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公开(公告)号：US20080171376A1
公开(公告)日：2008-07-17
申请号：US11929867
申请日：2007-10-30
Applicant: Dean M. SCHOLL , Steven R. WILLIAMS
Inventor： Dean M. SCHOLL , Steven R. WILLIAMS
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如绿脓假单胞菌的R型py霉素，作为在GRAS生物体中生产它们的方法。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。
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公开(公告)号：US08673291B2
公开(公告)日：2014-03-18
申请号：US13117467
申请日：2011-05-27
Applicant: Dean M. Scholl , Dana M. Gebhart , Steven R. Williams , Gregory R. Govoni , David W. Martin, Jr.
Inventor： Dean M. Scholl , Dana M. Gebhart , Steven R. Williams , Gregory R. Govoni , David W. Martin, Jr.
CPC classification number: C07K14/33
Abstract: This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically kill Clostridium difficile bacteria, dangerous human pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous producer cells that, unlike C. difficile, do not die in the presence of oxygen. Disclosed also is the specific gene of the isolated gene cluster that determines the killing spectrum of the R-type bacteriocin and the demonstration that the killing spectra of diffocins can be altered by engineering orf1374 of the diffocin genetic locus. This invention offers a potent bactericidal agent and a means to make it in order to kill selectively C. difficile bacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.
Abstract translation: 本公开涉及发现和分离编码特异性杀伤艰难梭菌（Clostridium difficile bacteria），危险人类病原体的R型高分子量细菌素的整个基因簇。 还公开了在无害生物细胞中生产R型细菌素的方法，其与艰难梭菌不同，不在氧的存在下死亡。 还公开了分离的基因簇的特异性基因，其确定了R型细菌素的杀伤谱，并且证明了可以通过工程化的diffocin遗传基因座的orf1374改变diffocins的杀伤谱。 本发明提供了一种有效的杀菌剂和一种使其成为在肠胃环境中选择性杀死艰难梭菌的细菌的手段，在这些细菌中它们可能会对受感染的病人或农场动物造成极大的伤害甚至死亡。
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公开(公告)号：US20110293566A1
公开(公告)日：2011-12-01
申请号：US13117467
申请日：2011-05-27
Applicant: Dean M. SCHOLL , Dana M. Gebhart , Steven R. Williams , Gregory R. Govoni , David W. Martin, JR.
Inventor： Dean M. SCHOLL , Dana M. Gebhart , Steven R. Williams , Gregory R. Govoni , David W. Martin, JR.
CPC classification number: C07K14/33
Abstract: This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically kill Clostridium difficile bacteria, dangerous human pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous producer cells that, unlike C. difficile, do not die in the presence of oxygen. Disclosed also is the specific gene of the isolated gene cluster that determines the killing spectrum of the R-type bacteriocin and the demonstration that the killing spectra of diffocins can be altered by engineering orf1374 of the diffocin genetic locus. This invention offers a potent bactericidal agent and a means to make it in order to kill selectively C. difficile bacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.
Abstract translation: 本公开涉及发现和分离编码特异性杀伤艰难梭菌（Clostridium difficile bacteria），危险人类病原体的R型高分子量细菌素的整个基因簇。 还公开了在无害生物细胞中生产R型细菌素的方法，其与艰难梭菌不同，不在氧的存在下死亡。 还公开了分离的基因簇的特异性基因，其确定了R型细菌素的杀伤谱，并且证明了可以通过工程化的diffocin遗传基因座的orf1374改变diffocins的杀伤谱。 本发明提供了一种有效的杀菌剂和一种使其成为在肠胃环境中选择性杀死艰难梭菌的细菌的手段，在这些细菌中它们可能会对受感染的病人或农场动物造成极大的伤害甚至死亡。
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公开(公告)号：US08206971B2
公开(公告)日：2012-06-26
申请号：US12701431
申请日：2010-02-05
Applicant: Dean M. Scholl , Steven R. Williams
Inventor： Dean M. Scholl , Steven R. Williams
IPC: C12N1/20
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如绿脓假单胞菌的R型py霉素，作为在GRAS生物体中生产它们的方法。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。
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Patent Agency Ranking
公开(公告)号：US07732586B2
公开(公告)日：2010-06-08
申请号：US11748432
申请日：2007-05-14
Applicant: David W. Martin, Jr. , Andrew C. Jamieson , Dean M. Scholl , Steven R. Williams
Inventor： David W. Martin, Jr. , Andrew C. Jamieson , Dean M. Scholl , Steven R. Williams
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如铜绿假单胞菌的R型py虫。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。
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公开(公告)号：US07700729B2
公开(公告)日：2010-04-20
申请号：US11929867
申请日：2007-10-30
Applicant: Dean M. Scholl , Steven R. Williams
Inventor： Dean M. Scholl , Steven R. Williams
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如绿脓假单胞菌的R型py霉素，作为在GRAS生物体中生产它们的方法。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或健康因子）的方法。
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公开(公告)号：US20100261258A1
公开(公告)日：2010-10-14
申请号：US12701431
申请日：2010-02-05
Applicant: Dean M. Scholl , Steven R. Williams
Inventor： Dean M. Scholl , Steven R. Williams
IPC: C12N1/21
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如绿脓假单胞菌的R型py霉素，作为在GRAS生物体中生产它们的方法。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。
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公开(公告)号：US20080286236A1
公开(公告)日：2008-11-20
申请号：US11748437
申请日：2007-05-14
Applicant: Dana M. Gebhart , Dean M. Scholl
Inventor： Dana M. Gebhart , Dean M. Scholl
CPC classification number: G01N33/56916 , A61K38/164 , G01N2333/24 , Y02A50/406
Abstract: The disclosure relates to the targeting of Y. pestis mediated by the binding activity of tail fibers from naturally occurring R-type pyocins from Pseudomonas aeruginosa. The targeting may be mediated by a macromolecular complex such as the pyocin itself, a high molecular weight (hmw) bacteriocin modified to have the tail fiber's binding activity, or a bacteriophage modified to have the tail fiber's binding activity. Compositions comprising such complexes are described. Also disclosed are methods for the use of a complex, such as to inhibit the growth of a Yersinia species like Y. pestis, by compromising the integrity of its cytoplasmic membrane are also described. Additional methods include use of the binding activity to identify Y. pestis.
Abstract translation: 本公开涉及通过来自绿脓假单胞菌的天然存在的R型py虫的尾纤维的结合活性介导的鼠疫耶尔氏杆菌的靶向。 靶向可以由大分子复合物如pyocin本身，修饰成具有尾纤维结合活性的高分子量（hmw）细菌素或被修饰成具有尾纤维结合活性的噬菌体介导。 描述了包含这种复合物的组合物。 还公开了使用复合物的方法，例如通过损害其细胞质膜的完整性来抑制耶尔森氏菌等耶尔森氏菌属物种的生长。 其他方法包括使用结合活性来鉴定鼠疫耶氏菌。
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公开(公告)号：US20080113406A1
公开(公告)日：2008-05-15
申请号：US11748432
申请日：2007-05-14
Applicant: David W. Martin , Andrew C. Jamieson , Dean M. Scholl , Steven R. Williams
Inventor： David W. Martin , Andrew C. Jamieson , Dean M. Scholl , Steven R. Williams
IPC: C07K14/195 , C12N1/21 , C07H21/04 , C12P21/06 , C12N15/74
CPC classification number: C07K14/21 , C07K2319/35 , C07K2319/74
Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.
Abstract translation: 公开了天然存在的细菌素的修饰形式，例如铜绿假单胞菌的R型py虫。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。
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