PLASMID FOR EXPRESSING THIOREDOXIN FUSION PROTEIN AND METHOD FOR PRODUCING TARGET PROTEIN USING SAME
    1.
    发明申请
    PLASMID FOR EXPRESSING THIOREDOXIN FUSION PROTEIN AND METHOD FOR PRODUCING TARGET PROTEIN USING SAME 审中-公开
    用于表达三羟甲基纤维素融合蛋白的PLASMID和使用其生产目标蛋白的方法

    公开(公告)号:US20100184146A1

    公开(公告)日:2010-07-22

    申请号:US12546367

    申请日:2009-08-24

    CPC分类号: C12N15/70 C07K2319/35

    摘要: The present invention provides a plasmid for expressing a thioredoxin fusion protein comprising thioredoxin as a fusion partner, an E. coli cell transformed with the plasmid, and an efficient method for producing a target protein using the E. coli cell. The method according to the present invention can easily remove thioredoxin from thioredoxin fusion protein, so that can produce a target protein of interest in pure and large quantities in the field of medicine and bioengineering.

    摘要翻译: 本发明提供用于表达包含硫氧还蛋白作为融合配偶体的硫氧还蛋白融合蛋白的质粒,用质粒转化的大肠杆菌细胞,以及使用大肠杆菌细胞产生靶蛋白的有效方法。 根据本发明的方法可以容易地从硫氧还蛋白融合蛋白中除去硫氧还蛋白,从而可以在医药和生物工程领域中纯大量生产目的蛋白质。