公开(公告)号：US20130203112A1

公开(公告)日：2013-08-08

申请号：US13877628

申请日：2011-10-07

申请人： Hee-Sung Park ,  Dieter Soll

发明人： Hee-Sung Park ,  Dieter Soll

IPC分类号： C12P21/00

CPC分类号： C12N15/67 ,  C07K14/245 ,  C07K14/435 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02 ,  C12Y601/01027

摘要： Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNASep) and methods of use in site specific incorporation of phosphoserine into a protein or polypeptide are described. Typically, SepRS preferentially aminoacylates tRNASep with O-phosphoserine and the tRNASep recognizes at least one codon such as a stop codon. Due to the negative charge of the phosphoserine, Sept-tRNASep does not bind elongation factor Tu (EF-Tu). However, mutant EF-Sep proteins are disclosed that bind Sep-tRNASep and protect Sep-tRNASep from deacylation. In a preferred embodiment the nucleic acids are on vectors and are expressed in cells such as bacterial cells, archeaebacterial cells, and eukaryotic cells. Proteins or polypeptides containing phosphoserine produced by the methods described herein can be used for a variety of applications such as research, antibody production, protein array manufacture and development of cell-based screens for new drug discovery.

公开(公告)号：US20060029115A1

公开(公告)日：2006-02-09

申请号：US11197347

申请日：2005-08-05

申请人： Hee-sung Park ,  Chong-sam Chung ,  Kwang Kim ,  Jin-won Lee

发明人： Hee-sung Park ,  Chong-sam Chung ,  Kwang Kim ,  Jin-won Lee

IPC分类号： H01S3/04

CPC分类号： H01S5/024 ,  H01S5/02268 ,  H01S5/02292

摘要： An optical head with a heat sink. The optical head includes an optical bench; a laser diode combined with the optical bench; a first heat sink combined with a lower surface of the optical bench; and a second heat sink combined with an upper surface of the optical bench. A heat-dissipation structure of the laser diode with high output may be improved.

摘要翻译： 带散热片的光学头。 光头包括光学台; 激光二极管与光学平台组合; 与光台的下表面结合的第一散热器; 以及与光学台的上表面结合的第二散热器。 可以提高具有高输出的激光二极管的散热结构。

公开(公告)号：US20050150016A1

公开(公告)日：2005-07-07

申请号：US10500447

申请日：2002-11-19

申请人： Hee-Sung Park

发明人： Hee-Sung Park

IPC分类号： C07K14/415 ,  C12N9/72 ,  C12N9/80 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N9/6459 ,  C12N9/80 ,  C12N15/8205 ,  C12N15/8257 ,  C12Y304/21069

摘要： The invention relates to method for producing a recombinant protein using pollen. More specifically, the invention relates to method for producing a recombinant protein by introducing a recombinant gene into pollen using Agrobacterium tumefaciens via vacuum infiltration and then inducing pollen tube growth, and it makes possible to scale-up recombinant protein within short period of time and low production costs.

摘要翻译： 本发明涉及使用花粉生产重组蛋白的方法。 更具体地说，本发明涉及通过真空浸渗法使用根癌土壤杆菌将重组基因引入花粉，然后诱导花粉管生长而制备重组蛋白质的方法，并且可以在短时间内将重组蛋白放大 生产成本。

公开(公告)号：US20100047882A1

公开(公告)日：2010-02-25

申请号：US12516278

申请日：2007-11-27

申请人： Gyu-Nam Cho ,  Won-Seop Choi ,  Yong-Bum Seo ,  Seung-Woo Han ,  Yoo-Shin Kim ,  Moung-Ki Shin ,  Hee-Sung Park ,  Soon-Won Hong

发明人： Gyu-Nam Cho ,  Won-Seop Choi ,  Yong-Bum Seo ,  Seung-Woo Han ,  Yoo-Shin Kim ,  Moung-Ki Shin ,  Hee-Sung Park ,  Soon-Won Hong

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12N1/02

摘要： Provided are a method of recovering L-threonine from the fermentation broth of an L-threonine producing microorganism, comprising: separating microbial bodies from the L-threonine containing fermentation broth obtained by culturing an L-threonine producing microorganism and filtering the separated fermentation broth to obtain a filtrate; concentrating the filtrate; and reacting the concentrated filtrate with a nonsolvent to obtain crystalline L-threonine, crystalline L-threonine recovered by the method, and a feed additive containing the crystalline L-threonine recovered by the method.

