公开(公告)号：US20090069587A1

公开(公告)日：2009-03-12

申请号：US11853339

申请日：2007-09-11

申请人： Christopher L.G. Dayton ,  Erini Marie Rosswurm ,  Flavio da Silva Galhardo

发明人： Christopher L.G. Dayton ,  Erini Marie Rosswurm ,  Flavio da Silva Galhardo

IPC分类号： C07C51/42

CPC分类号： C12P7/6481

摘要： A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes, and wherein the reaction of step (b) continues for a duration of less than about one hour.

摘要翻译： 油组合物的脱胶方法包括以下步骤：（a）提供含有一定量磷脂的油组合物，（b）在足够的条件下，将所述油组合物与一种或多种磷脂酶A酶和一种或多种磷脂酶C酶同时接触 为了使酶与磷脂反应以产生磷脂反应产物，和（c）将磷脂反应产物与油组合物分离，分离后的剩余油组合物为脱胶油组合物，其中在步骤（b）期间， 所述一种或多种磷脂酶A酶以比不存在所述一种或多种磷脂酶C酶的速度更快的速率进行，并且其中步骤（b）的反应持续时间小于约1小时。

公开(公告)号：US09045712B2

公开(公告)日：2015-06-02

申请号：US13502105

申请日：2010-10-08

申请人： Christopher L. G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

发明人： Christopher L. G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

IPC分类号： C11B3/04 ,  C12P7/18 ,  C12N9/88 ,  C11B3/00 ,  C10L1/02 ,  C12P7/64

CPC分类号： C11B3/003 ,  C10G2300/1011 ,  C10L1/026 ,  C11B3/04 ,  C12N9/88 ,  C12P7/18 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y406/01013 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P30/20

摘要： In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.

摘要翻译： 在替代实施方案中，本发明提供磷脂酰肌醇特异性磷脂酶C（PI-PLC）酶，编码它们的核酸，与其特异性结合的抗体，以及制备和使用它们的方法。 还提供了包括使用这些磷脂酶的工业方法和产品。 在某些实施方案中，本文提供了用于在脂质基质内水合非水合磷脂（NHP）的方法。 这些方法使NHP迁移到油 - 水界面，从而允许NHP从脂质中反应和/或除去。 在某些实施方案中，提供了从植物油中除去NHP，可水合磷脂和卵磷脂以产生可用于食品生产和/或非食品应用的脱胶油或脂肪产品的方法。 在某些实施方案中，本文提供了用于NHP水合的方法，然后进行酶处理和除去各种磷脂和卵磷脂。 本文提供的方法可以在粗脱水油或水脱胶油上实施。

公开(公告)号：US08460905B2

公开(公告)日：2013-06-11

申请号：US11853339

申请日：2007-09-11

申请人： Christopher L. G. Dayton ,  Erin Marie Rosswurm ,  Flavio da Silva Galhardo

发明人： Christopher L. G. Dayton ,  Erin Marie Rosswurm ,  Flavio da Silva Galhardo

IPC分类号： C12P7/64 ,  C12P7/00 ,  C12N9/20 ,  A61K36/48 ,  C12P1/00 ,  C12N9/00 ,  C12N9/14 ,  C02F1/00 ,  C11B13/00 ,  C11B3/02

CPC分类号： C12P7/6481

摘要： A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes, and wherein the reaction of step (b) continues for a duration of less than about one hour.

摘要翻译： 油组合物的脱胶方法包括以下步骤：（a）提供含有一定量磷脂的油组合物，（b）在足够的条件下，将所述油组合物与一种或多种磷脂酶A酶和一种或多种磷脂酶C酶同时接触 为了使酶与磷脂反应以产生磷脂反应产物，和（c）将磷脂反应产物与油组合物分离，分离后的剩余油组合物为脱胶油组合物，其中在步骤（b）期间， 所述一种或多种磷脂酶A酶以比不存在所述一种或多种磷脂酶C酶的速度更快的速率进行，并且其中步骤（b）的反应持续时间小于约1小时。

公开(公告)号：US20130011887A1

公开(公告)日：2013-01-10

申请号：US13502105

申请日：2010-10-08

申请人： Christopher L.G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

发明人： Christopher L.G. Dayton ,  Flavio Da Silva Galhardo ,  Nelson Barton ,  Tim Hitchman ,  Jonathan Lyon ,  Eileen O'Donoghue ,  Mark A. Wall

IPC分类号： C11B3/02 ,  A23D7/02 ,  C09K3/00 ,  A23D7/00 ,  C12P9/00 ,  C11C1/08

CPC分类号： C11B3/003 ,  C10G2300/1011 ,  C10L1/026 ,  C11B3/04 ,  C12N9/88 ,  C12P7/18 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y406/01013 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P30/20

摘要： In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.

摘要翻译： 在替代实施方案中，本发明提供磷脂酰肌醇特异性磷脂酶C（PI-PLC）酶，编码它们的核酸，与其特异性结合的抗体，以及制备和使用它们的方法。 还提供了包括使用这些磷脂酶的工业方法和产品。 在某些实施方案中，本文提供了用于在脂质基质内水合非水合磷脂（NHP）的方法。 这些方法使NHP迁移到油 - 水界面，从而允许NHP从脂质中反应和/或除去。 在某些实施方案中，提供了从植物油中除去NHP，可水合磷脂和卵磷脂以产生可用于食品生产和/或非食品应用的脱胶油或脂肪产品的方法。 在某些实施方案中，本文提供了用于NHP水合的方法，然后进行酶处理和除去各种磷脂和卵磷脂。 本文提供的方法可以在粗脱水油或水脱胶油上实施。