公开(公告)号：US07998694B2

公开(公告)日：2011-08-16

申请号：US12065210

申请日：2006-08-29

申请人： Eric Trinquet ,  Hervé Ansanay ,  Gérard Mathis

发明人： Eric Trinquet ,  Hervé Ansanay ,  Gérard Mathis

IPC分类号： G01N33/567

CPC分类号： G01N33/542 ,  G01N33/6872 ,  Y10S435/968

摘要： The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps: incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane; exciting the measurement medium at the excitation wavelength of the energy-donating member; and measuring the FRET signal or the variations in said signal emitted in said culture medium.

摘要翻译： 本发明涉及使用FRET测定揭示生物过程的方法，其包括以下步骤：将含有脂质膜的生物体X与一对FRET伴侣的第一成员结合， 与该对FRET伴侣的第二成员结合的第二生物实体Y，该对FRET伴侣中的能量供给成员具有长寿命，并且所述一对FRET伴侣的成员位于脂质膜的任一侧 ; 在能量供给构件的激发波长下激发测量介质; 以及测量FRET信号或在所述培养基中发射的所述信号的变化。

公开(公告)号：US08178704B2

公开(公告)日：2012-05-15

申请号：US11792135

申请日：2005-12-02

申请人： Hervé Bazin ,  Hervé Ansanay ,  Eric Trinquet ,  Gérard Mathis

发明人： Hervé Bazin ,  Hervé Ansanay ,  Eric Trinquet ,  Gérard Mathis

IPC分类号： C07F9/02 ,  G01N33/53

CPC分类号： C07F9/6561 ,  C07F9/117 ,  C07F9/572 ,  C07F9/65517 ,  C07F9/65583

摘要： The present invention relates to inositol phosphate derivatives, in which the inositol phosphate is substituted with one or two reactive groups G or one or two conjugated substances or molecules M, said reactive group(s) G or said substance(s) or molecule(s) M being linked to IP1 via a linkage group L, M being chosen from the following group: a tracer, an immunogen, a member of a binding partner pair, a solid support.Application: tools allowing the study of the inositol phosphate cycle and therefore, indirectly, the study of seven transmembrane domain receptors coupled to phospholipase C, receptors having a tyrosine kinase activity, and in general enzymes involved in the variations of the intracellular concentration of IP1.

摘要翻译： 本发明涉及肌醇磷酸酯衍生物，其中磷酸肌醇被一个或两个反应性基团G或一个或两个共轭物质或分子M取代，所述反应性基团G或所述物质或分子 ）M通过连接基团L连接到IP1，M选自以下组：示踪剂，免疫原，结合配偶体对的成员，固体支持物。 应用：允许研究肌醇磷酸循环的工具，因此间接研究偶联于磷脂酶C的七个跨膜结构域受体，具有酪氨酸激酶活性的受体，以及涉及IP1细胞内浓度变化的一般酶。