公开(公告)号：US20230322872A1

公开(公告)日：2023-10-12

申请号：US17854870

申请日：2022-06-30

申请人： Hung-Chien CHIEN ,  Jason LU

发明人： Hung-Chien CHIEN ,  Jason LU

IPC分类号： C07K14/415 ,  C07K7/06 ,  C07K14/60 ,  C07K14/47 ,  C07K14/575 ,  C07K7/16

CPC分类号： C07K14/415 ,  C07K7/06 ,  C07K14/60 ,  C07K14/47 ,  C07K14/57545 ,  C07K7/16 ,  C07K14/57563 ,  C07K14/575 ,  C07K2319/00

摘要： A recombinant protein comprising multiple multi-peptide sets includes first through fifth sequencing primers and first through fourth multi-peptide regions. The first multi-peptide region is between the first and the second sequencing primers. The second multi-peptide region is between the second and the third sequencing primers. The third multi-peptide region is between the third and the fourth sequencing primers. The fourth multi-peptide region is between the fourth and the fifth sequencing primers. In each of the multi-peptide regions, multiple functional peptides can be inserted, and manufacturing thereof can be done through expression of the recombinant protein, thereby significantly enhancing the concentrations of the functional peptides. With the combination of peptide having different functions, the recombinant protein product can provide more complete and more comprehensive functionality. This application also discloses a pharmaceutical composition comprising the recombinant protein and a method for preparing the recombinant protein.

公开(公告)号：US20140186429A1

公开(公告)日：2014-07-03

申请号：US14023537

申请日：2013-09-11

申请人： Hung-Chien CHIEN

发明人： Hung-Chien CHIEN

IPC分类号： C07K2/00 ,  C12N9/02

CPC分类号： C07K2/00 ,  C12N9/0071 ,  C12Y114/16002

摘要： The present invention provides a method of biosynthesizing a recombinant protein containing the biologically active peptide, the pharmaceutical composition containing the recombinant protein as well as the preparation of the recombinant protein. Simply speaking the consecutively multiple copies of the bioactive peptide are replaced in the amino acid sequence of a recombinant protein (so called peptide-protein). Then, a high concentration and high yield of the peptide-protein containing the consecutively multiple copies of the bioactive peptide is produced by the biosafety and edible strain of yeast without any endotoxin contamination.

摘要翻译： 本发明提供了生物合成含有生物活性肽的重组蛋白质的方法，含有重组蛋白质的药物组合物以及重组蛋白质的制备。 简言之，在重组蛋白质（所谓的肽 - 蛋白质）的氨基酸序列中连续复制多个生物活性肽的拷贝。 然后，通过酵母的生物安全和可食用菌株产生含有连续多个生物活性肽拷贝的肽 - 蛋白质的高浓度和高收率，而没有任何内毒素污染。

公开(公告)号：US09233999B2

公开(公告)日：2016-01-12

申请号：US14023537

申请日：2013-09-11

申请人： Hung-Chien Chien

发明人： Hung-Chien Chien

IPC分类号： A61K38/00 ,  A61K38/03 ,  C07K2/00 ,  C12N9/02

CPC分类号： C07K2/00 ,  C12N9/0071 ,  C12Y114/16002

摘要： The present invention provides a method of biosynthesizing a recombinant protein containing the biologically active peptide, the pharmaceutical composition containing the recombinant protein as well as the preparation of the recombinant protein. Simply speaking the consecutively multiple copies of the bioactive peptide are replaced in the amino acid sequence of a recombinant protein (so called peptide-protein). Then, a high concentration and high yield of the peptide-protein containing the consecutively multiple copies of the bioactive peptide is produced by the biosafety and edible strain of yeast without any endotoxin contamination.

摘要翻译： 本发明提供了生物合成含有生物活性肽的重组蛋白质的方法，含有重组蛋白质的药物组合物以及重组蛋白质的制备。 简言之，在重组蛋白质（所谓的肽 - 蛋白质）的氨基酸序列中连续复制多个生物活性肽的拷贝。 然后，通过酵母的生物安全和可食用菌株产生含有连续多个生物活性肽拷贝的肽 - 蛋白质的高浓度和高收率，而没有任何内毒素污染。

公开(公告)号：US20230322886A1

公开(公告)日：2023-10-12

申请号：US17854918

申请日：2022-06-30

申请人： Hung-Chien CHIEN ,  Jason LU

发明人： Hung-Chien CHIEN ,  Jason LU

IPC分类号： C07K14/575 ,  C07K14/60 ,  C07K7/16 ,  C07K14/415 ,  C07K14/47 ,  C07K14/68 ,  C07K14/62

CPC分类号： C07K14/575 ,  C07K14/60 ,  C07K14/57545 ,  C07K7/16 ,  C07K14/57563 ,  A61K38/00 ,  C07K14/4732 ,  C07K14/68 ,  C07K14/62 ,  C07K14/47 ,  C07K2319/00 ,  C07K14/415

摘要： A recombinant protein comprising multiple multi-peptide sets includes first through fifth sequencing primers and first through fourth multi-peptide regions. The first multi-peptide region is between the first and the second sequencing primers. The second multi-peptide region is between the second and the third sequencing primers. The third multi-peptide region is between the third and the fourth sequencing primers. The fourth multi-peptide region is between the fourth and the fifth sequencing primers. In each of the multi-peptide regions, multiple functional peptides can be inserted, and manufacturing thereof can be done through expression of the recombinant protein, thereby significantly enhancing the concentrations of the functional peptides. With the combination of peptide having different functions, the recombinant protein product can provide more complete and more comprehensive functionality. This application also discloses a pharmaceutical composition comprising the recombinant protein and a method for preparing the recombinant protein.