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公开(公告)号:US08790901B2
公开(公告)日:2014-07-29
申请号:US13326211
申请日:2011-12-14
申请人: Subhayu Basu , Gaozhong Shen , Brett Boghigian , David Young , David Berry , Geoffrey von Maltzahn
发明人: Subhayu Basu , Gaozhong Shen , Brett Boghigian , David Young , David Berry , Geoffrey von Maltzahn
CPC分类号: A61K31/201 , C12N9/0071 , C12N9/16 , C12P7/6472 , C12Y114/19 , C12Y114/19001 , C12Y114/19006 , C12Y301/02014 , Y02A50/473
摘要: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and α-linolenic acid.
摘要翻译: 提供了包含至少18个碳原子的不饱和游离脂肪酸的生产方法。 在一些实施方案中,所述方法包括在培养基中培养工程微生物,其中所述工程化微生物包含至少一种选自酰基 - 脂质去饱和酶δ-9(EC:1.14.19.1),酰基 - 脂质去饱和酶Δ-12 (EC:1.14.19.6),酰基 - 脂肪去饱和酶Δ-15(EC:1.14.19.-)和硫酯酶(EC:3.1.2.14)。 还提供了包含这些重组酶中的至少一种的工程微生物。 所述方法和生物体可用于产生至少一种选自油酸,亚油酸和α-亚麻酸的游离脂肪酸。
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公开(公告)号:US20120116108A1
公开(公告)日:2012-05-10
申请号:US13326211
申请日:2011-12-14
申请人: Subhayu Basu , Gaozhong Shen , Brett Boghigian
发明人: Subhayu Basu , Gaozhong Shen , Brett Boghigian
CPC分类号: A61K31/201 , C12N9/0071 , C12N9/16 , C12P7/6472 , C12Y114/19 , C12Y114/19001 , C12Y114/19006 , C12Y301/02014 , Y02A50/473
摘要: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and α-linolenic acid.
摘要翻译: 提供了包含至少18个碳原子的不饱和游离脂肪酸的生产方法。 在一些实施方案中,所述方法包括在培养基中培养工程微生物,其中所述工程化微生物包含至少一种选自酰基 - 脂质去饱和酶δ-9(EC:1.14.19.1),酰基 - 脂质去饱和酶Δ-12 (EC:1.14.19.6),酰基 - 脂肪去饱和酶Δ-15(EC:1.14.19.-)和硫酯酶(EC:3.1.2.14)。 还提供了包含这些重组酶中的至少一种的工程微生物。 所述方法和生物体可用于产生至少一种选自油酸,亚油酸和α-亚麻酸的游离脂肪酸。
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