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    Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter
    1.
    发明授权
    Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter 失效
    用于电泳分离的电泳介质，用于保持相同介质的凝胶保持器，使用相同介质和凝胶保持器的板式电泳装置，以及电泳凝胶切割器

    公开(公告)号：US5340461A

    公开(公告)日：1994-08-23

    申请号：US12194

    申请日：1993-02-02

    申请人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

    发明人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

    IPC分类号： B01D57/02 ,  G01N27/447 ,  C25B9/00

    CPC分类号： G01N27/44704 ,  B01D57/02 ,  G01N27/44747

    摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.

    摘要翻译： 最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。 凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。 板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的 功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。 电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有 非常难以切割，不会对凝胶造成伤害。

    Method for producing plastic base caps for split cavity type package semi-conductor units
    4.
    发明授权
    Method for producing plastic base caps for split cavity type package semi-conductor units 失效
    用于分裂腔型封装半导体单元的塑料底盖的制造方法

    公开(公告)号：US3981074A

    公开(公告)日：1976-09-21

    申请号：US499951

    申请日：1974-08-23

    申请人： Katsumi Yamamoto ,  Masahiro Fujimori ,  Masahiro Fujinawa ,  Sanenobu Sonoda

    发明人： Katsumi Yamamoto ,  Masahiro Fujimori ,  Masahiro Fujinawa ,  Sanenobu Sonoda

    IPC分类号： H01L21/50 ,  H01L23/057 ,  B01J17/00

    CPC分类号： H01L21/50 ,  H01L23/057 ,  H01L2224/45015 ,  H01L2224/45124 ,  H01L2224/48091 ,  H01L2224/48247 ,  H01L24/45 ,  H01L24/48 ,  H01L2924/01019 ,  H01L2924/14 ,  H01L2924/15153 ,  H01L2924/15165 ,  H01L2924/16195 ,  Y10T29/49121

    摘要： A method for producing a completed base cap for a plastic package sealing a semi-conductor therein includes the steps of forming the initial body of a plastic base cap including a centrally located hollow recess on one side thereof, placing lead frames on said base cap body in such a manner that the terminal end portions of said individual leads rest on the peripheral land of said base cap body surrounding said central recess, and forming outer side sections to the base cap including a step shoulder on the top side thereof by molding the same integrally with said lead frame and said initial body such that an intermediate portion of each of said leads adjacent their inner terminal end portions is embedded within said outer side sections of the base cap. A liquid honing step is provided on the base cap shoulder after the formation thereof, and to the inner terminal end portions of the leads.

    摘要翻译： 一种用于制造密封半导体的塑料封装的完整基座的方法包括以下步骤：在其一侧上形成包括位于中心的中空凹部的塑料基座的初始主体，将引线框架放置在所述基座主体 以使得所述单个引线的末端部分位于围绕所述中心凹部的所述基座主体的外围平台上，并且通过模制所述底盖而在其顶侧上形成包括台阶台肩的所述底盖的外侧部分 与所述引线框架和所述初始主体一体地使得每个所述引线的与其内部末端部分相邻的中间部分嵌入所述底盖的所述外侧部分内。 在其形成之后，在基座肩部上设置液体珩磨步骤，并且引线的内部末端部分。