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公开(公告)号：US5766912A
公开(公告)日：1998-06-16
申请号：US230170
申请日：1994-04-20
IPC分类号： A01H5/02 , C12N1/15 , C12N9/20 , C12N9/28 , C12N9/30 , C12N9/64 , C12N15/52 , C12N15/67 , C12N15/80 , C12P1/02 , C12P21/02
CPC分类号： C12N9/20 , A01H5/02 , C12N15/67 , C12N15/80 , C07K2319/02
摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
摘要翻译： 公开了在米曲霉中表达蛋白质产物的方法。 该方法包括用包含编码促进基因表达功能的DNA序列的载体系统，用于选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。 该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。 这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。 还公开了用于在曲霉中表达蛋白质的有效启动子。 优选的启动子是TAKA-淀粉酶启动子或其功能部分。 还提供了用于生产重组Humicola脂肪酶的方法。 来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。
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公开(公告)号：US5945328A
公开(公告)日：1999-08-31
申请号：US956267
申请日：1997-10-22
CPC分类号： C12N9/6427
摘要： Trypsin (trypsinogen) may be produced in a filamentous fungus by transforming a filamentous fungus with a vector comprising a DNA sequence encoding protrypsin or a derivative thereof N-terminally fused to a DNA sequence encoding a signal peptide, culturing the transformed filamentous fungus in a suitable culture medium to produce trypsinogen and recovering trypsinogen and/or trypsin from the medium.
摘要翻译： 胰蛋白酶（胰蛋白酶原）可以在丝状真菌中通过用包含编码早胰蛋白酶的DNA序列的载体或其衍生物转化丝状真菌，N-末端融合到编码信号肽的DNA序列，培养转化的丝状真菌合适 培养基以产生胰蛋白酶原并从培养基中回收胰蛋白酶原和/或胰蛋白酶。
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公开(公告)号：US5874558A
公开(公告)日：1999-02-23
申请号：US650086
申请日：1996-05-17
IPC分类号： A01H5/02 , C12N9/20 , C12N9/28 , C12N9/30 , C12N9/64 , C12N15/52 , C12N15/67 , C12N15/80 , C07H21/04
CPC分类号： C12N9/2417 , A01H5/02 , C12N15/52 , C12N15/67 , C12N15/80 , C12N9/20 , C12N9/242 , C12N9/6481 , C12N9/6483 , C12Y304/23004 , C07K2319/02
摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
摘要翻译： 公开了在米曲霉中表达蛋白质产物的方法。 该方法包括用包含编码促进基因表达的功能的DNA序列的载体系统，选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。 该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。 这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。 还公开了用于在曲霉中表达蛋白质的有效启动子。 优选的启动子是TAKA-淀粉酶启动子或其功能部分。 还提供了用于生产重组Humicola脂肪酶的方法。 来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。
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公开(公告)号：US5965384A
公开(公告)日：1999-10-12
申请号：US463172
申请日：1995-06-05
IPC分类号： A01H5/02 , C12N1/15 , C12N9/20 , C12N9/28 , C12N9/30 , C12N9/64 , C12N15/52 , C12N15/67 , C12N15/80 , C12P21/02
CPC分类号： C12N9/20 , A01H5/02 , C12N15/67 , C12N15/80 , C07K2319/02
摘要： A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.
摘要翻译： 公开了在米曲霉中表达蛋白质产物的方法。 该方法包括用包含编码促进基因表达功能的DNA序列的载体系统，用于选择转化体的合适标记物和编码所需蛋白质产物的DNA序列来转化米曲霉。 该方法能够在米曲霉中工业生产许多不同的多肽和蛋白质。 这些产品的实例是凝乳酶或凝乳酶原和其他雷诺丝，蛋白酶，脂肪酶和淀粉酶。 还公开了用于在曲霉中表达蛋白质的有效启动子。 优选的启动子是TAKA-淀粉酶启动子或其功能部分。 还提供了用于生产重组Humicola脂肪酶的方法。 来自米曲霉的重组Humicola脂肪酶与天然脂肪酶的不同之处在于具有更大的糖基化并具有改善的热稳定性。
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公开(公告)号：US5686593A
公开(公告)日：1997-11-11
申请号：US479939
申请日：1995-06-07
IPC分类号： C12N1/19 , C12N9/42 , C12N15/09 , C12R1/645 , C12R1/77 , C07H21/02 , A61K38/47 , C07H21/04 , G01N33/53
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to cellulose- or hemicellulose-degrading enzymes which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises the following amino acid sequence. ##STR1## or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.
摘要翻译： 本发明涉及可从除木霉属或Phanerochaete之外的真菌衍生的纤维素或半纤维素降解酶，其包含与里氏木霉纤维素酶的末端A区同源的碳水化合物结合结构域，所述碳水化合物结合结构域包含以下氨基 酸序列。 将酶与不溶性纤维素或半纤维素基质结合起来。
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公开(公告)号：US5763254A
公开(公告)日：1998-06-09
申请号：US483432
申请日：1995-06-07
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to isolated and purified cellulose- or hemicellulose-degrading enzymes, comprising a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, wherein the carbohydrate binding domain has the following core sequence: ##STR1## wherein the enzyme is endogenous to a strain of a fungus other than Trichoderma or Phanerochaete.
摘要翻译： 本发明涉及分离和纯化的纤维素或半纤维素降解酶，其包含催化活性结构域，碳水化合物结合结构域和可操作地连接催化活性结构域和碳水化合物结合结构域的连接B区，其中碳水化合物结合结构域具有 以下核心序列：
其中所述酶对除木霉属或Phanerochaete之外的真菌菌株是内源的。
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