公开(公告)号：US5340461A

公开(公告)日：1994-08-23

申请号：US12194

申请日：1993-02-02

申请人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

发明人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

IPC分类号： B01D57/02 ,  G01N27/447 ,  C25B9/00

CPC分类号： G01N27/44704 ,  B01D57/02 ,  G01N27/44747

摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.

摘要翻译： 最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。 凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。 板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的 功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。 电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有 非常难以切割，不会对凝胶造成伤害。