公开(公告)号：US20110039280A1

公开(公告)日：2011-02-17

申请号：US12740087

申请日：2008-10-29

申请人： James F. Leary ,  Kinam Park ,  Ghanashyam Acharya ,  Michael Zordan

发明人： James F. Leary ,  Kinam Park ,  Ghanashyam Acharya ,  Michael Zordan

IPC分类号： G01N33/569 ,  C12M1/34

CPC分类号： G01N33/54373 ,  G01N21/253 ,  G01N21/553 ,  G01N21/6452 ,  G01N21/6458 ,  G01N21/648

摘要： A hybrid microfluidic biochip designed to perform multiplexed detection of singled- celled pathogens using a combination of SPR and epi-fluorescence imaging. The device comprises an array of gold spots, each functionalized with a capture biomolecule targeting a specific pathogen. This biosensor array is enclosed by a polydimethylsiloxane (PDMS) microfluidic flow chamber that delivers a magnetically concentrated sample to be tested. The sample is imaged by surface plasmon resonance on the bottom of the biochip, and epi- fluorescence on the top.

摘要翻译： 一种混合微流体生物芯片，设计用于使用SPR和外荧光成像的组合对单细胞病原体进行多重检测。 该装置包括金点阵列，每个金点用针对特定病原体的俘获生物分子功能化。 该生物传感器阵列由聚二甲基硅氧烷（PDMS）微流体流动室包围，其传送磁性浓缩待测样品。 样品通过生物芯片底部的表面等离子体共振进行成像，顶部呈现荧光。

公开(公告)号：US20100330702A1

公开(公告)日：2010-12-30

申请号：US12601986

申请日：2008-05-29

申请人： Cagri Savran ,  Philip Low ,  David Thompson ,  Walter Henne ,  Derek Doornweerd ,  Chun-Li Chang ,  Ghanashyam Acharya ,  David Brownholland

发明人： Cagri Savran ,  Philip Low ,  David Thompson ,  Walter Henne ,  Derek Doornweerd ,  Chun-Li Chang ,  Ghanashyam Acharya ,  David Brownholland

IPC分类号： G01N33/543 ,  G01N30/00

CPC分类号： G01N33/54386 ,  C12Q1/6804 ,  G01N21/4788 ,  G01N33/54393

摘要： Systems and methods for rapid and ultrasensitive detection of target hiomolecules in a sample are presented. The detection of biomolecules is achieved through a synergistic use of immunoseparation and diffractomctry, and the formation of antibody-biomolecule-ligand sandwich complexes that form diffraction gratings. Characteristic diffraction patterns are then produced upon illumination of the diffraction gratings with light. The diffraction patterns can he used to detect very low amounts of biomolecules present in the sample.

摘要翻译： 介绍了样品中目标高分子快速和超灵敏检测的系统和方法。 生物分子的检测通过免疫分离和衍射的协同使用以及形成衍射光栅的抗体 - 生物分子 - 配体夹心复合物的形成来实现。 然后在用光照射衍射光栅时产生特征衍射图案。 他可以使用衍射图来检测样品中存在的非常低量的生物分子。

公开(公告)号：US20090136583A1

公开(公告)日：2009-05-28

申请号：US12286147

申请日：2008-09-27

申请人： Kinam Park ,  Ghanashyam Acharya ,  Haesun Park

发明人： Kinam Park ,  Ghanashyam Acharya ,  Haesun Park

IPC分类号： A61K9/14 ,  C40B50/14 ,  B05D1/00 ,  B82B1/00 ,  B82B3/00

CPC分类号： A61K9/1682 ,  A61K9/1647 ,  A61K9/5031 ,  A61K9/5089 ,  A61K31/00 ,  Y10S977/773 ,  Y10S977/887 ,  Y10S977/89 ,  Y10S977/906

摘要： Discrete microstructures of predefined size and shape are prepared using sol-gel phase-reversible hydrogel templates. An aqueous solution of hydrogel-forming material is covered onto a microfabricated silicon wafer master template having predefined microfeatures, such as pillars. A hydrogel template is formed, usually by lowering the temperature, and the formed hydrogel template is peeled away from the silicon master template. The wells of predefined size and shape on the hydrogel template are filled with a solution or a paste of a water-insoluble polymer, and the solvent is removed to form solid structures. The formed microstructures are released from the hydrogel template by simply melting the hydrogel template in water. The microstructures are collected by centrifugation. The microstructures fabricated by this method exhibit pre-defined size and shape that exactly correspond to the microwells of the hydrogel template. The method of preparing microstructures based on hydrogel templates is simple and can easily produce large quantities of the microstructures.

