公开(公告)号：US4431740A

公开(公告)日：1984-02-14

申请号：US386338

申请日：1982-06-08

申请人： Graeme Bell ,  Raymond Pictet ,  Howard M. Goodman ,  William J. Rutter

发明人： Graeme Bell ,  Raymond Pictet ,  Howard M. Goodman ,  William J. Rutter

IPC分类号： A61K38/00 ,  C12N15/00 ,  C12P21/02 ,  C12N1/20 ,  C12N1/00 ,  C12P21/00

CPC分类号： C12N15/00 ,  C12P21/02 ,  A61K38/00 ,  Y10S930/30

摘要： A DNA having a base sequence coding for human proinsulin and a DNA having a base sequence coding for human pre-proinsulin have been cloned, and novel recombinant DNA transfer vectors containing said cloned DNAs have been constructed. Novel microorganisms transformed by said recombinant transfer vectors have been obtained. Certain of said transformed microorganisms have demonstrated capability to express the cloned DNA's, synthesizing a protein comprising human proinsulin and a protein-comprising human pre-proinsulin.

摘要翻译： 已经克隆了具有编码人胰岛素原的碱基序列的DNA和具有编码人前胰岛素原的碱基序列的DNA，并构建了含有所述克隆DNA的新型重组DNA转移载体。 已经获得了由所述重组转移载体转化的新型微生物。 某些所述转化的微生物已经表现出表达克隆的DNA的能力，合成包含人胰岛素原的蛋白质和含蛋白质的人类前胰岛素原。

公开(公告)号：US20060234276A1

公开(公告)日：2006-10-19

申请号：US11393643

申请日：2006-03-30

申请人： Kenneth Polonsky ,  Yukio Horikawa ,  Naohisa Oda ,  Nancy Cox ,  Seamus Sreenan ,  Yun-Ping Zhou ,  Kenichi Otani ,  Craig Hanis ,  Graeme Bell

发明人： Kenneth Polonsky ,  Yukio Horikawa ,  Naohisa Oda ,  Nancy Cox ,  Seamus Sreenan ,  Yun-Ping Zhou ,  Kenichi Otani ,  Craig Hanis ,  Graeme Bell

IPC分类号： C12Q1/68

CPC分类号： C12N9/6472 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

摘要： The present invention relates generally to the field of diabetes. More particularly, it concerns the identification of genes responsible for NIDDM1 for use in diagnostic and therapeutic applications. The present invention demonstrates that the NIDDM1 locus is, in fact, the calpain 10 gene. The invention further relates to the discovery that analysis of mutations in calpain genes and gene products can be diagnostic for type 2 diabetes. The invention also contemplates methods of treating diabetes in view of the fact that calpain mutations can cause diabetes. Further, the invention relates to novel polynucleotides of the NIDDM1 locus and polypeptides encoded by such polynucleotides.