公开(公告)号：US20050267092A1

公开(公告)日：2005-12-01

申请号：US10505724

申请日：2002-02-28

申请人： Chang-Seok Lee ,  Geun-Tae Kim ,  Yong-Jin Jang ,  Eun-Jung Ryu ,  Yang-Rae Cho ,  Hyung-Yeul Joo ,  Jeong-Eun Shin ,  Sun-Hwa Lee ,  Ki-Dong Koo

发明人： Chang-Seok Lee ,  Geun-Tae Kim ,  Yong-Jin Jang ,  Eun-Jung Ryu ,  Yang-Rae Cho ,  Hyung-Yeul Joo ,  Jeong-Eun Shin ,  Sun-Hwa Lee ,  Ki-Dong Koo

IPC分类号： C07D501/36 ,  A61K31/546 ,  A61P31/04 ,  C07D501/00 ,  C07D501/24 ,  A61K31/545 ,  C07D501/14

CPC分类号： C07D501/00

摘要： The present invention relates to a novel cephalosporin compound, and pharmaceutically acceptable non-toxic salt, physiologically hydrolysable ester, hydrate, solvate or isomer thereof, to a pharmaceutical composition comprising the compound, and to a process for preparing the compound.

摘要翻译： 本发明涉及一种新颖的头孢菌素化合物及其药学上可接受的无毒盐，生理上可水解的酯，水合物，溶剂化物或异构体，其包含该化合物的药物组合物，以及制备该化合物的方法。

公开(公告)号：US06201006B1

公开(公告)日：2001-03-13

申请号：US09180675

申请日：1998-11-13

申请人： Bon Am Koo ,  Jae Ki Min ,  Woo Sang Hong ,  Eun Jung Ryu ,  Woong Hyun Nam ,  Jong Min Kim

发明人： Bon Am Koo ,  Jae Ki Min ,  Woo Sang Hong ,  Eun Jung Ryu ,  Woong Hyun Nam ,  Jong Min Kim

IPC分类号： C07D40306

CPC分类号： C07D207/08 ,  C07D401/14 ,  C07D403/06 ,  C07D403/14 ,  C07D405/06 ,  C07D405/14 ,  C07D409/06 ,  C07D409/14

摘要： The present invention relates to a novel thrombin inhibitor which is effective even when orally administered. More specifically, the present invention relates to an aromatic amidine derivative represented by formula (I) and the salts thereof, which show potent selective inhibitory activity for thrombin in which (a), R, R1, R2, R3, A, W, Y and n are defined as described in the specification.

摘要翻译： 本发明涉及即使口服给药也有效的新型凝血酶抑制剂。 更具体地说，本发明涉及由式（I）表示的芳香族脒衍生物及其盐，其中（a），R，R1，R2，R3，A，W，Y n定义如说明书中所述。

公开(公告)号：US06955879B2

公开(公告)日：2005-10-18

申请号：US10148724

申请日：2001-06-16

申请人： Si-Hyoung Lee ,  Yong-Chul Shin ,  Yeong-Joong Jeon ,  Kyung-Hwa Jung ,  Eun-Jung Ryu ,  Ko-Woon Lee

发明人： Si-Hyoung Lee ,  Yong-Chul Shin ,  Yeong-Joong Jeon ,  Kyung-Hwa Jung ,  Eun-Jung Ryu ,  Ko-Woon Lee

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12P21/06

CPC分类号： C12N15/1027

摘要： The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones. The method of the present invention can be used for in vitro recombination of homologous polynucleotides and the directed molecular evolution.

摘要翻译： 本发明涉及一种生产重组多核苷酸的方法，包括以下步骤：从两个或多个同源双链多核苷酸产生单向单链多核苷酸片段池，使用单向单链多核苷酸进行包含多循环延伸反应的聚合方法 标记的多核苷酸片段作为模板和特异性寡核苷酸作为引物以获得重组多核苷酸，并使用至少一个引物进行聚合酶链反应以扩增重组多核苷酸; 以及构建重组DNA文库的方法，包括以下步骤：将通过上述方法制备的重组多核苷酸插入载体中，并用含有重组多核苷酸的载体转化表达细胞以获得多个突变克隆。 本发明的方法可用于同源多核苷酸的体外重组和定向分子进化。