公开(公告)号：US09366680B2

公开(公告)日：2016-06-14

申请号：US13060552

申请日：2008-08-28

申请人： Hirotaka Fujimoto ,  Toru Suzuki

发明人： Hirotaka Fujimoto ,  Toru Suzuki

IPC分类号： C07K16/26 ,  G01N33/68 ,  G01N33/74 ,  G01N33/543

CPC分类号： G01N33/6893 ,  C07K16/26 ,  C07K2317/34 ,  G01N33/543 ,  G01N33/74 ,  G01N2333/58 ,  G01N2800/32

摘要： The present invention provides a method and an index capable of less-invasively determining myocardial ischemia such as ischemic heart disease or restenosis after percutaneous coronary intervention. The present invention also provides an index that allows the cardiovascular disease other than heart failure to be determined even from a blood sample showing a BNP value from which the cardiovascular disease other than heart failure cannot be determined by a conventional method. A method for determining myocardial ischemia comprising subjecting a blood sample which is derived from a test subject and which contains a BNP molecular group containing at least two selected from the group consisting of BNP 1-32 molecule, BNP 3-32 molecule, BNP 4-32 molecule, BNP 5-32 molecule, and a molecule having a mass number larger than that of BNP 5-32 molecule by 16 Da to a detection process capable of distinguishing and quantifying the individual BNP molecules different in mass number to detect the BNP molecular group, wherein myocardial ischemia in the test subject is determined using, as an index, a ratio between a detected intensity of at least one molecule selected from the BNP molecular group and a detected intensity of at least one other molecule selected from the BNP molecular group.

摘要翻译： 本发明提供一种能够较少侵袭性地测定经皮冠状动脉介入治疗后缺血性心脏病或再狭窄等心肌缺血的方法和指标。 本发明还提供了即使从显示BNP值的血液样品中也可以确定心力衰竭以外的心血管疾病的指标，除了心力衰竭以外的心血管疾病不能通过常规方法来确定。 一种心肌缺血的测定方法，其特征在于，使来自受检对象的含有BNP分子组的BNP 1-32分子，BNP 3-32分子，BNP 4-32分子， 32分子，BNP 5-32分子，以及质量数大于BNP 5-32分子16Da的分子，能够区分和定量不同质量数的各BNP分子的检测方法，以检测BNP分子 组，其中使用选自BNP分子基团的至少一种分子的检测强度和至少一种选自BNP分子组的其它分子的检测强度之间的比率作为指标，测定受试者中的心肌缺血 。

公开(公告)号：US20110151583A1

公开(公告)日：2011-06-23

申请号：US13060552

申请日：2008-08-28

申请人： Hirotaka Fujimoto ,  Toru Suzuki

发明人： Hirotaka Fujimoto ,  Toru Suzuki

IPC分类号： G01N33/53

CPC分类号： G01N33/6893 ,  C07K16/26 ,  C07K2317/34 ,  G01N33/543 ,  G01N33/74 ,  G01N2333/58 ,  G01N2800/32

摘要： The present invention provides a method and an index capable of less-invasively determining myocardial ischemia such as ischemic heart disease or restenosis after percutaneous coronary intervention. The present invention also provides an index that allows the cardiovascular disease other than heart failure to be determined even from a blood sample showing a BNP value from which the cardiovascular disease other than heart failure cannot be determined by a conventional method. A method for determining myocardial ischemia comprising subjecting a blood sample which is derived from a test subject and which contains a BNP molecular group containing at least two selected from the group consisting of BNP 1-32 molecule, BNP 3-32 molecule, BNP 4-32 molecule, BNP 5-32 molecule, and a molecule having a mass number larger than that of BNP 5-32 molecule by 16 Da to a detection process capable of distinguishing and quantifying the individual BNP molecules different in mass number to detect the BNP molecular group, wherein myocardial ischemia in the test subject is determined using, as an index, a ratio between a detected intensity of at least one molecule selected from the BNP molecular group and a detected intensity of at least one other molecule selected from the BNP molecular group.

摘要翻译： 本发明提供一种能够较少侵袭性地测定经皮冠状动脉介入治疗后缺血性心脏病或再狭窄等心肌缺血的方法和指标。 本发明还提供了即使从显示BNP值的血液样品中也可以确定心力衰竭以外的心血管疾病的指标，除了心力衰竭以外的心血管疾病不能通过常规方法来确定。 一种心肌缺血的测定方法，其特征在于，使来自受检对象的含有BNP分子组的BNP 1-32分子，BNP 3-32分子，BNP 4-32分子， 32分子，BNP 5-32分子，以及质量数大于BNP 5-32分子16Da的分子，能够区分和定量不同质量数的各BNP分子的检测方法，以检测BNP分子 组，其中使用选自BNP分子基团的至少一种分子的检测强度和至少一种选自BNP分子组的其它分子的检测强度之间的比率作为指标，测定受试者中的心肌缺血 。

公开(公告)号：US08507286B1

公开(公告)日：2013-08-13

申请号：US12274928

申请日：2008-11-20

申请人： Hirotaka Fujimoto

发明人： Hirotaka Fujimoto

IPC分类号： G01N33/00

CPC分类号： G01N33/6848

摘要： Disclosed is a method of analyzing an oxidation state of a methionine residue in a protein sample, which comprises the steps of: inducing a reaction between a protein sample having a methionine residue with an oxidation state to be analyzed, and hydrogen peroxide H218O2 having oxygen atoms labeled by the isotope 18O, to obtain a modified protein sample in which the oxidation state of the methionine residue is stabilized; and subjecting the modified protein sample to a measurement to quantify an oxidation degree of the methionine residue. Preferably, the measurement is a mass spectrometric (MS) measurement using a mass spectrometer. The method can analyze an oxidation state of a methionine residue in a protein sample, in a simple manner, while accurately reflecting an in vivo oxidation state of the methionine residue.

摘要翻译： 公开了一种分析蛋白质样品中甲硫氨酸残基的氧化态的方法，其包括以下步骤：诱导具有甲硫氨酸残基的蛋白质样品与待分析的氧化态之间的反应，以及具有氧原子的过氧化氢H218O2 由同位素18O标记，得到其中甲硫氨酸残基的氧化态稳定的修饰蛋白质样品; 对经过修饰的蛋白质样品进行测定以定量甲硫氨酸残基的氧化度。 优选地，测量是使用质谱仪的质谱（MS）测量。 该方法可以简单地分析蛋白质样品中甲硫氨酸残基的氧化态，同时精确地反映蛋氨酸残基的体内氧化态。