-
公开(公告)号：US11083787B2
公开(公告)日：2021-08-10
申请号：US15780900
申请日：2016-12-02
IPC分类号： A61K39/245 , A61K39/39 , C07K14/005 , A61K39/12 , A61K39/00
摘要： The application describes recombinant BoHV-1 triple mutant viruses that express protective antigens of other bovine respiratory viruses associated with Bovine respiratory disease complex (BRDC).
-
公开(公告)号：US08877211B2
公开(公告)日：2014-11-04
申请号：US13325182
申请日：2011-12-14
CPC分类号： A61K39/245 , A61K39/12 , A61K2039/5254 , A61K2039/552 , A61K2039/57 , C12N7/00 , C12N2710/16734 , C12N2710/16762 , C12N2710/16771
摘要： A BHV-1 mutant virus has been made that incorporates into a single virus two or more deletions in one or more of three genes—glycoprotein N, glycoprotein E and Us9. Specifically, a BHV-1 UL49.5Δ30-32 CT-null virus was made and tested. This mutant virus was then used to incorporate additional changes, e.g., the glycoprotein E cytoplasmic-tail deletion, the Us9 deletion, or both. This triple mutant BHV-1 UL49.5Δ30-32 CT-null/gE CTΔ/Us9Δ virus will be superior to the current BHV-1 mutants because the mutant virus will not be shed following reactivation, will be a DIVA based on gE CT-specific serum antibodies, and will induce better protective response by inducing higher SN titers and better cellular immune response. This new virus will have sufficient viral replication in the nasal epithelium and will be a good vaccine for protection of cattle from BHV-1. The new mutant viruses can also be used as vectors for exogenous genes.
摘要翻译： 已经制备了一种BHV-1突变病毒，其在一个或多个三个基因 - 糖蛋白N，糖蛋白E和Us9中的一个或多个中包含单个病毒两个或多个缺失。 具体来说，制造并测试了BHV-1 UL49.5＆Dgr; 30-32 CT-无效病毒。 然后将该突变型病毒用于引入额外的变化，例如糖蛋白E细胞质尾缺失，Us9缺失或两者。 这种三重突变体BHV-1 UL49.5＆Dgr; 30-32 CT-null / gE CT＆Dgr; / Us9＆Dgr; 病毒将优于目前的BHV-1突变体，因为突变型病毒在再激活后不会脱落，将是基于gE CT特异性血清抗体的DIVA，并通过诱导较高的SN滴度和更好的细胞免疫来诱导更好的保护性反应 响应。 这种新病毒将在鼻上皮中具有足够的病毒复制，并且将是用于保护牛从BHV-1的良好疫苗。 新的突变病毒也可以用作外源基因的载体。
-
公开(公告)号：US20240084325A1
公开(公告)日：2024-03-14
申请号：US18268553
申请日：2021-12-21
IPC分类号： C12N15/86 , A61K39/12 , A61P31/14 , C07K14/005
CPC分类号： C12N15/86 , A61K39/12 , A61P31/14 , C07K14/005 , A61K2039/552 , C12N2710/16734 , C12N2710/16743 , C12N2710/16762
摘要： The invention relates to a Quadruple Gene Deleted Mutant Bovine Herpesvirus Type 1 (BHV-1 QMV) engineered to express protective antigens derived from viruses associated with infection in livestock. The recombinant vector includes a deletion of a cytoplasmic tail of envelope glycoprotein gE (gE-CT), a truncation of glycoprotein gG, a deletion of envelope protein UL49.5 amino acid residues 30-32, and a deletion of UL49.5 cytoplasmic tail amino acid residues 80-96. The truncation of glycoprotein gG comprises a deletion of amino-terminal amino acid residues 1-67. The recombinant vector can include at least two heterologous antigens inserted therein. Included are methods for creating recombinant vectors, mutant viruses, and vaccines for preventing or reducing symptoms associated with viral infection in livestock, in particular bovine respiratory viral infection
-
公开(公告)号：US06284251B1
公开(公告)日：2001-09-04
申请号：US09240173
申请日：1999-01-29
IPC分类号： A61K39265
CPC分类号： C07K14/005 , A61K2039/5254 , A61K2039/5256 , A61K2039/543 , C12N7/00 , C12N15/86 , C12N2710/16722 , C12N2710/16743 , C12N2710/16761
摘要： A safe live recombinant virus as well as a vaccine is produced by the deletion of a portion of the native glycoprotein E (gE) coding region of bovine herpesvirus 1 (BHV-1) followed by the insertion of a plasmid including a foreign functional &bgr;-gal at the gE locus. The deletion of gE gene stably attenuates the virus and serves as an immunological marker differentiating vaccinated animals from infected animals. Moreover, production of &bgr;-gal allows easy assessment of gE&Dgr;3.1IBR&bgr; virus replication in vaccinated animals and serves as a phenotypic marker which distinguishes it from wild type virus replication in infected animals.
