公开(公告)号：US08673291B2

公开(公告)日：2014-03-18

申请号：US13117467

申请日：2011-05-27

Applicant: Dean M. Scholl ,  Dana M. Gebhart ,  Steven R. Williams ,  Gregory R. Govoni ,  David W. Martin, Jr.

Inventor： Dean M. Scholl ,  Dana M. Gebhart ,  Steven R. Williams ,  Gregory R. Govoni ,  David W. Martin, Jr.

IPC: C12N1/20 ,  C12N7/00 ,  C07H21/00 ,  C07K14/00

CPC classification number: C07K14/33

Abstract: This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically kill Clostridium difficile bacteria, dangerous human pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous producer cells that, unlike C. difficile, do not die in the presence of oxygen. Disclosed also is the specific gene of the isolated gene cluster that determines the killing spectrum of the R-type bacteriocin and the demonstration that the killing spectra of diffocins can be altered by engineering orf1374 of the diffocin genetic locus. This invention offers a potent bactericidal agent and a means to make it in order to kill selectively C. difficile bacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.

Abstract translation: 本公开涉及发现和分离编码特异性杀伤艰难梭菌（Clostridium difficile bacteria），危险人类病原体的R型高分子量细菌素的整个基因簇。 还公开了在无害生物细胞中生产R型细菌素的方法，其与艰难梭菌不同，不在氧的存在下死亡。 还公开了分离的基因簇的特异性基因，其确定了R型细菌素的杀伤谱，并且证明了可以通过工程化的diffocin遗传基因座的orf1374改变diffocins的杀伤谱。 本发明提供了一种有效的杀菌剂和一种使其成为在肠胃环境中选择性杀死艰难梭菌的细菌的手段，在这些细菌中它们可能会对受感染的病人或农场动物造成极大的伤害甚至死亡。

公开(公告)号：US20110293566A1

公开(公告)日：2011-12-01

申请号：US13117467

申请日：2011-05-27

Applicant: Dean M. SCHOLL ,  Dana M. Gebhart ,  Steven R. Williams ,  Gregory R. Govoni ,  David W. Martin, JR.

Inventor： Dean M. SCHOLL ,  Dana M. Gebhart ,  Steven R. Williams ,  Gregory R. Govoni ,  David W. Martin, JR.

IPC: A61K38/16 ,  C07K14/33 ,  C12P21/02 ,  A61K35/00 ,  C12N15/74 ,  C12N1/21 ,  C07H21/04 ,  A61P31/04

CPC classification number: C07K14/33

Abstract: This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically kill Clostridium difficile bacteria, dangerous human pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous producer cells that, unlike C. difficile, do not die in the presence of oxygen. Disclosed also is the specific gene of the isolated gene cluster that determines the killing spectrum of the R-type bacteriocin and the demonstration that the killing spectra of diffocins can be altered by engineering orf1374 of the diffocin genetic locus. This invention offers a potent bactericidal agent and a means to make it in order to kill selectively C. difficile bacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.

Abstract translation: 本公开涉及发现和分离编码特异性杀伤艰难梭菌（Clostridium difficile bacteria），危险人类病原体的R型高分子量细菌素的整个基因簇。 还公开了在无害生物细胞中生产R型细菌素的方法，其与艰难梭菌不同，不在氧的存在下死亡。 还公开了分离的基因簇的特异性基因，其确定了R型细菌素的杀伤谱，并且证明了可以通过工程化的diffocin遗传基因座的orf1374改变diffocins的杀伤谱。 本发明提供了一种有效的杀菌剂和一种使其成为在肠胃环境中选择性杀死艰难梭菌的细菌的手段，在这些细菌中它们可能会对受感染的病人或农场动物造成极大的伤害甚至死亡。