公开(公告)号：US20240280573A1

公开(公告)日：2024-08-22

申请号：US18469907

申请日：2023-09-19

Applicant: California Institute of Technology

Inventor： Rong Fan ,  Habib Ahmad ,  James R. Heath

IPC: G01N33/543 ,  B01J19/00 ,  B01L3/00 ,  G01N33/58

CPC classification number: G01N33/54393 ,  B01J19/0046 ,  B01L3/502746 ,  B01L3/502753 ,  G01N33/54366 ,  G01N33/582 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N2458/10

Abstract: Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

公开(公告)号：US11754499B2

公开(公告)日：2023-09-12

申请号：US16780508

申请日：2020-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: B01L3/00 ,  G01N21/64 ,  B01J19/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  B01L7/00

CPC classification number: G01N21/6445 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01J2219/0059 ,  B01J2219/0065 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N2500/00 ,  C12Q1/6827 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/131 ,  C12Q2563/159 ,  C12Q1/6804 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/159 ,  C12Q1/6851 ,  C12Q2563/159 ,  C12Q2561/113 ,  C12Q2565/1015

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.

公开(公告)号：US11701654B2

公开(公告)日：2023-07-18

申请号：US17992784

申请日：2022-11-22

Applicant: Cellares Corporation

Inventor： Vladimir Azersky ,  Fabian Gerlinghaus ,  Daniele Malleo ,  Brian Alexander Pesch ,  Bharat S. Thakkar ,  Wilson Wai Toy

IPC: B01L3/00 ,  A61L2/04 ,  A61L2/20 ,  A61L2/08 ,  B01J19/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/36 ,  C12N5/00

CPC classification number: B01L3/502715 ,  A61L2/04 ,  A61L2/20 ,  A61L2/206 ,  A61L2/208 ,  B01J19/004 ,  B01L13/00 ,  C12M23/44 ,  C12M23/50 ,  C12M41/48 ,  C12N5/00 ,  C12N5/0081 ,  A61L2/08 ,  A61L2202/11 ,  A61L2202/122 ,  A61L2202/123 ,  A61L2202/14 ,  B01J2219/0002 ,  B01J2219/0074 ,  B01L2200/027 ,  B01L2300/049

Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

公开(公告)号：US20230149922A1

公开(公告)日：2023-05-18

申请号：US17992784

申请日：2022-11-22

Applicant: Cellares Corporation

Inventor： Bharat S. THAKKAR ,  Fabian GERLINGHAUS ,  Brian Alexander PESCH

IPC: B01L3/00 ,  B01J19/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/36 ,  A61L2/04 ,  A61L2/20 ,  C12N5/00

CPC classification number: B01L3/502715 ,  B01L13/00 ,  B01J19/004 ,  C12M23/44 ,  C12M23/50 ,  C12M41/48 ,  A61L2/04 ,  A61L2/208 ,  A61L2/20 ,  A61L2/206 ,  C12N5/00 ,  C12N5/0081 ,  B01L2200/027 ,  B01L2300/049 ,  B01J2219/0002 ,  B01J2219/0074 ,  A61L2202/14 ,  A61L2202/122 ,  A61L2202/11 ,  A61L2/08

Abstract: Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

公开(公告)号：US20180326383A1

公开(公告)日：2018-11-15

申请号：US15969972

申请日：2018-05-03

Applicant: MAK ENGINEERING CORPORATION ,  RIGAKU KOBO, LLC

Inventor： Isao Kotani ,  Nobuyuki Nakayama

IPC: B01J8/10 ,  B01J19/00 ,  B01F13/08

CPC classification number: B01J8/10 ,  B01F13/0818 ,  B01F13/1022 ,  B01J19/0066 ,  B01J2219/00004 ,  B01J2219/00479 ,  B01J2219/0074

Abstract: A compact desktop continuous stirred tank reactor easily used on a magnetic stirrer is provided. A desktop continuous stirred tank reactor used on a magnetic stirrer includes a plurality of containers, each of the plurality of containers having a bottom and a shape capable of containing a stir bar, the plurality of containers being configured as a single unit member. The plurality of containers is arranged on the circumference of a circle of rotation of a pair of magnets of the magnetic stirrer or inside the circumference, and adjacent containers communicate through communication holes.

公开(公告)号：US20180245133A1

公开(公告)日：2018-08-30

申请号：US15965155

申请日：2018-04-27

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Matthew T. O'Keefe ,  Pamela K. Foreman

IPC: C12Q1/686 ,  C12Q1/6883

CPC classification number: C12Q1/686 ,  B01F13/0059 ,  B01F13/0071 ,  B01F13/0084 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0431 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/50857 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/10 ,  B01L2300/165 ,  B01L2400/0688 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  C40B40/06 ,  C40B60/14 ,  H01J49/0418 ,  Y02P20/582

Abstract: Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.