摘要翻译： 通过删除牛疱疹病毒1（BHV-1）的天然糖蛋白E（gE）编码区的一部分，然后插入含有外源功能β-谷氨酸的质粒，产生安全的活重组病毒以及疫苗， gal在gE位点。 gE基因的缺失可以稳定地减弱病毒，并且作为将感染动物免受感染的动物区分开的免疫标记。 此外，β-gal的生产允许容易地评估接种疫苗的动物中的gEDELTA3.1IBRbeta病毒复制，并用作将其与感染动物中的野生型病毒复制区分开的表型标记。
-
公开(公告)号：US06221360B1
公开(公告)日：2001-04-24
申请号：US08607323
申请日：1996-02-26
IPC分类号： A61K39265
CPC分类号： C12N7/00 , A61K2039/5254 , A61K2039/5256 , A61K2039/543 , C07K14/005 , C12N2710/16061 , C12N2710/16722
摘要： The disclosed invention is directed to (1) recombinant bovine herpesvirus genes having foreign genes inserted therein, (2) infectious recombinant bovine herpesviruses carrying these recombinant genes, (3) methods of producing these recombinant bovine herpesviruses, (4) methods of immunizing animals against diseases caused by bovine herpesviruses using these recombinant bovine herpesviruses as vaccines, and (5) methods of detecting infection of an animal by these recombinant bovine herpesviruses. In its preferred form, the invention is directed to the construction of an infectious recombinant bovine herpesvirus type 1 having a functional &bgr;-galactosidase gene inserted in and thereby inactivating the thymidine kinase gene (e.g., Gal-TK); infection of an animal with the resultant avirulent vaccine virus is detected by assaying the animal's respiratory secretions for the presence of virus having &bgr;-galactosidase activity in host cells.
摘要翻译： 所公开的发明涉及（1）在其中插入有外源基因的重组牛疱疹病毒基因，（2）携带这些重组基因的感染性重组牛疱疹病毒，（3）产生这些重组牛疱疹病毒的方法，（4） 使用这些重组牛疱疹病毒作为疫苗的牛疱疹病毒引起的疾病，以及（5）通过这些重组牛疱疹病毒检测动物感染的方法。 在其优选形式中，本发明涉及一种具有插入功能性β-半乳糖苷酶基因并由此使胸苷激酶基因（例如Gal-TK）失活的感染性重组牛疱疹病毒1型的构建; 通过测定动物的呼吸道分泌物以检测宿主细胞中具有β-半乳糖苷酶活性的病毒的存在来检测具有所得无毒力疫苗病毒的动物的感染。
-
公开(公告)号：US20220062407A1
公开(公告)日：2022-03-03
申请号：US17372044
申请日：2021-07-09
IPC分类号： A61K39/245 , A61K39/39 , C07K14/005 , A61K39/12
摘要： The application describes recombinant BoHV-1 triple mutant viruses that express protective antigens of other bovine respiratory viruses associated with Bovine respiratory disease complex (BRDC).
-
公开(公告)号：US20180353596A1
公开(公告)日：2018-12-13
申请号：US15780900
申请日：2016-12-02
IPC分类号： A61K39/245 , A61K39/39
CPC分类号： A61K39/245 , A61K39/12 , A61K39/39 , A61K2039/5254 , A61K2039/543 , A61K2039/552 , A61K2039/70 , C07K14/005 , C12N2710/16734 , C12N2710/16743 , C12N2710/16771 , C12N2760/18534 , C12N2770/24334
摘要： The application describes recombinant BoHV-1 triple mutant viruses that express protective antigens of other bovine respiratory viruses associated with Bovine respiratory disease complex (BRDC).
-
公开(公告)号：US20130034585A1
公开(公告)日：2013-02-07
申请号：US13325182
申请日：2011-12-14
IPC分类号： A61K39/245 , C12N15/63 , A61P31/22 , C12N7/01
CPC分类号： A61K39/245 , A61K39/12 , A61K2039/5254 , A61K2039/552 , A61K2039/57 , C12N7/00 , C12N2710/16734 , C12N2710/16762 , C12N2710/16771
摘要： A BHV-1 mutant virus has been made that incorporates into a single virus two or more deletions in one or more of three genes—glycoprotein N, glycoprotein E and Us9. Specifically, a BHV-1 UL49.5Δ30-32 CT-null virus was made and tested. This mutant virus was then used to incorporate additional changes, e.g., the glycoprotein E cytoplasmic-tail deletion, the Us9 deletion, or both. This triple mutant BHV-1 UL49.5Δ30-32 CT-null/gE CTΔ/Us9Δ virus will be superior to the current BHV-1 mutants because the mutant virus will not be shed following reactivation, will be a DIVA based on gE CT-specific serum antibodies, and will induce better protective response by inducing higher SN titers and better cellular immune response. This new virus will have sufficient viral replication in the nasal epithelium and will be a good vaccine for protection of cattle from BHV-1. The new mutant viruses can also be used as vectors for exogenous genes.
摘要翻译： 已经制备了一种BHV-1突变病毒，其在一个或多个三个基因 - 糖蛋白N，糖蛋白E和Us9中的一个或多个中包含单个病毒两个或多个缺失。 具体来说，制造并测试了BHV-1 UL49.5＆Dgr; 30-32 CT-无效病毒。 然后将该突变型病毒用于引入额外的变化，例如糖蛋白E细胞质尾缺失，Us9缺失或两者。 这种三重突变体BHV-1 UL49.5＆Dgr; 30-32 CT-null / gE CT＆Dgr; / Us9＆Dgr; 病毒将优于目前的BHV-1突变体，因为突变型病毒在再激活后不会脱落，将是基于gE CT特异性血清抗体的DIVA，并通过诱导较高的SN滴度和更好的细胞免疫来诱导更好的保护性反应 响应。 这种新病毒将在鼻上皮中具有足够的病毒复制，并且将是用于保护牛从BHV-1的良好疫苗。 新的突变病毒也可以用作外源基因的载体。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.015 秒)
