Preparation of molecular imprinted polymers by cross-linking
    1.
    发明授权
    Preparation of molecular imprinted polymers by cross-linking 有权
    通过交联制备分子印迹聚合物

    公开(公告)号:US09212271B2

    公开(公告)日:2015-12-15

    申请号:US14379913

    申请日:2012-02-28

    摘要: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.

    摘要翻译: 提供了一种用于制备不溶性分子印迹聚合物(MIP)的改进方法,所述方法包括:a)提供可溶性或半溶性MIP,其1)基本上全部结合模板剂和2)具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法,b)交联在步骤a中提供的结合可溶性MIP的模板剂,以获得结合MIP的不溶性模板剂,以及c)任选分离,浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中,步骤a包括亲和纯化程序,其确保步骤a中提供的MIP确实是模板的所有结合物。

    PREPARATION OF MOLECULAR IMPRINTED POLYMERS BY CROSS-LINKING
    3.
    发明申请
    PREPARATION OF MOLECULAR IMPRINTED POLYMERS BY CROSS-LINKING 有权
    通过交联制备分子印迹聚合物

    公开(公告)号:US20150031779A1

    公开(公告)日:2015-01-29

    申请号:US14379913

    申请日:2012-02-28

    IPC分类号: C08J9/36

    摘要: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.

    摘要翻译: 提供了一种用于制备不溶性分子印迹聚合物(MIP)的改进方法,所述方法包括:a)提供可溶性或半溶性MIP,其1)基本上全部结合模板剂和2)具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法,b)交联在步骤a中提供的结合可溶性MIP的模板剂,以获得结合MIP的不溶性模板剂,以及c)任选分离,浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中,步骤a包括亲和纯化程序,其确保步骤a中提供的MIP确实是模板的所有结合物。