公开(公告)号：US20130034585A1

公开(公告)日：2013-02-07

申请号：US13325182

申请日：2011-12-14

申请人： SHAFIQUL I. CHOWDHURY ,  HUI YONG WEI

发明人： SHAFIQUL I. CHOWDHURY ,  HUI YONG WEI

IPC分类号： A61K39/245 ,  C12N15/63 ,  A61P31/22 ,  C12N7/01

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  A61K2039/57 ,  C12N7/00 ,  C12N2710/16734 ,  C12N2710/16762 ,  C12N2710/16771

摘要： A BHV-1 mutant virus has been made that incorporates into a single virus two or more deletions in one or more of three genes—glycoprotein N, glycoprotein E and Us9. Specifically, a BHV-1 UL49.5Δ30-32 CT-null virus was made and tested. This mutant virus was then used to incorporate additional changes, e.g., the glycoprotein E cytoplasmic-tail deletion, the Us9 deletion, or both. This triple mutant BHV-1 UL49.5Δ30-32 CT-null/gE CTΔ/Us9Δ virus will be superior to the current BHV-1 mutants because the mutant virus will not be shed following reactivation, will be a DIVA based on gE CT-specific serum antibodies, and will induce better protective response by inducing higher SN titers and better cellular immune response. This new virus will have sufficient viral replication in the nasal epithelium and will be a good vaccine for protection of cattle from BHV-1. The new mutant viruses can also be used as vectors for exogenous genes.

摘要翻译： 已经制备了一种BHV-1突变病毒，其在一个或多个三个基因 - 糖蛋白N，糖蛋白E和Us9中的一个或多个中包含单个病毒两个或多个缺失。 具体来说，制造并测试了BHV-1 UL49.5＆Dgr; 30-32 CT-无效病毒。 然后将该突变型病毒用于引入额外的变化，例如糖蛋白E细胞质尾缺失，Us9缺失或两者。 这种三重突变体BHV-1 UL49.5＆Dgr; 30-32 CT-null / gE CT＆Dgr; / Us9＆Dgr; 病毒将优于目前的BHV-1突变体，因为突变型病毒在再激活后不会脱落，将是基于gE CT特异性血清抗体的DIVA，并通过诱导较高的SN滴度和更好的细胞免疫来诱导更好的保护性反应 响应。 这种新病毒将在鼻上皮中具有足够的病毒复制，并且将是用于保护牛从BHV-1的良好疫苗。 新的突变病毒也可以用作外源基因的载体。

公开(公告)号：US08877211B2

公开(公告)日：2014-11-04

申请号：US13325182

申请日：2011-12-14

申请人： Shafiqul I. Chowdhury ,  Hui Yong Wei

发明人： Shafiqul I. Chowdhury ,  Hui Yong Wei

IPC分类号： A61K39/245 ,  C12N15/63 ,  C12N7/01 ,  A61P31/22 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  A61K2039/57 ,  C12N7/00 ,  C12N2710/16734 ,  C12N2710/16762 ,  C12N2710/16771

摘要： A BHV-1 mutant virus has been made that incorporates into a single virus two or more deletions in one or more of three genes—glycoprotein N, glycoprotein E and Us9. Specifically, a BHV-1 UL49.5Δ30-32 CT-null virus was made and tested. This mutant virus was then used to incorporate additional changes, e.g., the glycoprotein E cytoplasmic-tail deletion, the Us9 deletion, or both. This triple mutant BHV-1 UL49.5Δ30-32 CT-null/gE CTΔ/Us9Δ virus will be superior to the current BHV-1 mutants because the mutant virus will not be shed following reactivation, will be a DIVA based on gE CT-specific serum antibodies, and will induce better protective response by inducing higher SN titers and better cellular immune response. This new virus will have sufficient viral replication in the nasal epithelium and will be a good vaccine for protection of cattle from BHV-1. The new mutant viruses can also be used as vectors for exogenous genes.

摘要翻译： 已经制备了一种BHV-1突变病毒，其在一个或多个三个基因 - 糖蛋白N，糖蛋白E和Us9中的一个或多个中包含单个病毒两个或多个缺失。 具体来说，制造并测试了BHV-1 UL49.5＆Dgr; 30-32 CT-无效病毒。 然后将该突变型病毒用于引入额外的变化，例如糖蛋白E细胞质尾缺失，Us9缺失或两者。 这种三重突变体BHV-1 UL49.5＆Dgr; 30-32 CT-null / gE CT＆Dgr; / Us9＆Dgr; 病毒将优于目前的BHV-1突变体，因为突变型病毒在再激活后不会脱落，将是基于gE CT特异性血清抗体的DIVA，并通过诱导较高的SN滴度和更好的细胞免疫来诱导更好的保护性反应 响应。 这种新病毒将在鼻上皮中具有足够的病毒复制，并且将是用于保护牛从BHV-1的良好疫苗。 新的突变病毒也可以用作外源基因的载体。