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公开(公告)号：US20090170125A1
公开(公告)日：2009-07-02
申请号：US11919178
申请日：2006-04-27
申请人： Isabelle Heit , Monika Baudler , Sascha Dammeier , Michaela Schäfer , Hubert Paul , Gottfried Baier , Stephen Shaw
发明人： Isabelle Heit , Monika Baudler , Sascha Dammeier , Michaela Schäfer , Hubert Paul , Gottfried Baier , Stephen Shaw
IPC分类号： C12Q1/48 , G01N33/573 , C07K16/40
CPC分类号： G01N33/5041 , C07K16/40 , C07K16/44 , G01N2333/9121
摘要： The invention relates to a method for investigating the modulating effect of a test substance on a PKCθ-dependent signal transduction pathway or for finding a PKCθ modulator in a human or animal cell, including the steps (a) contacting the cell with the test substance or with the PKCθ modulator; (b) where appropriate inducing the kinase activity of PKCθ; (c) incubating the cell under conditions which bring about phosphorylation at least of a serine or threonine residue of PKCθ; (d) where appropriate lysing the cell; and (e) determining the phosphorylation content of the at least one serine or threonine residue of PKCθ.
摘要翻译： 本发明涉及一种用于研究测试物质对PKCθ依赖性信号转导途径或在人或动物细胞中发现PKCθ调节剂的调节作用的方法，包括步骤（a）使细胞与测试物质接触或 与PKCtheta调制器; （b）适当诱导PKCθ的激酶活性; （c）在至少引起PKCθ的丝氨酸或苏氨酸残基的磷酸化的条件下孵育细胞; （d）适当时裂解细胞; 和（e）确定PKCθ的至少一个丝氨酸或苏氨酸残基的磷酸化含量。
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公开(公告)号：US20120264624A1
公开(公告)日：2012-10-18
申请号：US13455568
申请日：2012-04-25
申请人： Bernd Drescher , Claudia Ehle , Leonhard Geissler , Hans-Juergen Mueller , Takenori Okusa , Hubert Paul
发明人： Bernd Drescher , Claudia Ehle , Leonhard Geissler , Hans-Juergen Mueller , Takenori Okusa , Hubert Paul
CPC分类号： B01F11/0002 , B01F11/0008 , B01F11/0037 , G01N1/38 , G01N35/025 , G01N2035/00158
摘要： A method for detecting analytes in a sample liquid is provided in which the sample liquid is subjected to a mixing treatment on a surface of a support having in particular immobilized reactants, wherein an in particular trough-like vessel with an essentially flat bottom as a support surface for the immobilized reactants is used as the vessel for the sample liquid. The sample liquid is moved relative to the immobilized reactants by an oscillating movement of the vessel during the mixing treatment in one direction, typically along a line or a curve. In addition, a device is provided for carrying out the method according to the present disclosure.
摘要翻译： 提供了一种用于检测样品液体中的分析物的方法，其中样品液体在特别是固定化的反应物的载体的表面上进行混合处理，其中特别是具有基本上平坦的底部作为载体的槽状容器 用于固定反应物的表面用作样品液体的容器。 样品液体通过容器在混合处理期间在一个方向（通常沿着一条线或曲线）的振荡运动相对于固定的反应物移动。 此外，提供了一种用于执行根据本公开的方法的设备。
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公开(公告)号：US3948091A
公开(公告)日：1976-04-06
申请号：US471898
申请日：1974-05-21
申请人： Hubert Paul Voll
发明人： Hubert Paul Voll
摘要： The apparatus includes a machine for subjecting a test-piece of superplastic material to a tensile test using a mobile member entraining one end of the test-piece and exerting traction on the test-piece. The mobile member is subjected to successive displacements whose speed and duration are controlled. The force applied to the test-piece and its length are measured as a function of time.
摘要翻译： 该设备包括一个机器，用于使用携带试样的一端的移动构件对试验片施加牵引力来对超塑性材料试件进行拉伸试验。 移动构件受到其速度和持续时间的控制的连续位移。 作为时间的函数测量施加到试件上的力及其长度。
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