公开(公告)号：US06338943B1

公开(公告)日：2002-01-15

申请号：US09402707

申请日：1999-10-08

Applicant: Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

Inventor： Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

IPC: C12Q168

CPC classification number: C07H23/00 ,  C07H21/00 ,  Y10S436/80 ,  Y10T436/143333

Abstract: The present invention provides improved methods for labeling nucleotides. The method of the invention for labeling nucleotides comprises the steps of: reacting a reactive moiety of a linker, which linker is a platinum compound having a stabilizing bridge and two reactive moieties, with an electron donating moiety of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label; reacting the other reactive moiety of said linker with a nucleotide. A major advantage of the invention is that all nucleotides can be labeled by the method of the invention, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.

Abstract translation: 本发明提供了用于标记核苷酸的改进方法。 用于标记核苷酸的本发明的方法包括以下步骤：使接头的反应性部分（该接头是具有稳定桥和两个反应性部分的铂化合物）与间隔基的给电子部分反应，该间隔基包括链 在链中具有至少四个原子和至少一个杂原子，该间隔基进一步包含链一端的所述给电子部分和链的另一端的反应性部分; 使所述间隔物的反应性部分与标记反应; 使所述接头的另一反应性部分与核苷酸反应。 本发明的主要优点是所有的核苷酸都可以通过本发明的方法进行标记，而到目前为止，标记的连接主要限于一个或某些核苷酸。

公开(公告)号：US06797818B2

公开(公告)日：2004-09-28

申请号：US10047874

申请日：2002-01-14

Applicant: Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

Inventor： Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

IPC: C07H2102

CPC classification number: C07H23/00 ,  C07H21/00 ,  Y10S436/80 ,  Y10T436/143333

Abstract: The present invention provides a labeled nucleotide having formula: where X is an aliphatic diamine, and the labeling moiety comprises a label and a spacer, where the spacer is coupled at one end to a platinum atom and at the other end to the label, which spacer comprises a chain having at least four atoms. The invention further provides a method for labeling a nucleotide, kits for labeling a nucleotide, and kits containing labeled nucleotides.

Abstract translation: 本发明提供了一种标记的核苷酸，其具有式：其中X是脂族二胺，标记部分包括标记和间隔基，其中间隔物一端与铂原子结合，另一端与标记偶联， 间隔基包含具有至少四个原子的链。 本发明还提供了用于标记核苷酸的方法，用于标记核苷酸的试剂盒和含有标记核苷酸的试剂盒。

公开(公告)号：US07217813B2

公开(公告)日：2007-05-15

申请号：US10950985

申请日：2004-09-27

Applicant: Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

Inventor： Hendrick Jan Houthoff ,  Jan Reedijk ,  Tinka Jelsma ,  Robert Jochem Heetebrij ,  Herman Hendricus Volkers

IPC: C07H21/00 ,  C07H19/04 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C07H23/00 ,  C07H21/00 ,  Y10S436/80 ,  Y10T436/143333

Abstract: The present invention provides a labeled nucleoside having formula: where X represents an aliphatic diamine, and the labeling moiety comprises a label and a spacer, where the spacer is coupled at one end to a platinum atom and at the other end to the label, which spacer comprises a chain having at least four carbon atoms and at least one oxygen atom in the chain. The invention further provides a method for labeling a nucleoside, kits for labeling a nucleoside, and kits for producing a labeled nucleic acid.

Abstract translation: 本发明提供了具有下式的标记核苷：其中X表示脂族二胺，标记部分包含标记和间隔基，其中间隔物一端与铂原子结合，另一端与标记偶联， 间隔基包含链中具有至少四个碳原子和至少一个氧原子的链。 本发明还提供了用于标记核苷的方法，用于标记核苷的试剂盒和用于产生标记的核酸的试剂盒。