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公开(公告)号：US20140329991A9
公开(公告)日：2014-11-06
申请号：US13744305
申请日：2013-01-17
IPC分类号： C07K1/30
CPC分类号： C07K1/303
摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. A lanthanide metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for many types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
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公开(公告)号：US08835612B2
公开(公告)日：2014-09-16
申请号：US13270148
申请日：2011-10-10
申请人： Fabio Polato , Mathias Rainer , Douglas T. Gjerde , Guenther Bonn
发明人： Fabio Polato , Mathias Rainer , Douglas T. Gjerde , Guenther Bonn
CPC分类号： C07K1/32
摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
摘要翻译： 本发明提供了选择性分离磷酸化和糖基化蛋白质及其片段的方法和装置。 金属阳离子用于沉淀含有磷酸基团和/或糖基团的蛋白质或蛋白质片段。 样品制备方法可用于几种类型的生物样品，包括HeLa细胞，食物和人脑脊液。 蛋白质被分离，回收并准备好通过质谱法或其他分析方法进行分析，允许检测限降低到飞甲的水平。 该方法和装置是蛋白质分析和诊断领域的宝贵工具。
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公开(公告)号：US20140200330A1
公开(公告)日：2014-07-17
申请号：US13744305
申请日：2013-01-17
IPC分类号： C07K1/30
CPC分类号： C07K1/303
摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. A lanthanide metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for many types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
摘要翻译： 本发明提供了选择性分离磷酸化和糖基化蛋白质及其片段的方法和装置。 镧系金属阳离子用于沉淀含有磷酸基团和/或糖基团的蛋白质或蛋白质片段。 样品制备方法可用于许多类型的生物样品，包括HeLa细胞，食物和人脑脊液。 蛋白质被分离，回收并准备好通过质谱法或其他分析方法进行分析，允许检测限降低到飞甲的水平。 该方法和装置是蛋白质分析和诊断领域的宝贵工具。
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公开(公告)号：US20120088906A1
公开(公告)日：2012-04-12
申请号：US13270148
申请日：2011-10-10
申请人： Fabio Polato , Mathias Rainer , Douglas T. Gjerde , Guenther Bonn
发明人： Fabio Polato , Mathias Rainer , Douglas T. Gjerde , Guenther Bonn
CPC分类号： C07K1/32
摘要： The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
摘要翻译： 本发明提供了选择性分离磷酸化和糖基化蛋白质及其片段的方法和装置。 金属阳离子用于沉淀含有磷酸基团和/或糖基团的蛋白质或蛋白质片段。 样品制备方法可用于几种类型的生物样品，包括HeLa细胞，食物和人脑脊液。 蛋白质被分离，回收并准备好通过质谱法或其他分析方法进行分析，允许检测限降低到飞甲的水平。 该方法和装置是蛋白质分析和诊断领域的宝贵工具。
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