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1.发明申请Manufacturing Method of Black Vinegar and Black Vinegar Manufactured by the Method 审中-公开通过该方法制造的黑醋和黑醋的制造方法公开(公告)号：US20090047383A1
公开(公告)日：2009-02-19
申请号：US12085019
申请日：2006-06-27
IPC分类号： C12J1/04
摘要： An object of the present invention is to provide a manufacturing method of black vinegar which contains sugar derived from rice and barley abundantly, and is free from precipitation, and black vinegar manufactured by the method.The present invention provide a manufacturing method of black vinegar having the sugar content of 8-50 weight/volume % derived from rice and/or barley, wherein acetic acid fermentation is performed by mixing a saccharified solution and an alcoholic fermented broth of rice and/or barley, and the final concentration of oxalic acid in black vinegar is 36 ppm or less, and preferably 30 ppm or less.In addition, as a decreasing method of oxalic acid in black vinegar, storing with stirring a saccharified solution of rice and/or barley or a fermented broth after completion of acetic acid fermentation is effective. And the present invention provides black vinegar manufactured by these methods in which oxalate precipitation is hardly to occur, and which is suitable for drinking.
摘要翻译： 本发明的目的是提供一种黑醋的制造方法，该方法含有大米和大麦中衍生的糖并且没有沉淀，并且通过该方法制造的黑醋。 本发明提供从米和/或大麦得到的糖含量为8〜50重量/体积％的黑醋的制造方法，其中通过将糖化溶液与酒精发酵的水稻和/ 或大麦，黑醋中的草酸的最终浓度为36ppm以下，优选为30ppm以下。 此外，作为黑醋中草酸的降低方法，在乙酸发酵完成后，搅拌储存米和/或大麦或发酵液的糖化溶液是有效的。 本发明提供通过这些方法制造的黑醋，其中几乎不发生草酸盐沉淀，并且适合于饮用。
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2.发明授权
公开(公告)号：US5970163A
公开(公告)日：1999-10-19
申请号：US660687
申请日：1996-05-31
摘要： A method of measuring microbial activity of microbes in real time includes preparing a medium to enable microbial activity and placing the medium in operative contact with an appropriate sensor. The sensor is scanned to provide measurement electrical signals representative of position and amount of microbial activity, the sensor being responsive to a by-product of the microbial activity, such as a change in pH. The measurement signals can then be processed to provide a visual image display, and the display can visually distinguish between levels of pH activity and coordinate positions of the microbial activity.
摘要翻译： 实时测量微生物的微生物活性的方法包括制备培养基以实现微生物活性并使培养基与适当的传感器操作接触。 扫描传感器以提供表示微生物活性的位置和量的测量电信号，所述传感器响应于微生物活性的副产物，例如pH的变化。 然后可以处理测量信号以提供视觉图像显示，并且显示可以在视觉上区分pH活性的水平和微生物活动的坐标位置。
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公开(公告)号：US5085851A
公开(公告)日：1992-02-04
申请号：US552533
申请日：1990-07-16
CPC分类号： A61K8/66 , A61Q11/00 , Y10S514/90 , Y10S514/901 , Y10S514/902
摘要： A dental plaque-degrading composition containing endodextranase produced by Arthrobacter globiformis W31 exhibiting high degradation efficacy to insoluble glucan produced by Streptococcus mutans IFO 13955, the dental plaque-degrading composition additionally containing .alpha.-amylase of various origins. These compositions are useful for preventing the settlement of Streptococcus mutans in the oral cavity and the formation of new dental plaque and for preventing the dental caries, and provided in the form of dentifrices, denture detergents, troches, mouth washes, chewing gums or candies.
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4.发明授权Klebsella oxytoca ferm BP-I/3616 and immobilization thereof with a gelating agent in pores of a membrane 失效Klebsella oxytoca ferm BP-I / 3616，并用胶凝剂在膜的孔中固定
公开(公告)号：US5426042A
公开(公告)日：1995-06-20
申请号：US179593
申请日：1994-01-07
申请人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Keizo Hatagaki , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
发明人： Shigeru Maeda , Akira Ohki , Takeshi Sato , Naho Kato , Hirofumi Akano , Yoshiya Kawamura , Keizo Hatagaki , Yasushi Takahashi , Mikio Yamada , Hajime Okumura
CPC分类号： G01N33/1806 , C12Q1/001 , G01N33/1866 , G01N33/1893 , Y10S435/817 , Y10S435/852
摘要： A BOD analyzer is prepared having a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in the membrane. Specifically, the BOD analyzer has a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane, and a liquid passage which is connected with an entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from alginic acid or salts thereof, agar, gellan gum, xathane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, or pullulan. The BOD analyzer can be used for batch or continuous BOD analysis and enables carrying out BOD analysis in a short period of time.
