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公开(公告)号：US08546141B2
公开(公告)日：2013-10-01
申请号：US12935380
申请日：2009-04-01
申请人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
发明人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
CPC分类号： C12N5/0644 , A61K35/12 , C12N2501/125 , C12N2501/145 , C12N2501/165 , C12N2501/91 , C12N2502/1394 , C12N2506/45
摘要： A method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells is achieved in an in vitro culture system. A sac-like structure encloses hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, a method for producing various types of blood cells, comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, a method for producing various types of blood cells, particularly megakaryocytes and platelets, is achieved without involving the sac-like structure.
摘要翻译： 在体外培养系统中实现了从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 囊状结构包围造血祖细胞，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，生产各种血细胞的方法包括在适于诱导血细胞分化的条件下培养包封在囊样结构中的造血祖细胞。 此外，不涉及囊状结构，可以实现生产各种血细胞，特别是巨核细胞和血小板的方法。
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2.发明授权Sac-like structure enclosing hematopoietic progenitor cells produced from ES cells and method for preparing blood cells 有权封闭由ES细胞产生的造血祖细胞的囊状结构和用于制备血细胞的方法公开(公告)号：US08058064B2
公开(公告)日：2011-11-15
申请号：US12444237
申请日：2004-10-04
IPC分类号： C12N5/00
CPC分类号： C12N5/0644 , C12N2501/105 , C12N2501/155 , C12N2501/165 , C12N2502/13 , C12N2506/02
摘要： An object of the present invention is to provide a sac-like structure enclosing hematopoietic progenitor cells and a method for preparing the sac-like structure as well as a method for efficiently preparing blood cells such as mature megakaryocytes and platelets from the sac-like structure. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, the sac-like structure being obtained by plating ES cells onto feeder cells and culturing the ES cells under suitable conditions for inducing hematopoietic progenitor cell differentiation. Moreover, the present invention provides a method for producing various blood cells, the method comprising further culturing hematopoietic progenitor cells enclosed in the sac-like structure under suitable conditions for inducing blood cell differentiation.
摘要翻译： 本发明的目的是提供一种封闭造血祖细胞的囊状结构和制备囊状结构的方法以及从囊状结构有效地制备成熟巨核细胞和血小板的血细胞的方法 。 本发明提供封闭造血祖细胞的囊状结构，该囊状结构通过将ES细胞铺在饲养细胞上并在合适的条件下培养ES细胞以诱导造血祖细胞分化而获得。 此外，本发明提供了生产各种血细胞的方法，所述方法还包括在用于诱导血细胞分化的合适条件下包封在囊状结构中的造血祖细胞。
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公开(公告)号：US20110053267A1
公开(公告)日：2011-03-03
申请号：US12935380
申请日：2009-04-01
申请人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
发明人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
IPC分类号： C12N5/078 , C12N5/0789
CPC分类号： C12N5/0644 , A61K35/12 , C12N2501/125 , C12N2501/145 , C12N2501/165 , C12N2501/91 , C12N2502/1394 , C12N2506/45
摘要： It is an object of the present invention to provide a method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells in an in vitro culture system.The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, the present invention also provides a method for producing various types of blood cells, which comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, the present invention also provides a method for producing various types of blood cells, particularly megakaryocytes and platelets, without involving the sac-like structure.
摘要翻译： 本发明的目的是在体外培养系统中提供从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 本发明提供封闭造血祖细胞的囊状结构，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，本发明还提供了生产各种血细胞的方法，其包括在适于诱导血细胞分化的条件下培养包封在囊状结构中的造血祖细胞。 此外，本发明还提供了不涉及囊状结构来生产各种类型的血细胞，特别是巨核细胞和血小板的方法。
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4.发明申请Structure enclosing hematopoietic progenitor cells from ES cells and method for preparing blood cells using the same 有权结构围绕来自ES细胞的造血祖细胞和使用其制备血细胞的方法公开(公告)号：US20100197016A1
公开(公告)日：2010-08-05
申请号：US12444237
申请日：2007-10-04
IPC分类号： C12N5/078
CPC分类号： C12N5/0644 , C12N2501/105 , C12N2501/155 , C12N2501/165 , C12N2502/13 , C12N2506/02
摘要： An object of the present invention is to provide a sac-like structure enclosing hematopoietic progenitor cells and a method for preparing the sac-like structure as well as a method for efficiently preparing blood cells such as mature megakaryocytes and platelets from the sac-like structure. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, the sac-like structure being obtained by plating ES cells onto feeder cells and culturing the ES cells under suitable conditions for inducing hematopoietic progenitor cell differentiation. Moreover, the present invention provides a method for producing various blood cells, the method comprising further culturing hematopoietic progenitor cells enclosed in the sac-like structure under suitable conditions for inducing blood cell differentiation.
摘要翻译： 本发明的目的是提供一种封闭造血祖细胞的囊状结构和制备囊状结构的方法以及从囊状结构有效地制备成熟巨核细胞和血小板的血细胞的方法 。 本发明提供封闭造血祖细胞的囊状结构，该囊状结构通过将ES细胞铺在饲养细胞上并在合适的条件下培养ES细胞以诱导造血祖细胞分化而获得。 此外，本发明提供了生产各种血细胞的方法，所述方法还包括在用于诱导血细胞分化的合适条件下包封在囊状结构中的造血祖细胞。
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