公开(公告)号：US20100184146A1

公开(公告)日：2010-07-22

申请号：US12546367

申请日：2009-08-24

申请人： Hee-Chul Ahn ,  Hyung Jun Ahn ,  Dong Yun Shin ,  Jong Soo Lim ,  Dae-Ro Ahn

发明人： Hee-Chul Ahn ,  Hyung Jun Ahn ,  Dong Yun Shin ,  Jong Soo Lim ,  Dae-Ro Ahn

IPC分类号： C12P21/02 ,  C12N15/70 ,  C12N1/21 ,  C12P21/04

CPC分类号： C12N15/70 ,  C07K2319/35

摘要： The present invention provides a plasmid for expressing a thioredoxin fusion protein comprising thioredoxin as a fusion partner, an E. coli cell transformed with the plasmid, and an efficient method for producing a target protein using the E. coli cell. The method according to the present invention can easily remove thioredoxin from thioredoxin fusion protein, so that can produce a target protein of interest in pure and large quantities in the field of medicine and bioengineering.

摘要翻译： 本发明提供用于表达包含硫氧还蛋白作为融合配偶体的硫氧还蛋白融合蛋白的质粒，用质粒转化的大肠杆菌细胞，以及使用大肠杆菌细胞产生靶蛋白的有效方法。 根据本发明的方法可以容易地从硫氧还蛋白融合蛋白中除去硫氧还蛋白，从而可以在医药和生物工程领域中纯大量生产目的蛋白质。

公开(公告)号：US08541378B2

公开(公告)日：2013-09-24

申请号：US12792186

申请日：2010-06-02

申请人： Hyung Jun Ahn ,  Ick Chan Kwon ,  Kuiwon Choi ,  Kwangmyeng Kim ,  Inchan Youn ,  Sehoon Kim

发明人： Hyung Jun Ahn ,  Ick Chan Kwon ,  Kuiwon Choi ,  Kwangmyeng Kim ,  Inchan Youn ,  Sehoon Kim

IPC分类号： A61K38/10

CPC分类号： C07K14/76 ,  A61K47/643 ,  C07K14/765 ,  C07K2319/20

摘要： The present invention relates to recombinant albumins fused with poly-cysteine peptide and methods for preparing the same, more precisely recombinant albumins in which cysteines that can be used for drug binding are amplified at N-terminal and C-terminal of the albumin and methods for preparing the same. The recombinant albumin of the present invention demonstrates improved albumin-drug conjugation efficiency when it is used for drug delivery system, indicating that it can effectively deliver a large amount of drug to a target tissue. At the same time, the recombinant albumin of the present invention can be used as an excellent drug deliverer with reduced side effects, compared with the conventional albumin carriers, by regulating the amount of drug conjugated to each unit of albumin by regulating the number of cysteine fused thereto. In addition, the recombinant albumin of the present invention can be used for the screening of a novel drug and for the non-invasive real-time diagnosis and treatment of disease by combining with a fluorescent material or a contrast agent for molecular imaging.

摘要翻译： 本发明涉及与多聚半胱氨酸肽融合的重组白蛋白及其制备方法，更准确地说，重组白蛋白，其中可用于药物结合的半胱氨酸在白蛋白的N末端和C末端扩增， 准备一样 本发明的重组白蛋白在用于药物递送系统时表现出改善的白蛋白 - 药物共轭效率，表明其可有效地将大量药物递送至靶组织。 同时，与常规白蛋白载体相比，本发明的重组白蛋白可以用作优异的药物递送剂，通过调节半胱氨酸数目调节与每单位白蛋白结合的药物量 融合。 此外，本发明的重组白蛋白可以用于筛选新药和用于分子成像的荧光材料或造影剂的非侵入性实时诊断和治疗疾病。