摘要翻译： 本发明提供从L-苏氨酸生成微生物的发酵液中回收L-苏氨酸的方法，其特征在于：将通过培养L-苏氨酸生成微生物而得到的含有L-苏氨酸的发酵液的微生物体分离，并将分离的发酵液过滤 得到滤液; 浓缩滤液; 并使浓缩的滤液与非溶剂反应，得到结晶L-苏氨酸，通过该方法回收的结晶L-苏氨酸和含有通过该方法回收的结晶L-苏氨酸的饲料添加剂。

公开(公告)号：US07388746B2

公开(公告)日：2008-06-17

申请号：US11519072

申请日：2006-09-12

申请人： Hee-sung Park ,  Jae-young Chang ,  Jong-in Jo ,  Han-kook Choi ,  Jin-wook Yoon

发明人： Hee-sung Park ,  Jae-young Chang ,  Jong-in Jo ,  Han-kook Choi ,  Jin-wook Yoon

IPC分类号： H05K7/20 ,  H01L23/34

CPC分类号： H01L23/4006 ,  H01L23/467 ,  H01L23/473 ,  H01L2023/4056 ,  H01L2023/4062 ,  H01L2224/73253

摘要： A heatsink assembly having a simple structure for coupling a heatsink and an electronic chip is provided with a heatsink which contacts an electronic chip mounted on a substrate and absorbs heat generated from the electronic chip; and a pressing member which is coupled to the substrate and comprises: a pressing cover for pressing the heatsink so that the heatsink can be secured to the electronic chip; and tension parts which extend from the pressing cover and exert a pressing force onto the pressing cover, when being coupled to the substrate.

摘要翻译： 具有用于耦合散热器和电子芯片的简单结构的散热器组件设置有与安装在基板上的电子芯片接触并吸收从电子芯片产生的热量的散热器; 以及联接到所述基板的按压构件，包括：用于按压所述散热器的按压盖，使得所述散热器能够固定到所述电子芯片; 以及张紧部，其从所述按压盖延伸并且当与所述基板结合时向所述按压盖施加按压力。

公开(公告)号：US20070068652A1

公开(公告)日：2007-03-29

申请号：US11520012

申请日：2006-09-13

申请人： Hee-sung Park ,  Sun-soo Kim

发明人： Hee-sung Park ,  Sun-soo Kim

IPC分类号： H05K7/20

CPC分类号： H01L23/473 ,  H01L2924/0002 ,  H01L2924/00

摘要： Provided is a heatsink, including a cooling zone to contact an object to be frozen and having microchannels formed therein to define a plurality of fluid pathways, an inlet through which a working fluid enters, a distributor, interposed between the inlet and the cooling zone, to uniformly distribute the working fluid introduced through the inlet to the microchannels, an outlet through which the working fluid, passing through the cooling zone, exits, and a collector, interposed between the cooling zone and the outlet, to collect the working fluid passing through the microchannels.

摘要翻译： 本发明提供一种散热器，其包括与被冷冻物接触的冷却区，并具有形成在其中的微通道，以限定多个流体路径，工作流体进入的入口，介于入口与冷却区之间的分配器， 将通过入口引入的工作流体均匀地分配到微通道，通过冷却区的工作流体离开的出口和介于冷却区和出口之间的收集器，以收集通过的工作流体 微通道。

公开(公告)号：US20060194087A1

公开(公告)日：2006-08-31

申请号：US11325415

申请日：2006-01-05

申请人： Hee-Sung Park ,  Kwang Kim

发明人： Hee-Sung Park ,  Kwang Kim

IPC分类号： H01M8/04 ,  H01M8/12 ,  H01M8/24

CPC分类号： H01M8/04007 ,  H01M8/04201 ,  H01M8/1011 ,  H01M2250/30 ,  Y02B90/18 ,  Y02E60/523

摘要： A cooling system using fuel of a fuel cell as a refrigerant comprises a fuel cartridge for storing fuel for a fuel cell stack that generates electricity and heat by an electrochemical reaction. The system further comprises a heat collection unit for lowering a temperature of an electronic device by allowing the fuel to absorb the heat generated from the electronic device, and a heat dissipating unit for dissipating the heat absorbed from the electronic device to an external side. The fuel stored in the fuel cartridge circulates between the heat collection unit, the heat dissipating unit, and the fuel cartridge, thereby lowering the temperature of the electronic device.

摘要翻译： 使用燃料电池的燃料作为制冷剂的冷却系统包括用于储存通过电化学反应产生电和热的燃料电池堆的燃料的燃料盒。 该系统还包括：通过允许燃料吸收从电子设备产生的热量来降低电子设备的温度的热采集单元，以及用于将从电子设备吸收的热量散发到外部的散热单元。 存储在燃料盒中的燃料在集热单元，散热单元和燃料盒之间循环，从而降低电子设备的温度。

