公开(公告)号：US20180326383A1

公开(公告)日：2018-11-15

申请号：US15969972

申请日：2018-05-03

申请人： MAK ENGINEERING CORPORATION ,  RIGAKU KOBO, LLC

发明人： Isao Kotani ,  Nobuyuki Nakayama

IPC分类号： B01J8/10 ,  B01J19/00 ,  B01F13/08

CPC分类号： B01J8/10 ,  B01F13/0818 ,  B01F13/1022 ,  B01J19/0066 ,  B01J2219/00004 ,  B01J2219/00479 ,  B01J2219/0074

摘要： A compact desktop continuous stirred tank reactor easily used on a magnetic stirrer is provided. A desktop continuous stirred tank reactor used on a magnetic stirrer includes a plurality of containers, each of the plurality of containers having a bottom and a shape capable of containing a stir bar, the plurality of containers being configured as a single unit member. The plurality of containers is arranged on the circumference of a circle of rotation of a pair of magnets of the magnetic stirrer or inside the circumference, and adjacent containers communicate through communication holes.

公开(公告)号：US20180245133A1

公开(公告)日：2018-08-30

申请号：US15965155

申请日：2018-04-27

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Matthew T. O'Keefe ,  Pamela K. Foreman

IPC分类号： C12Q1/686 ,  C12Q1/6883

CPC分类号： C12Q1/686 ,  B01F13/0059 ,  B01F13/0071 ,  B01F13/0084 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0431 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/50857 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/10 ,  B01L2300/165 ,  B01L2400/0688 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  C40B40/06 ,  C40B60/14 ,  H01J49/0418 ,  Y02P20/582

摘要： Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

公开(公告)号：US20180172595A1

公开(公告)日：2018-06-21

申请号：US15898283

申请日：2018-02-16

申请人： Meso Scale Technologies, LLC.

发明人： Eli N. Glezer ,  Jonathan K. Leland ,  Mark A. Billadeau ,  Joseph M. Leginus ,  Bandele Jeffrey-Coker ,  Jeff D. Debad ,  Koustubh A. Phalnikar ,  Sriram Jambunathan

IPC分类号： G01N21/76 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/00 ,  G01N27/30 ,  G01N33/543

CPC分类号： G01N21/76 ,  B01J19/0046 ,  B01J2219/00653 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/5029 ,  B01L9/527 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/049 ,  B01L2400/0688 ,  B33Y80/00 ,  C12Q1/001 ,  C40B40/10 ,  G01N27/30 ,  G01N33/5438 ,  G01N2458/30 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

摘要： Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.

公开(公告)号：US20180120310A1

公开(公告)日：2018-05-03

申请号：US15852939

申请日：2017-12-22

申请人： Aushon Biosystems, Inc.

发明人： Peter HONKANEN ,  Christine A. BURNS

IPC分类号： G01N33/543 ,  B01L3/00

CPC分类号： G01N33/54393 ,  B01J2219/00315 ,  B01J2219/00385 ,  B01J2219/00576 ,  B01J2219/00693 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5085 ,  B01L2200/148 ,  B01L2300/0636 ,  B01L2300/0829 ,  G01N33/54366

摘要： An apparatus and a method are disclosed for printing in-well calibration features onto assay substrates. An apparatus includes a testing substrate; a plurality of capture compound features in a well of the testing substrate; a calibration feature on one of the capture compound features in the well of the testing substrate, where the calibration feature has a known concentration of a compound that is capable of binding to the capture compound; and at least one additional capture compound feature in the same well of the testing substrate, where the at least one additional capture compound feature does not have a calibration feature printed onto the at least one additional capture compound feature. Methods for using the same are disclosed.

公开(公告)号：US20180051243A1

公开(公告)日：2018-02-22

申请号：US15557706

申请日：2016-03-11

申请人： The Trustees of the University of Pennsylvania

发明人： Michael HOGAN ,  Orkan M. TELHAN ,  Karen HOGAN

IPC分类号： C12M1/26 ,  C12M1/36 ,  C12M1/42 ,  C12M1/34 ,  C12M1/00 ,  C12M3/00

CPC分类号： C12M33/04 ,  B01J19/0046 ,  B01J2219/0029 ,  B01J2219/00326 ,  B01J2219/00353 ,  B01J2219/00376 ,  B01J2219/0054 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/0074 ,  C12M23/44 ,  C12M23/50 ,  C12M31/04 ,  C12M35/02 ,  C12M41/34 ,  C12M41/46 ,  C12M41/48 ,  C12M47/06 ,  C12M47/12

摘要： A fluid transfer system includes a transfer carousel capable of rotational and/or translational movement; at least one holding vessel (e.g. syringe) having a plunger, wherein the syringe is connected to the transfer carousel such that the movement of the transfer carousel results in movement of the syringe and wherein the syringe is capable of translational movement relative to the transfer carousel; a drive motor connected to the syringe that is capable of controlling the position of the plunger; and a peripheral module comprising at least one vessel that is capable of containing a fluid, wherein the vessel has an opening that can be mated with the syringe to allow fluid transfer between the vessel and the syringe. Methods for transferring a fluid are also disclosed.

