公开(公告)号：US4801691A

公开(公告)日：1989-01-31

申请号：US50146

申请日：1987-05-15

申请人： Henry E. Auer

发明人： Henry E. Auer

IPC分类号： C07K1/30 ,  C07K3/24 ,  C07K3/28

CPC分类号： C07K1/30

摘要： Guanidine hydrochloride (GCl) is used to remove excess sodium dodecyl sulfate (SDS) from SDS-solubilized protein solutions, and particularly from SDS-solubilized inclusion body solutions, GCl is added to the solution containing SDS to induce the formation of a GCl-SDS complex (GDS) which, when allowed to precipitate, can easily be removed by centrifugation, filtration, or other suitable means.

公开(公告)号：US5849694A

公开(公告)日：1998-12-15

申请号：US553511

申请日：1990-07-16

申请人： Richard M. Synenki ,  Peter M. Rubinelli ,  Henry E. Auer ,  James E. Seely

发明人： Richard M. Synenki ,  Peter M. Rubinelli ,  Henry E. Auer ,  James E. Seely

IPC分类号： A61K38/27 ,  A61K38/00 ,  C07K14/61 ,  C12N15/09 ,  C12N15/18 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/61 ,  A61K38/00 ,  Y10S930/12

摘要： The present invention provides a stable and bioactive recombinant small-loop modified somatotropin which has its small-loop cysteines changed to other amino acids or deleted altogether and a method for producing such a small-loop modified somatotropin. Also provided by the present invention are DNA sequences coding for such small-loop modified somatotropins, plasmids containing such sequences, and organisms containing such plasmids. Furthermore, pharmaceutical compositions including small-loop modified somatotropins and methods for using such pharmaceutical compositions are disclosed.

摘要翻译： 本发明提供一种稳定和生物活性的重组小环修饰生长激素，其小环半胱氨酸改变为其它氨基酸或全部缺失，以及一种生产这种小环修饰生长激素的方法。 本发明还提供了编码这种小环修饰生长激素的DNA序列，含有此类序列的质粒和含有这种质粒的生物体。 此外，公开了包含小环修饰生长激素的药物组合物和使用这种药物组合物的方法。

公开(公告)号：US06514533B1

公开(公告)日：2003-02-04

申请号：US08934830

申请日：1997-09-22

申请人： Paul A. Burke ,  Stephen E. Zale ,  Mark A. Tracy ,  OluFunmi Lily Johnson ,  Howard Bernstein ,  M. Amin Khan ,  Henry E. Auer

发明人： Paul A. Burke ,  Stephen E. Zale ,  Mark A. Tracy ,  OluFunmi Lily Johnson ,  Howard Bernstein ,  M. Amin Khan ,  Henry E. Auer

IPC分类号： A61K910

CPC分类号： A61K9/5153 ,  A61K9/0019 ,  A61K9/1611 ,  A61K9/1647 ,  A61K9/1658 ,  A61K9/1694 ,  A61K9/5031 ,  A61K9/5052 ,  A61K38/1816 ,  A61K38/212 ,  A61K38/27 ,  A61K47/02 ,  A61K47/26 ,  C07K14/56 ,  Y10S514/97

摘要： A device for the sustained release in vivo of a water soluble, biologically active agent wherein the agent is susceptible to aggregation comprising a drug delivery device and aggregation-stabilized, biologically active agent wherein the aggregation-stabilized agent is disposed within the drug delivery device.

摘要翻译： 用于在体内持续释放水溶性生物活性剂的装置，其中所述试剂易于凝集，其包含药物递送装置和聚集稳定的生物活性剂，其中所述聚集稳定的试剂置于所述药物递送装置内。

公开(公告)号：US06569458B1

公开(公告)日：2003-05-27

申请号：US08006682

申请日：1993-01-21

申请人： Wayne R. Gombotz ,  Michael S. Healy ,  Larry R. Brown ,  Henry E. Auer

发明人： Wayne R. Gombotz ,  Michael S. Healy ,  Larry R. Brown ,  Henry E. Auer

IPC分类号： A61K916

CPC分类号： A61K9/1694 ,  A61K9/1688

摘要： Small diameter particles of biologically active molecules are produced by atomizing solutions of the molecules through a nozzle into very cold liquified gases, which immediately freeze the atomized droplets. The resulting frozen particles having diameters of approximately 10 to 90 micrometers are lyophilized to produce porous particles, suspended in a non-solvent for the molecules, and exposed to ultrasonic energy or homogenization to produce particles having diameters of 0.1 to 10 micrometers with greater than 70 to 95% of the initial biological activity of the molecules in solution.

摘要翻译： 生物活性分子的小直径颗粒通过将分子的溶液通过喷嘴雾化成非常冷的液化气体而产生，其立即冷冻雾化的液滴。 将所得直径约为10至90微米的冷冻颗粒冻干以产生悬浮于分子的非溶剂中的多孔颗粒，并暴露于超声波能量或均化，以产生直径为0.1至10微米的颗粒，大于70 至溶液中分子的初始生物活性的95％。