubinstein ,  Eran Hadas

Inventor： Daniela Novick ,  Michel Revel ,  Yves Mory ,  Menachem Rubinstein ,  Eran Hadas

IPC: A61K38/00 ,  C07K14/31 ,  C07K14/54 ,  C07K16/24 ,  C12P21/08 ,  C07K1/22 ,  C12N5/20

CPC classification number: C07K14/31 ,  C07K14/5412 ,  C07K16/248 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/705 ,  C07K2319/75

Abstract: The hybridoma 34-1 (CNCM I-813) which produces anti-human IL-6 monoclonal antibody 34-1 is disclosed. Methods of producing the monoclonal antibody and methods for affinity purification of human IL-6 using monoclonal antibody 34-1 are also described.

Abstract translation: 公开了产生抗人IL-6单克隆抗体34-1的杂交瘤34-1（CNCM I-813）。 还描述了制备单克隆抗体的方法和使用单克隆抗体34-1亲和纯化人IL-6的方法。

公开(公告)号：US4948836A

公开(公告)日：1990-08-14

申请号：US270280

申请日：1988-11-14

Applicant: Beka Solomon ,  Eran Hadas ,  Gideon Fleminger

Inventor： Beka Solomon ,  Eran Hadas ,  Gideon Fleminger

IPC: A61K39/44 ,  C07K16/00 ,  C07K17/06 ,  C07K17/08 ,  C12P21/08 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/548

CPC classification number: G01N33/54353 ,  G01N33/545 ,  G01N33/548 ,  Y10S530/815 ,  Y10S530/816

Abstract: Antibodies which are immobilized and covalently bound to a matrix polymer by means of a modification in a carbohydrate region of the antibodies; wherein the binding of the antibodies is effected by condensing at least one aldehyde group in an oxidized carbohydrate region and at least one epoxide function of an epoxy-group-containing matrix polymer, said condensation being conducted in the presence of a bifunctional reagent which has, on one end position of a spacer unit having at least three members, an amino group capable of condensing with the aldehyde group, and which has on the other end position a group which reacts covalently with the epoxide function.

Abstract translation: 通过抗体的碳水化合物区域的修饰将固定并共价结合到基质聚合物上的抗体; 其中所述抗体的结合通过将氧化碳水化合物区域中的至少一个醛基和含环氧基的基质聚合物的至少一种环氧官能团缩合来进行，所述缩合在双官能试剂的存在下进行， 在具有至少三个成员的间隔单元的一个末端位置，能够与醛基缩合的氨基，另一个位置是与环氧化物官能团共价反应的基团。