公开(公告)号：US09889446B2

公开(公告)日：2018-02-13

申请号：US15586896

申请日：2017-05-04

申请人： The University of Chicago

发明人： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC分类号： B01L3/00 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  G01N35/08 ,  C12P19/34 ,  C12Q1/44 ,  B01F3/08 ,  B01L7/00 ,  B82Y30/00 ,  G01N15/14

CPC分类号： B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

摘要： The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

公开(公告)号：US20170131279A1

公开(公告)日：2017-05-11

申请号：US15415290

申请日：2017-01-25

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Michael L. Samuels

IPC分类号： G01N33/573 ,  G01N33/542 ,  C12Q1/68 ,  G01N33/58

CPC分类号： G01N33/573 ,  B01F3/0807 ,  B01F5/0646 ,  B01F5/0653 ,  B01F13/0062 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  G01N2500/00

摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US09463431B2

公开(公告)日：2016-10-11

申请号：US14928607

申请日：2015-10-30

申请人： President and Fellows of Harvard College

发明人： J. Christopher Love ,  Hidde L. Ploegh ,  Jehnna Ronan

IPC分类号： G01N33/53 ,  B01J19/00 ,  G01N33/543 ,  B01L3/00 ,  G01N21/64 ,  B82Y30/00 ,  G01N33/50 ,  G01N33/68 ,  G01N33/577 ,  G01N33/531

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01J2219/0074 ,  B01L3/50853 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B82Y30/00 ,  G01N21/6452 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/531 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/577 ,  G01N33/6854 ,  Y10S436/809

摘要： Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.

摘要翻译： 提供了筛选测定和进行这种测定的方法。 在某些实施例中，可以设计测定和方法以确定两种或更多种类是否可以彼此结合。 在一些实例中，测定和方法可用于确定已知抗原是否与未知单克隆抗体结合。

公开(公告)号：US09410953B2

公开(公告)日：2016-08-09

申请号：US13122228

申请日：2009-09-30

申请人： Charles R. Mace ,  Amrita R. Yadav ,  Benjamin L. Miller

发明人： Charles R. Mace ,  Amrita R. Yadav ,  Benjamin L. Miller

IPC分类号： C40B50/14 ,  C40B30/04 ,  C40B40/06 ,  G01N33/543 ,  B01J19/00 ,  C12Q1/68

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/00533 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01J2219/0074 ,  C12Q1/6837 ,  G01N33/54353

摘要： The present invention relates to a formulations and methods for coupling a reactant (or probe precursor) to a functionalized surface for purposes of forming an arrayed sensor. This method includes the steps of: providing a surface having a reactive functional group; and introducing onto the surface, at a plurality of discrete locations, two or more compositions of the invention, which include a different reactant (probe precursor) and a non-nucleophilic additive, wherein such introduction is carried out under conditions effective to allow for covalent binding of the reactant to the surface via the reactive functional group. This results in a probe-functionalized array that substantially overcomes the problem of surface morphological anomalies on the array surface. Use of the resulting arrays in various detection systems is also encompassed.

摘要翻译： 本发明涉及用于将反应物（或探针前体）偶联到官能化表面以用于形成阵列传感器的配方和方法。 该方法包括以下步骤：提供具有反应性官能团的表面; 并在多个离散位置上引入本发明的两种或更多种组合物，其包括不同的反应物（探针前体）和非亲核性添加剂，其中这种引入在有效允许共价的条件下进行 通过反应性官能团将反应物与表面结合。 这导致探针官能化的阵列，其基本上克服了阵列表面上的表面形态异常的问题。 还包括在各种检测系统中使用所得到的阵列。

公开(公告)号：US09310305B2

公开(公告)日：2016-04-12

申请号：US13980721

申请日：2012-02-06

申请人： Nicolas Demierre

发明人： Nicolas Demierre

IPC分类号： G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC分类号： G01N21/6486 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00558 ,  B01J2219/0056 ,  B01J2219/00576 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/021 ,  B01L2300/0832 ,  G01N33/54313

摘要： The present invention relates to an encoded microcarrier comprising a readable code attached to it for identification, said encoded microcarrier comprising a body having at least a detection surface to detect a chemical and/or biological reaction, the microcarrier further comprising at least a spacing element projecting from the body and shaped to ensure that, when the encoded microcarrier is laid on a flat plane with the detection surface facing said flat plane, a gap exists between said flat plane and the detection surface. The invention also relates to an assay device designed to use a plurality of said encoded microcarriers to perform assays. The invention relates finally to a method for monitoring a chemical or biological reaction.

摘要翻译： 本发明涉及一种编码的微载体，其包括附着于其的用于识别的可读代码，所述编码微载体包括至少具有检测表面以检测化学和/或生物反应的主体，所述微载体还包括至少一个间隔元件突出 并且成形以确保当将编码的微载体放置在具有面向所述平面的检测表面的平坦平面上时，在所述平面和检测表面之间存在间隙。 本发明还涉及设计用于使用多个所述编码的微载体进行测定的测定装置。 本发明最终涉及用于监测化学或生物反应的方法。