Transfection reagent for non-adherent suspension cells
    1.
    发明申请
    Transfection reagent for non-adherent suspension cells 审中-公开
    非粘附悬浮细胞的转染试剂

    公开(公告)号:US20060228406A1

    公开(公告)日:2006-10-12

    申请号:US11384178

    申请日:2006-03-17

    IPC分类号: A61K48/00 A61K9/127

    摘要: The present invention discloses liposomal transfection reagents for delivery of macromolecules and other compounds into cells, particularly non-adherent suspension cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.

    摘要翻译: 本发明公开了用于将大分子和其它化合物递送到细胞,特别是非粘附悬浮细胞中的脂质体转染试剂。 它们对细胞的DNA依赖性转化特别有用。 还公开了其制备和用作细胞内递送剂的方法。

    Methods and compositions for introducing biopolymers into cells
    2.
    发明申请
    Methods and compositions for introducing biopolymers into cells 审中-公开
    将生物聚合物引入细胞的方法和组合物

    公开(公告)号:US20060257858A1

    公开(公告)日:2006-11-16

    申请号:US11396287

    申请日:2006-03-31

    IPC分类号: C12Q1/70 C12Q1/68

    摘要: The invention relates to methods for introducing target biopolymers (e.g., nucleic acid molecules, proteins, etc.) and other compounds (e.g., non-polymeric organic molecules, such as steroids) into cells (e.g., eukaryotic cells). The invention further relates to compositions comprising target biopolymers and other compounds. In certain aspects, the invention relates to the deposition of target biopolymer-containing mixtures onto surfaces optionally followed by contacting these target biopolymer-containing mixtures with cells (e.g., eukaryotic cells). Additionally, when the target biopolymers are nucleic acid molecules, expression of these nucleic acid molecules by cells which are contacted with the target biopolymers may be monitored or detected.

    摘要翻译: 本发明涉及将靶生物聚合物(例如,核酸分子,蛋白质等)和其它化合物(例如非聚合有机分子,例如类固醇)引入细胞(例如真核细胞)的方法。 本发明还涉及包含靶生物聚合物和其它化合物的组合物。 在某些方面,本发明涉及将含目标生物聚合物的混合物沉积在表面上,任选地随后将这些含目标生物聚合物的混合物与细胞(例如真核细胞)接触。 另外,当目标生物聚合物是核酸分子时,可以监测或检测这些与目标生物聚合物接触的细胞的这些核酸分子的表达。