公开(公告)号：US20100196955A1

公开(公告)日：2010-08-05

申请号：US12531037

申请日：2008-03-14

Applicant: Harmit Vora ,  James McIntire ,  Pawan Kumar ,  Chaitan Khosla

Inventor： Harmit Vora ,  James McIntire ,  Pawan Kumar ,  Chaitan Khosla

IPC: C12P21/00

CPC classification number: C12N9/641

Abstract: Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product.

Abstract translation: 提供了以冻干形式生产克数至千克量的前EP-B2（EP-B2的酶原形式）的方法。 这些方法包括可扩展发酵，重折叠和纯化过程，该方法可以与冻干结合以产生稳定的产物。

公开(公告)号：US08148105B2

公开(公告)日：2012-04-03

申请号：US12531037

申请日：2008-03-14

Applicant: Harmit Vora ,  James McIntire ,  Pawan Kumar ,  Chaitan Khosla

Inventor： Harmit Vora ,  James McIntire ,  Pawan Kumar ,  Chaitan Khosla

IPC: C12N15/09 ,  C12N15/29 ,  C12N15/57 ,  C12N9/50 ,  C07K1/14 ,  C07K1/22

CPC classification number: C12N9/641

Abstract: Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product.

Abstract translation: 提供了以冻干形式生产克数至千克量的前EP-B2（EP-B2的酶原形式）的方法。 这些方法包括可扩展发酵，重折叠和纯化过程，该方法可以与冻干结合以产生稳定的产物。