公开(公告)号：US10401604B2

公开(公告)日：2019-09-03

申请号：US15511580

申请日：2015-09-24

Applicant: The United States of America, as Represented by the Secretary, Dept. of Health and Human Services ,  Hari Shroff ,  Yicong Wu ,  Sara Abrahamsson

Inventor： Hari Shroff ,  Yicong Wu ,  Sara Abrahamsson

IPC: G01N1/00 ,  G02B21/00 ,  G02B21/16 ,  G01N21/64 ,  G02B21/18 ,  G02B27/00 ,  G02B21/10 ,  G02B27/42

Abstract: Embodiments of a resolution enhancement technique for a light sheet microscopy system having a three objective lens arrangement in which one objective lens illuminates a sample and the second and third objective lenses collect the fluorescence emissions emitted by the sample are disclosed. The second objective lens focuses a first portion of the fluorescence emissions for detection by a second detection component, while the third objective lens focuses a second portion of the fluorescence emissions through a diffractive or refractive optic component for detection by a first detector component. A processor combines the images resulting from the first and second portions of the fluorescence emissions for generating composite images with increased axial and lateral resolution.

公开(公告)号：US09696534B2

公开(公告)日：2017-07-04

申请号：US14380392

申请日：2013-02-22

Applicant: THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES OFFICE OF TECHNOLOGY TRANSFER, NATIONAL INTITUTES OF HEALTH ,  Hari Shroff ,  Andrew York

Inventor： Hari Shroff ,  Andrew York

IPC: G01N21/64 ,  G02B21/00

CPC classification number: G02B21/0072 ,  G01N21/6458 ,  G01N2021/6478 ,  G01N2201/0633 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/004 ,  G02B21/0076

Abstract: A multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample is disclosed. The multi-focal SIM system performs a focusing, scaling and summing operation on each multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

公开(公告)号：US09316824B2

公开(公告)日：2016-04-19

申请号：US14003380

申请日：2012-03-02

Applicant: Hari Shroff ,  Yicong Wu ,  Gary Rondeau

Inventor： Hari Shroff ,  Yicong Wu ,  Gary Rondeau

IPC: G02B21/06 ,  G02B21/00

CPC classification number: G02B21/06 ,  G02B21/0004

Abstract: A module for engagement with a conventional microscope to provide selective plane illumination microscopy is disclosed. The module is coupled to the translational base of the microscope and defines a mounting having a mount body in which an excitation objective having a first longitudinal axis is engaged to one portion of the mount body and a detection objective having a second longitudinal axis is engaged to another portion of the mount body such that the first longitudinal axis is in perpendicular geometric relation with the second longitudinal axis.

Abstract translation: 公开了一种用于与常规显微镜接合以提供选择性平面照明显微镜的模块。 模块耦合到显微镜的平移基座并且限定具有安装体的安装件，其中具有第一纵向轴线的激励物镜接合到安装体的一部分，并且具有第二纵向轴线的检测物体接合到 安装体的另一部分使得第一纵向轴线与第二纵向轴线垂直几何关系。

公开(公告)号：US20190324240A1

公开(公告)日：2019-10-24

申请号：US16475508

申请日：2018-06-06

Applicant: Hari Shroff ,  Andrew York

Inventor： Hari Shroff ,  Andrew York

IPC: G02B21/00 ,  G01N21/64

Abstract: Various embodiments (300, 400, 500) for a multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample are disclosed. The embodiments (300, 400, 500) of the multi-focal SIM system perform a focusing, scaling and summing operation on each generated multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

公开(公告)号：US20140126046A1

公开(公告)日：2014-05-08

申请号：US14003380

申请日：2012-03-02

Applicant: Hari Shroff ,  Yicong Wu ,  Gary Rondeau

Inventor： Hari Shroff ,  Yicong Wu ,  Gary Rondeau

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B21/0004

Abstract: A module for engagement with a conventional microscope to provide selective plane illumination microscopy is disclosed. The module is coupled to the translational base of the microscope and defines a mounting having a mount body in which an excitation objective having a first longitudinal axis is engaged to one portion of the mount body and a detection objective having a second longitudinal axis is engaged to another portion of the mount body such that the first longitudinal axis is in perpendicular geometric relation with the second longitudinal axis.

Abstract translation: 公开了一种用于与常规显微镜接合以提供选择性平面照明显微镜的模块。 模块耦合到显微镜的平移基座并且限定具有安装体的安装件，其中具有第一纵向轴线的激励物镜接合到安装体的一部分，并且具有第二纵向轴线的检测物体接合到 安装体的另一部分使得第一纵向轴线与第二纵向轴线垂直几何关系。