公开(公告)号：US5468643A

公开(公告)日：1995-11-21

申请号：US104522

申请日：1993-08-10

Applicant: Syang Y. Su ,  Gerald K. Shiu

Inventor： Syang Y. Su ,  Gerald K. Shiu

IPC: G01N30/02 ,  G01N30/14 ,  G01N30/16 ,  G01N30/20 ,  G01N30/24 ,  G01N30/34

CPC classification number: G01N30/16 ,  G01N2030/027 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/347 ,  G01N30/14 ,  G01N30/24 ,  Y10T436/25375

Abstract: A valving arrangement and a method are provided by which a biological sample is mixed with a reactant, such as a solvent or a precipitating agent, as it is injected through a multi-position injection valve. A multi-position switching valve is connected to the injection valve, and one or more filters attached to the switching valve remove precipitates of predetermined size from the reacted sample. The sample the flows to conventional analytical columns for liquid chromatography analysis of analytes of interest. Proteins and/or peptides are thus quickly and efficiently removed from the sample as precipitates collected in the filters. The injection valve and the switching valve are then placed in respective cooperating positions to flow a carrier fluid through the injection valve and the switching valve directly to the analysis columns, while a back-flow of a suitable surfactant is pumped through the filters to purge them of collected precipitates. This quickly purges the system and puts it in condition for a repeat of the procedure. The apparatus and method may be used both when samples are provided manually under the direct control of an operator and when an autoinjector or autosampler of known type is employed for repeated sampling.

Abstract translation: 提供了一种阀装置和方法，通过该方法将生物样品与通过多位置注射阀注射的反应物（例如溶剂或沉淀剂）混合。 多位置切换阀连接到喷射阀，并且连接到切换阀的一个或多个过滤器从反应的样品中去除预定尺寸的沉淀物。 样品流向常规分析柱，用于目标分析物的液相色谱分析。 因此，当沉淀物收集在过滤器中时，蛋白质和/或肽因此被快速有效地从样品中除去。 然后将注射阀和切换阀放置在相应的配合位置，以使载体流体通过注射阀和切换阀直接流到分析柱，同时将合适的表面活性剂的反流泵送通过过滤器以将其清除 的收集沉淀物。 这很快清除了系统，并将其置于重复该过程的条件。 当在操作者的直接控制下手动提供样品并且当使用已知类型的自动注射器或自动进样器用于重复取样时，可以使用该装置和方法。