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1.发明授权Genetic modification of superoxide dismutase to increase expression in microorganisms 失效超氧化物歧化酶的遗传修饰增加微生物的表达
公开(公告)号：US5691139A
公开(公告)日：1997-11-25
申请号：US426278
申请日：1995-04-21
CPC分类号： C12N9/0089 , C12N15/62 , C12N15/70 , C12N15/81 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。
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公开(公告)号：US5629189A
公开(公告)日：1997-05-13
申请号：US213573
申请日：1994-03-16
CPC分类号： C12N15/62 , C12N15/70 , C12N15/81 , C12N9/0089 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation. E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。 大肠杆菌菌株D1210（pSODX8）保藏在A.T.C.C. 1983年9月27日，授予登录号39453.酵母菌株2150-2-3（pC1 / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS2OR）沉积在A.T.C.C. 于一九八四年五月九日，分别获得加拿大号20708,399679及39,680。
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公开(公告)号：US5066591A
公开(公告)日：1991-11-19
申请号：US121212
申请日：1987-11-16
CPC分类号： C12N9/0089 , C12N15/62 , C12N15/70 , C12N15/81 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90
摘要： Methods and compositions are provided for the production of human copper/zinc superoxide dimutase (SOD) polypeptides in microorganisms. Bacterially produced human CuZn SOD polypeptides are provided.E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on Sept. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pCl/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and were given Accession Nos. 20708, 39679 and 39680, respectively.
摘要翻译： 提供了用于在微生物中生产人铜/锌超氧化物歧化酶（SOD）多肽的方法和组合物。 提供了细菌产生的人类CuZn SOD多肽。 大肠杆菌菌株D1210（pSODX8）保藏在A.T.C.C. 1983年9月27日，加入第39453号。酵母菌株2150-2-3（pCl / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS2OR）沉积在A.T.C.C. 1984年5月9日，分别获得了加拿大号20708,39979和39680。
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公开(公告)号：US5252476A
公开(公告)日：1993-10-12
申请号：US222352
申请日：1988-07-20
CPC分类号： C12N9/0089 , C12N15/62 , C12N15/70 , C12N15/81 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90 , Y10S435/942
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.E. coli strain D1210 (pSOD.times.8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。 大肠杆菌菌株D1210（pSODx8）保藏在A.T.C.C. 1983年9月27日，授予登录号39453.酵母菌株2150-2-3（pC1 / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS2OR）沉积在A.T.C.C. 于一九八四年五月九日，分别获得加拿大号20708,399679及39,680。
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5.发明授权DNA construct comprising a vector for expression of human cytoplasmic Cu/Zn superoxide dismutase in E. coli and method of use of same 失效包含用于在大肠杆菌中表达人细胞质Cu / Zn超氧化物歧化酶的载体的DNA构建体及其使用方法公开(公告)号：US06331421B1
公开(公告)日：2001-12-18
申请号：US08375248
申请日：1995-01-19
IPC分类号： C07H2102
CPC分类号： C12N15/62 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90 , C12N9/0089 , C12N15/70 , C12N15/81
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation. E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS20R) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。 大肠杆菌菌株D1210（pSODX8）保藏在A.T.C.C. 1983年9月27日，给予登录号39453.酵母菌株2150-2-3（pC1 / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS20R）沉积在A.T.C.C. 于一九八四年五月九日，分别获得加拿大号20708,399679及39,680。
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6.发明授权Muteins of epidermal growth factor exhibiting enhanced binding at low pH 失效表皮生长因子的突变蛋白在低pH下表现出增强的结合公开(公告)号：US06191106B1
公开(公告)日：2001-02-20
申请号：US08619032
申请日：1996-03-20
IPC分类号： C12N1518
CPC分类号： C07K14/485 , A61K38/00
摘要： EGF muteins in which the histidine at position 16 is replaced with a neutral or acidic amino acid exhibit activity at pHs lower than obtainable with wild type EGF.
摘要翻译： 其中16位组氨酸被中性或酸性氨基酸置换的EGF突变蛋白在低于用野生型EGF可获得的pH下表现出活性。
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7.发明授权Muteins of human epidermal growth factor exhibiting enhanced binding at low PH 失效在低PH下表现出增强的结合的人表皮生长因子的突变蛋白
公开(公告)号：US5547935A
公开(公告)日：1996-08-20
申请号：US284923
申请日：1994-08-02
IPC分类号： A61K38/00 , C07K14/485 , C12N15/18 , A61K38/18
CPC分类号： C07K14/485 , A61K38/00
摘要： EGF muteins in which the histidine at position 16 is replaced with a neutral or acidic amino acid exhibit activity at pHs lower than obtainable with wild type EGF.
摘要翻译： 其中16位组氨酸被中性或酸性氨基酸置换的EGF突变蛋白在低于用野生型EGF可获得的pH下表现出活性。
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8.发明授权Isolated DNA sequence comprising DNA encoding human cytoplasmic Cu-Zn superoxide dismutase 失效分离的DNA序列包含编码人胞质Cu-Zn超氧化物歧化酶的DNA
公开(公告)号：US5817794A
公开(公告)日：1998-10-06
申请号：US453448
申请日：1995-05-30
CPC分类号： C12N15/62 , C12N15/70 , C12N15/81 , C12N9/0089 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。
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公开(公告)号：US5710033A
公开(公告)日：1998-01-20
申请号：US232595
申请日：1994-04-25
CPC分类号： C12N15/62 , C12N15/70 , C12N15/81 , C12N9/0089 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/90
摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation. E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。 大肠杆菌菌株D1210（pSODX8）保藏在A.T.C.C. 1983年9月27日，授予登录号39453.酵母菌株2150-2-3（pC1 / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS2OR）沉积在A.T.C.C. 于一九八四年五月九日，分别获得加拿大号20708,399679及39,680。
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公开(公告)号：US5171680A
公开(公告)日：1992-12-15
申请号：US561442
申请日：1990-08-01
CPC分类号： C12N9/0089 , C07K14/75 , C07K14/78 , A61K38/00 , C07K2319/00 , C07K2319/33
摘要： Superoxide dismutase (SOD) analogs whose amino acid sequence includes a heterologous peptide domain such as the RGDX tetrapeptide found in fibronectin or the LGGAKQAGDV decapeptide found in fibrinogen that enables the analog to bind to a moiety that is normally present at sites of desired SOD therapy. The domain is incorporated into the SOD sequence in a manner in which it is available to interact with such moieties but does not destroy the enzymatic activity of the molecule. The inclusion of the domain enables the analog to more readily access the target tissue upon which it is to act and/or increase the corporeal half-life of the molecule.
摘要翻译： 其过氧化物歧化酶（SOD）类似物的氨基酸序列包括异源肽结构域，例如在纤连蛋白中发现的RGDX四肽或在纤维蛋白原中发现的LGGAKQAGDV十肽，其能够使模拟物结合通常存在于所需SOD治疗位点的部分。 以可用于与这些部分相互作用的方式将结构域结合到SOD序列中，但不破坏分子的酶活性。 包含该结构域使得类似物能更容易地进入其所要作用的靶组织和/或增加分子的有机半衰期。
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