摘要翻译： 使用溶胶 - 凝胶相可逆水凝胶模板制备预定尺寸和形状的离散微结构。 将水凝胶形成材料的水溶液覆盖在具有预定义的微特征（例如柱）的微制造硅晶片主模板上。 通常通过降低温度形成水凝胶模板，并将形成的水凝胶模板从硅主模板剥离。 在水凝胶模板上预定尺寸和形状的孔填充有水不溶性聚合物的溶液或糊剂，并除去溶剂以形成固体结构。 通过在水中简单地熔化水凝胶模板，将形成的微结构从水凝胶模板中释放出来。 通过离心收集微结构。 通过该方法制造的微结构显示出与水凝胶模板的微孔完全对应的预定义的尺寸和形状。 基于水凝胶模板制备微结构的方法简单，容易产生大量微结构。

公开(公告)号：US09341621B2

公开(公告)日：2016-05-17

申请号：US12601986

申请日：2008-05-29

申请人： Cagri Savran ,  Philip Low ,  David Thompson ,  Walter Henne ,  Derek Doorneweerd ,  Chun-Li Chang ,  Ghanashyam Acharya ,  David Brownholland

发明人： Cagri Savran ,  Philip Low ,  David Thompson ,  Walter Henne ,  Derek Doorneweerd ,  Chun-Li Chang ,  Ghanashyam Acharya ,  David Brownholland

IPC分类号： G01N33/53 ,  G01N33/543 ,  C12Q1/68 ,  G01N21/47

CPC分类号： G01N33/54386 ,  C12Q1/6804 ,  G01N21/4788 ,  G01N33/54393

摘要： Systems and methods for rapid and ultrasensitive detection of target biomolecules in a sample are presented. The detection of biomolecules is achieved through a synergistic use of immunoseparation and diffractometry, and the formation of antibody-biomolecule-ligand sandwich complexes that form diffraction gratings. Characteristic diffraction patterns are then produced upon illumination of the diffraction gratings with light. The diffraction patterns can be used to detect very low amounts of biomolecules present in the sample.

摘要翻译： 提出了用于样品中目标生物分子的快速和超灵敏检测的系统和方法。 通过免疫分离和衍射的协同使用以及形成衍射光栅的抗体 - 生物分子 - 配体夹心复合体的形成来实现生物分子的检测。 然后在用光照射衍射光栅时产生特征衍射图案。 衍射图可用于检测样品中存在的非常低量的生物分子。

公开(公告)号：US08951567B2

公开(公告)日：2015-02-10

申请号：US12286147

申请日：2008-09-27

申请人： Kinam Park ,  Ghanashyam Acharya ,  Haesun Park

发明人： Kinam Park ,  Ghanashyam Acharya ,  Haesun Park

IPC分类号： A61K9/14 ,  A61K9/50 ,  A61K31/00 ,  B82B1/00 ,  B82B3/00

CPC分类号： A61K9/1682 ,  A61K9/1647 ,  A61K9/5031 ,  A61K9/5089 ,  A61K31/00 ,  Y10S977/773 ,  Y10S977/887 ,  Y10S977/89 ,  Y10S977/906

摘要： Discrete microstructures of predefined size and shape are prepared using sol-gel phase-reversible hydrogel templates. An aqueous solution of hydrogel-forming material is covered onto a microfabricated silicon wafer master template having predefined microfeatures, such as pillars. A hydrogel template is formed, usually by lowering the temperature, and the formed hydrogel template is peeled away from the silicon master template. The wells of predefined size and shape on the hydrogel template are filled with a solution or a paste of a water-insoluble polymer, and the solvent is removed to form solid structures. The formed microstructures are released from the hydrogel template by simply melting the hydrogel template in water. The microstructures are collected by centrifugation. The microstructures fabricated by this method exhibit pre-defined size and shape that exactly correspond to the microwells of the hydrogel template. The method of preparing microstructures based on hydrogel templates is simple and can easily produce large quantities of the microstructures.

摘要翻译： 使用溶胶 - 凝胶相可逆水凝胶模板制备预定尺寸和形状的离散微结构。 将水凝胶形成材料的水溶液覆盖在具有预定义的微特征（例如柱）的微制造硅晶片主模板上。 通常通过降低温度形成水凝胶模板，并将形成的水凝胶模板从硅主模板剥离。 在水凝胶模板上预定尺寸和形状的孔填充有水不溶性聚合物的溶液或糊剂，并除去溶剂以形成固体结构。 通过在水中简单地熔化水凝胶模板，将形成的微结构从水凝胶模板中释放出来。 通过离心收集微结构。 通过该方法制造的微结构显示出与水凝胶模板的微孔完全对应的预定义的尺寸和形状。 基于水凝胶模板制备微结构的方法简单，容易产生大量微结构。