公开(公告)号：US09090928B2

公开(公告)日：2015-07-28

申请号：US13877628

申请日：2011-10-07

申请人： Hee-Sung Park ,  Dieter Soll

发明人： Hee-Sung Park ,  Dieter Soll

IPC分类号： C12P21/06 ,  C12P21/00 ,  C12N15/67 ,  C12P21/02

CPC分类号： C12N15/67 ,  C07K14/245 ,  C07K14/435 ,  C12N9/93 ,  C12P21/00 ,  C12P21/02 ,  C12Y601/01027

摘要： Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNASep) and methods of use in site specific incorporation of phosphoserine into a protein or polypeptide are described. Typically, SepRS preferentially aminoacylates tRNASep with O-phosphoserine and the tRNASep recognizes at least one codon such as a stop codon. Due to the negative charge of the phosphoserine, Sept-tRNASep does not bind elongation factor Tu (EF-Tu). However, mutant EF-Sep proteins are disclosed that bind Sep-tRNASep and protect Sep-tRNASep from deacylation. In a preferred embodiment the nucleic acids are on vectors and are expressed in cells such as bacterial cells, archeaebacterial cells, and eukaryotic cells. Proteins or polypeptides containing phosphoserine produced by the methods described herein can be used for a variety of applications such as research, antibody production, protein array manufacture and development of cell-based screens for new drug discovery.

摘要翻译： 描述编码突变体延伸因子蛋白（EF-Sep），磷酰基-tRNA合成酶（SepRS）和磷酸丝氨酰-tRNA（tRNASep）的核酸以及磷酸丝氨酸位点特异性掺入蛋白质或多肽的方法。 通常，SepRS优先用O-磷酸丝氨酸氨基酰化tRNASep，tRNASep识别至少一个密码子，如终止密码子。 由于磷酸丝氨酸的负电荷，Sept-tRNASep不结合延伸因子Tu（EF-Tu）。 然而，公开了结合Sep-tRNASep并保护Sep-tRNASep免于脱酰作用的突变EF-Sep蛋白。 在优选的实施方案中，核酸在载体上并在细胞如细菌细胞，原核细菌细胞和真核细胞中表达。 通过本文描述的方法产生的含有磷酸丝氨酸的蛋白质或多肽可用于各种应用，例如研究，抗体生产，蛋白质阵列制造和用于新药物发现的基于细胞的屏幕的开发。

公开(公告)号：US07441591B2

公开(公告)日：2008-10-28

申请号：US11520012

申请日：2006-09-13

申请人： Hee-sung Park ,  Sun-soo Kim

发明人： Hee-sung Park ,  Sun-soo Kim

IPC分类号： F28F7/00 ,  F28F13/08 ,  H05K7/20

CPC分类号： H01L23/473 ,  H01L2924/0002 ,  H01L2924/00

摘要： Provided is a heatsink, including a cooling zone to contact an object to be frozen and having microchannels formed therein to define a plurality of fluid pathways, an inlet through which a working fluid enters, a distributor, interposed between the inlet and the cooling zone, to uniformly distribute the working fluid introduced through the inlet to the microchannels, an outlet through which the working fluid, passing through the cooling zone, exits, and a collector, interposed between the cooling zone and the outlet, to collect the working fluid passing through the microchannels.

摘要翻译： 本发明提供一种散热器，其包括与被冷冻物接触的冷却区，并具有形成在其中的微通道，以限定多个流体路径，工作流体进入的入口，介于入口与冷却区之间的分配器， 将通过入口引入的工作流体均匀地分配到微通道，通过冷却区的工作流体离开的出口和介于冷却区和出口之间的收集器，以收集通过的工作流体 微通道。

公开(公告)号：US20070070603A1

公开(公告)日：2007-03-29

申请号：US11519072

申请日：2006-09-12

申请人： Hee-sung Park ,  Jae-young Chang ,  Jong-in Jo ,  Han-kook Choi ,  Jin-wook Yoon

发明人： Hee-sung Park ,  Jae-young Chang ,  Jong-in Jo ,  Han-kook Choi ,  Jin-wook Yoon

IPC分类号： H05K7/20

CPC分类号： H01L23/4006 ,  H01L23/467 ,  H01L23/473 ,  H01L2023/4056 ,  H01L2023/4062 ,  H01L2224/73253

摘要： A heatsink assembly having a simple structure for coupling a heatsink and an electronic chip is provided with a heatsink which contacts an electronic chip mounted on a substrate and absorbs heat generated from the electronic chip; and a pressing member which is coupled to the substrate and comprises: a pressing cover for pressing the heatsink so that the heatsink can be secured to the electronic chip; and tension parts which extend from the pressing cover and exert a pressing force onto the pressing cover, when being coupled to the substrate.

摘要翻译： 具有用于耦合散热器和电子芯片的简单结构的散热器组件设置有与安装在基板上的电子芯片接触并吸收从电子芯片产生的热量的散热器; 以及联接到所述基板的按压构件，包括：用于按压所述散热器的按压盖，使得所述散热器能够固定到所述电子芯片; 以及张紧部，其从所述按压盖延伸并且当与所述基板结合时向所述按压盖施加按压力。