公开(公告)号：US06858428B2

公开(公告)日：2005-02-22

申请号：US10214303

申请日：2002-08-08

申请人： Takao Saito ,  Sumio Maeda ,  Hidetoshi Inagaki

发明人： Takao Saito ,  Sumio Maeda ,  Hidetoshi Inagaki

IPC分类号： C12N9/74 ,  C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/077 ,  C12N5/09 ,  C12N5/10

CPC分类号： C12N5/0037 ,  C12N2501/70

摘要： The present invention provides low serum or serum free liquid culture media for human or animal cells, and the present invention relates to culture media containing antithrombin III and optionally heparin for the low-serum or serum-free culture of human or animal cells to suppress cell death. The antithrombin III is purified from human, bovine, or other animal serum, or antithrombin III derived from recombinant bacteria or cells containing the full length of the antithrombin III gene.

摘要翻译： 本发明提供用于人或动物细胞的低血清或无血清液体培养基，本发明涉及含有抗凝血酶III和任选的肝素用于人或动物细胞的低血清或无血清培养物以抑制细胞的培养基 死亡。 来自人，牛或其他动物血清的抗凝血酶III或衍生自重组细菌或含有全长抗凝血酶III基因的细胞的抗凝血酶III。

公开(公告)号：US06632668B2

公开(公告)日：2003-10-14

申请号：US09748186

申请日：2000-12-27

申请人： Sumio Maeda ,  Hidetoshi Inagaki ,  Takao Saito

发明人： Sumio Maeda ,  Hidetoshi Inagaki ,  Takao Saito

IPC分类号： C12N506

CPC分类号： C12N5/0671 ,  C12N2501/70 ,  C12N2501/999

摘要： A monolayer sheet structure is prepared containing a confluent monolayer of primary hepatocytes cultured on a substrate in sheet form. The monolayer sheet structure is formed by treating the hepatocytes in the monolayer formation stage with a protein-phosphorylation inhibitor having an indolo [2,3-a] carbazole structure, such as staurosporine, or other similar structure.

摘要翻译： 制备单层片材结构，其包含以片状形式在基材上培养的原代肝细胞汇合单层。 通过用具有吲哚并[2,3-a]咔唑结构的蛋白质 - 磷酸化抑制剂如星形孢菌素或其它类似结构，在单层形成阶段中处理肝细胞形成单层片结构。

公开(公告)号：US5200745A

公开(公告)日：1993-04-06

申请号：US591515

申请日：1990-10-02

申请人： Hideo Takai ,  Hidetoshi Inagaki ,  Toru Futami

发明人： Hideo Takai ,  Hidetoshi Inagaki ,  Toru Futami

IPC分类号： G05B19/042

CPC分类号： G05B19/0421 ,  G05B2219/2231 ,  G05B2219/25156 ,  G05B2219/25158

摘要： A system and method for communicating data between a master station and a plurality of control units applicable to an automotive vehicle are disclosed in which if an echoback communication in response to a control command is not received by the master station from the selected control unit within a predetermined period of time upon issuance of a continue command or a diagnostic execution command, the controlled state of the electronic control unit is released. Furthermore, to effectively diagnose the control operation of one of the control units interactively with the operation of other control units, an interrupt command may be issued while the selected control unit continues the predetermined control operation, and the remaining control units are then initialized. A different control unit may then be selected and set to a controlled state. While the second selected control unit is in the controlled state an interrupt command may be issued, thus maintaining the second selected control unit in a controlled state. A resume command is then issued to resume communication with the first selected control unit which has been maintained in a controlled state, so that the control operation of a control unit which is affected by the operation of any one of the other control units can appropriately be diagnosed.