公开(公告)号：US20140087955A1

公开(公告)日：2014-03-27

申请号：US14118986

申请日：2012-05-23

申请人： Reinhold Wimberger Friedl ,  Eva Servoli

发明人： Reinhold Wimberger Friedl ,  Eva Servoli

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/6834 ,  C12Q2565/629 ,  C12Q2565/513 ,  C12Q2537/149

摘要： The present invention relates a device for the efficient binding of nucleic acids on a microarray, comprising a reaction zone comprising a microarray, and a capture zone comprising a porous membrane substrate, wherein the capture zone is capable of specifically capturing sense strands of target molecules, whereas complementary antisense strands are captured in the reaction zone, or vice versa. The invention further envisages temperature regulating units in the device allowing to bring or keep the capture at a temperature not allowing hybridizing or binding of nucleic acid(s) to the capture molecules or to a temperature suitable for nucleic acid hybridization. The invention also relates to a method of efficiently binding nucleic acids on a microarray, comprising introducing a medium containing one or more target molecules in denatured form into a capture zone of a device of the present invention, performing an interaction reaction between the target molecules and immobilized capture molecules in said capture zone, and transporting not bound target molecule strands to a reaction zone comprising a microarray, thereby allowing an interaction between the target molecule strands and immobilized capture molecules on the microarray. In a further aspect the invention relates to the use of such a device for enriching target molecules, specifically selecting a target molecules, expression analysis, comparative genomic hybridization, the detection of SNPs, or for microarray-based genomic selection-based sequencing.

摘要翻译： 本发明涉及用于在微阵列上有效结合核酸的装置，其包括包含微阵列的反应区和包含多孔膜底物的捕获区，其中所述捕获区能够特异性捕获目标分子的有义链， 而在反应区中捕获互补的反义链，反之亦然。 本发明进一步设想该装置中的温度调节单元允许将捕获带入或保持在不允许核酸与捕获分子杂交或结合的温度或适合于核酸杂交的温度。 本发明还涉及一种在微阵列上有效结合核酸的方法，包括将含有一种或多种变性形式的靶分子的培养基引入本发明的装置的捕获区，进行靶分子与 在所述捕获区中固定捕获分子，并将未结合的靶分子链输送到包含微阵列的反应区，从而允许靶分子链与微阵列上固定的捕获分子之间的相互作用。 在另一方面，本发明涉及这种装置用于富集靶分子，特异性选择靶分子，表达分析，比较基因组杂交，SNP检测或用于基于微阵列的基于基因组选择的测序的用途。

公开(公告)号：US20140017687A1

公开(公告)日：2014-01-16

申请号：US14004439

申请日：2012-03-20

申请人： Reinhold Wimberger-Friedl ,  Pieter Jan Van Der Zaag ,  Eva Servoli

发明人： Reinhold Wimberger-Friedl ,  Pieter Jan Van Der Zaag ,  Eva Servoli

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/088 ,  B01L2400/0487 ,  G01N35/08

摘要： The present invention relates to a method for performing molecular reactions in a device comprising the steps of (a) introducing one or more reagent solution(s) and an immiscible intermediate fluid into the device, wherein the device comprises a substrate, on which chemically or biochemically recognizable entities are immobilized; (b) performing molecular reactions between the immobilized chemically or biochemically recognizable entities and the reagent solution(s); or on the immobilized chemically or biochemically recognizable entities in the presence of the reagent solution(s); (c) displacing the reagent solution(s) present on the substrate by the immiscible intermediate fluid; (d) separating the immiscible intermediate fluid and the reagent solution(s); and reusing the reagent solution(s) and/or immiscible intermediate fluid for one or more repetitions of steps (a) to (e). The invention further relates to a device for performing a molecular reaction, comprising a reaction zone, reservoirs and liquid connections and a col lection and regeneration zone wherein the immiscible intermediate fluid and the reagent solution(s) are separable by gravitational separation; or a redirection and distribution module, wherein the immiscible intermediate fluid and reagent solution(s) are separated. The invention also relates to the use of an immiscible intermediate fluid for displacing a reagent solution present in a reaction zone in a microfluidic device, as well as the use of a corresponding device for performing a sequencing reaction or a nucleic acid synthesis reaction.

摘要翻译： 本发明涉及一种在器件中进行分子反应的方法，包括以下步骤：（a）将一种或多种试剂溶液和不混溶的中间体流体引入该装置，其中该装置包括基底，化学或 生化鉴定实体被固定化; （b）在固定的化学或生化学可识别的实体和试剂溶液之间进行分子反应; 或在试剂溶液存在下在固定的化学或生物化学可识别的实体上; （c）通过不混溶的中间流体置换存在于基板上的试剂溶液; （d）分离不混溶的中间体液体和试剂溶液; 并重复使用试剂溶液和/或不混溶的中间体液体进行一个或多个重复的步骤（a）至（e）。 本发明还涉及一种用于进行分子反应的装置，包括反应区，储存器和液体连接以及收集和再生区，其中不混溶的中间流体和试剂溶液可通过重力分离而分离; 或重定向和分配模块，其中不混溶的中间流体和试剂溶液被分离。 本发明还涉及不混溶中间体液体用于置换微流体装置中反应区中存在的试剂溶液的用途，以及使用相应的装置进行测序反应或核酸合成反应。