摘要翻译： 制备具有含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，BOD分析装置具有配置有含有氧电极和微生物膜的微生物传感器的流通池，与流通池的入口连接并具有出口的液体通道。 通过使用至少一种选自藻酸或其盐，琼脂，结冷胶的凝胶剂，将属于克雷伯氏菌12092菌株的微生物直接固定在平均孔径为0.65-3μm的多孔亲水膜中来制备微生物膜 口香糖，xathane胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶或支链淀粉。 BOD分析仪可用于批量或连续BOD分析，可在短时间内进行BOD分析。
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公开(公告)号：US5726565A
公开(公告)日：1998-03-10
申请号：US511476
申请日：1995-08-04
申请人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
发明人： Shunichi Uchiyama , Takeshi Sato , Hirofumi Akano , Yoshiya Kawamura , Masahiro Fukaya , Hiroaki Ebisuya , Ko Furukawa , Kazuyo Kajino , Sumio Akita
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译： 本发明提供了用于定量测定样品物质的快速库仑分析，其中库仑分析具有高的再现性，而不需要外部来源的电压等的施加，以及用于所述的电池的电池和装置 库仑分析 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。
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6.发明授权Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter 失效用于电泳分离的电泳介质，用于保持相同介质的凝胶保持器，使用相同介质和凝胶保持器的板式电泳装置，以及电泳凝胶切割器
公开(公告)号：US5340461A
公开(公告)日：1994-08-23
申请号：US12194
申请日：1993-02-02
申请人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
发明人： Tomoaki Fukushige , Hiroko Toda , Hirofumi Akano , Emiko Ito , Tomohiko Fukuta , Masahiro Fujimori , Yoshiya Kawamura
IPC分类号： B01D57/02 , G01N27/447 , C25B9/00
CPC分类号： G01N27/44704 , B01D57/02 , G01N27/44747
摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.
摘要翻译： 最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。 凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。 板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的 功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。 电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有 非常难以切割，不会对凝胶造成伤害。
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7.发明授权
公开(公告)号：US5206170A
公开(公告)日：1993-04-27
申请号：US769534
申请日：1991-10-01
CPC分类号： C12Q1/005 , C12Q1/001 , G01N33/0042 , Y10S435/817
摘要： In a sulfur dioxide detector using SO.sub.2 -oxidant microbes, a bio-membrane is located at one end of an oxygen concentration sensor, the bio-membrane containing the SO.sub.2 -oxidant microbes between an immobilized membrane and a gas permeable membrane. The sensor has an electrode in contact with the immobilized membrane. A dish-shaped or frusto-conical cell has an open end which is in contact with the gas permeable membrane so that SO.sub.2 -laden solution supplied to the cell causes sulfurous acid to oxidize to sulfuric acid as the SO.sub.2 permeates through the bio-membrane so as to change an output from the sensor. An inlet and outlet hole are formed on a sidewall of the cell. The outlet hole is located to be always directly above the inlet hole so as to drain foam formed when the SO.sub.2 -laden solution is supplied to the cell through the inlet hole and drained from the outlet hole.
