公开(公告)号：US07588910B2

公开(公告)日：2009-09-15

申请号：US10557892

申请日：2004-05-21

申请人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma ,  Issei Yoshioka ,  Atsuhisa Nishimura ,  Homare Itou ,  Toshihiko Kumazawa ,  Takashi Kuroyanagi

发明人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma ,  Issei Yoshioka ,  Atsuhisa Nishimura ,  Homare Itou ,  Toshihiko Kumazawa ,  Takashi Kuroyanagi

IPC分类号： C12Q1/26 ,  C12Q1/37

CPC分类号： C12N9/0004 ,  C12N9/50 ,  C12Q1/37 ,  C12R1/01 ,  C12R1/07 ,  G01N33/66 ,  G01N33/723 ,  G01N2333/95

摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

摘要翻译： 提供了使用没有分离操作的酶来特异性测定糖化血红蛋白的糖基化β-链N-末端的方法及其测定试剂盒。 从糖基化的β-链N-末端切割糖化氨基酸和/或糖基化肽的蛋白酶，不从糖化血红蛋白或其片段的糖基化的α-链N末端基本上切割糖基化的氨基酸或糖化肽 被筛选 通过使用通过筛选方法获得的蛋白酶来提供糖基化血红蛋白的糖化β-链N-末端的方法和测定试剂盒。 根据本发明，糖化血红蛋白的糖化β-链N-末端可以在不进行分离操作的情况下进行确定。

公开(公告)号：US20080113381A1

公开(公告)日：2008-05-15

申请号：US10557892

申请日：2004-05-21

申请人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma ,  Issei Yoshioka ,  Atsuhisa Nishimura ,  Homare Itou ,  Toshihiko Kumazawa ,  Takashi Kuroyanagi

发明人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma ,  Issei Yoshioka ,  Atsuhisa Nishimura ,  Homare Itou ,  Toshihiko Kumazawa ,  Takashi Kuroyanagi

IPC分类号： G01N33/53 ,  C12N9/50 ,  C12N5/02 ,  C12N5/04 ,  C12Q1/37 ,  C12N9/06 ,  C07H21/04 ,  C12N15/00

CPC分类号： C12N9/0004 ,  C12N9/50 ,  C12Q1/37 ,  C12R1/01 ,  C12R1/07 ,  G01N33/66 ,  G01N33/723 ,  G01N2333/95

摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor.A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method.According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

摘要翻译： 提供了使用没有分离操作的酶来特异性测定糖化血红蛋白的糖基化β-链N-末端的方法及其测定试剂盒。 从糖基化的β-链N-末端切割糖化氨基酸和/或糖基化肽的蛋白酶，不从糖化血红蛋白或其片段的糖基化的α-链N末端基本上切割糖基化的氨基酸或糖化肽 被筛选 通过使用通过筛选方法获得的蛋白酶来提供糖基化血红蛋白的糖化β-链N-末端的方法和测定试剂盒。 根据本发明，糖化血红蛋白的糖化β-链N-末端可以在不进行分离操作的情况下进行确定。