公开(公告)号：US20180172595A1

公开(公告)日：2018-06-21

申请号：US15898283

申请日：2018-02-16

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Jonathan K. Leland ,  Mark A. Billadeau ,  Joseph M. Leginus ,  Bandele Jeffrey-Coker ,  Jeff D. Debad ,  Koustubh A. Phalnikar ,  Sriram Jambunathan

IPC: G01N21/76 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/00 ,  G01N27/30 ,  G01N33/543

CPC classification number: G01N21/76 ,  B01J19/0046 ,  B01J2219/00653 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/5029 ,  B01L9/527 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/049 ,  B01L2400/0688 ,  B33Y80/00 ,  C12Q1/001 ,  C40B40/10 ,  G01N27/30 ,  G01N33/5438 ,  G01N2458/30 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

Abstract: Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.

公开(公告)号：US20180120310A1

公开(公告)日：2018-05-03

申请号：US15852939

申请日：2017-12-22

Applicant: Aushon Biosystems, Inc.

Inventor： Peter HONKANEN ,  Christine A. BURNS

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54393 ,  B01J2219/00315 ,  B01J2219/00385 ,  B01J2219/00576 ,  B01J2219/00693 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5085 ,  B01L2200/148 ,  B01L2300/0636 ,  B01L2300/0829 ,  G01N33/54366

Abstract: An apparatus and a method are disclosed for printing in-well calibration features onto assay substrates. An apparatus includes a testing substrate; a plurality of capture compound features in a well of the testing substrate; a calibration feature on one of the capture compound features in the well of the testing substrate, where the calibration feature has a known concentration of a compound that is capable of binding to the capture compound; and at least one additional capture compound feature in the same well of the testing substrate, where the at least one additional capture compound feature does not have a calibration feature printed onto the at least one additional capture compound feature. Methods for using the same are disclosed.

公开(公告)号：US20180051243A1

公开(公告)日：2018-02-22

申请号：US15557706

申请日：2016-03-11

Applicant: The Trustees of the University of Pennsylvania

Inventor： Michael HOGAN ,  Orkan M. TELHAN ,  Karen HOGAN

IPC: C12M1/26 ,  C12M1/36 ,  C12M1/42 ,  C12M1/34 ,  C12M1/00 ,  C12M3/00

CPC classification number: C12M33/04 ,  B01J19/0046 ,  B01J2219/0029 ,  B01J2219/00326 ,  B01J2219/00353 ,  B01J2219/00376 ,  B01J2219/0054 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/0074 ,  C12M23/44 ,  C12M23/50 ,  C12M31/04 ,  C12M35/02 ,  C12M41/34 ,  C12M41/46 ,  C12M41/48 ,  C12M47/06 ,  C12M47/12

Abstract: A fluid transfer system includes a transfer carousel capable of rotational and/or translational movement; at least one holding vessel (e.g. syringe) having a plunger, wherein the syringe is connected to the transfer carousel such that the movement of the transfer carousel results in movement of the syringe and wherein the syringe is capable of translational movement relative to the transfer carousel; a drive motor connected to the syringe that is capable of controlling the position of the plunger; and a peripheral module comprising at least one vessel that is capable of containing a fluid, wherein the vessel has an opening that can be mated with the syringe to allow fluid transfer between the vessel and the syringe. Methods for transferring a fluid are also disclosed.

公开(公告)号：US09889446B2

公开(公告)日：2018-02-13

申请号：US15586896

申请日：2017-05-04

Applicant: The University of Chicago

Inventor： Rustem F. Ismagilov ,  Joshua David Tice ,  Helen Song Baca ,  Lewis Spencer Roach

IPC: B01L3/00 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  G01N35/08 ,  C12P19/34 ,  C12Q1/44 ,  B01F3/08 ,  B01L7/00 ,  B82Y30/00 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01F3/0861 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0071 ,  B01F2215/0037 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00736 ,  B01J2219/0074 ,  B01J2219/00756 ,  B01J2219/00783 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00975 ,  B01J2219/00977 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0424 ,  B01L2400/0448 ,  B01L2400/0454 ,  B01L2400/0487 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/44 ,  G01N15/1404 ,  G01N15/1484 ,  G01N35/08 ,  G01N35/085 ,  G01N2015/1409 ,  Y02A90/26 ,  Y10T436/118339 ,  Y10T436/12 ,  Y10T436/2575

Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.