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公开(公告)号：US5909114A
公开(公告)日：1999-06-01
申请号：US957393
申请日：1997-10-23
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
摘要翻译： 本发明提供了一种用于定量测定样品物质的快速库仑分析，其重现性高，而没有外部施加电压等，以及用于所述库仑分析的电池和装置。 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。
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公开(公告)号：US5679572A
公开(公告)日：1997-10-21
申请号：US239698
申请日：1994-05-09
申请人： Yoshio Okamoto , Naoki Enomoto , Ryu Ohishi , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
发明人： Yoshio Okamoto , Naoki Enomoto , Ryu Ohishi , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
CPC分类号： B01J20/3219 , B01J20/286 , B01J20/289 , B01J20/29 , B01J20/3274 , C07B57/00 , C08B33/00 , C08B37/00 , Y10S435/803
摘要： Provided is a novel polysaccharide derivative having a main structure of the following formula (1) in which a polysaccharide or its derivative has been chemically bonded to the inner and outer surfaces of the pores of a porous carrier at the reducing terminal of the polysaccharide or polysaccharide derivative: ##STR1## Also provided are a method of producing the novel polysaccharide derivative in which a silane agent is chemically bonded to a porous carrier and thereafter a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is further chemically bonded to surface treated carrier at the reducing terminal of the polysaccharide or its derivative, and a method of producing the novel polysaccharide derivative in which a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is chemically bonded to a silane agent at the reducing terminal of the polysaccharide or its derivative and thereafter the polysaccharide derivative is further chemically bonded to a porous carrier. Further provided is a separating agent for chromatography containing the novel polysaccharide derivative. The novel polysaccharide derivative has excellent solvent resistance and is useful as a separating agent for chiral resolution of chiral compounds by chromatography. The methods of the present invention efficiently give the novel polysaccharide derivative.
摘要翻译： 本发明提供一种具有下述通式（1）的主要结构的新型多糖衍生物，其中多糖或其衍生物在多糖或多糖的还原端已经与多孔载体的孔的内表面和外表面化学键合 衍生物：
（1）+ TR 还提供了一种制备新型多糖衍生物的方法，其中硅烷试剂与多孔载体化学键合，然后是聚合度为11〜500的多糖或 其衍生物在多糖或其衍生物的还原末端进一步化学键合到表面处理的载体上，以及制备其聚合度为11〜500的多糖或其衍生物与其衍生物化学键合的新型多糖衍生物的方法 多糖或其衍生物的还原末端的硅烷试剂，之后是多糖衍生物 e进一步化学键合到多孔载体上。 还提供了含有新型多糖衍生物的色谱分离剂。 该新型多糖衍生物具有优异的耐溶剂性，可用作通过色谱手性拆分手性化合物的分离剂。 本发明的方法有效地提供了新型多糖衍生物。 -
公开(公告)号：US5496937A
公开(公告)日：1996-03-05
申请号：US239760
申请日：1994-05-09
申请人： Yoshio Okamoto , Naoki Enomoto , Sachiko Furukawa , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
发明人： Yoshio Okamoto , Naoki Enomoto , Sachiko Furukawa , Yasushi Ogasawara , Hirofumi Akano , Yoshiya Kawamura
CPC分类号： B01J20/3274 , B01J20/103 , B01J20/28004 , B01J20/28083 , B01J20/28085 , B01J20/29 , B01J20/3092 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3227 , B01J20/3246 , B01J20/3257 , B01J20/3259 , B01J20/3261 , B01J20/3263 , C07B57/00 , C08B37/00 , B01J2220/4825 , B01J2220/54 , B01J2220/58
摘要： A polysaccharide substance having the following formula is provided: ##STR1## Also provided is a method of producing the polysaccharide substance in which an oligosaccharide having a degree of polymerization from 3 to 10 is chemically bonded to a silane agent at the reducing terminal of the resultant oligosaccharide, the oligosaccharide compound is then polymerized to an average degree of polymerization from 11 to 500 in the presence of an enzyme. Another method for producing the polysaccharide substance is provided in which an aldehyde group at the reducing terminal of an oligosaccharide having a degree of polymerization from 3 to 10 is oxidized with an oxidizing agent, and the saccharide chain of the oxidized product is polymerized to a degree of polymerization from 11 to 500 in the presence of an enzyme, followed by the addition of an acid. The polysaccharide substance has excellent solvent resistance and is useful as a separating agent for chromatography, especially for the separation of chiral compounds.
摘要翻译： 提供具有下式的多糖物质：还提供了一种多糖物质的制造方法，其中聚合度为3〜10的寡糖与硅烷试剂化学键合 所得寡糖的还原末端，然后在酶的存在下将寡糖化合物聚合至平均聚合度为11至500。 提供另一种生产多糖物质的方法，其中将聚合度为3-10的低聚糖的还原末端的醛基用氧化剂氧化，并将氧化产物的糖链聚合到一定程度 在酶存在下从11-500聚合，然后加入酸。 多糖物质具有优异的耐溶剂性，可用作色谱分离剂，特别是手性化合物的分离。
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