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公开(公告)号：US09149737B2
公开(公告)日：2015-10-06
申请号：US13496006
申请日：2010-09-16
CPC分类号： A61K31/74 , B01D15/1807 , B01D15/3804 , B01J20/26 , B01J20/268
摘要： Disclosed is a method for preparing a composition enriched for receptors (typically molecular impringet polymers, MIPs) that bind an agent, where said receptors each specifically bind at least two discrete sites on said agent, by subjecting a sample of receptors to a first step of affinity purification with the agent where one binding site on the agent is non-accessible for binding to the receptors and subsequently subjecting the purified receptors to at least one further step of affinity purification with the agent where a second binding site on the agent is non-accessible. Also disclosed is a method for treatment, amelioration or prophylaxis of a disease selected from the group consisting of phenylketonuria (PKU, Følling's disease), hyperphenylalaninemia (HPA), alcaptonuria (black urine disease), tyrosinemia, hypertyrosinemia, myasthenia gravis, histidinemia, urocanic aciduria, maple syrup urine disease (MSUD), isovaleric acidemia (isovaleryl-CoA dehydrogenase deficiency), homocystinuria, propionic acidemia, methylmalonic acidemia, and glutaric aciduria Type 1 (GA-I), galactosemia, comprising administering to the gastrointestinal tract of a patient in need thereof an effective amount of a composition of molecular imprinted polymers (MIPs), said composition being capable of binding a symptom provoking agent of said disease.
摘要翻译： 公开了一种制备富含结合试剂的受体（通常是分子侵入聚合物，MIP）的组合物的方法，其中所述受体各自特异性结合所述试剂上的至少两个离散位点，通过使受体样品经受第一步 与试剂亲和纯化，其中试剂上的一个结合位点不能与受体结合，然后使纯化的受体与试剂上的第二结合位点不相容的至少一个进一步的亲和纯化步骤进行， 无障碍。 还公开了治疗，改善或预防选自苯丙酮尿症（PKU，Følling氏病），高苯丙氨酸血症（HPA），尿酸血症（黑尿病），酪氨酸血症，高铁血症，重症肌无力，组氨酸血症，尿路感染 酸尿，枫糖尿病（MSUD），异戊酸 - 异丙酰脱氢酶缺乏症，同型胱尿蛋白尿，丙酸血症，甲基丙二酸血症和戊二酸1型糖尿病（GA-I），半乳糖血症，包括给予患者胃肠道 在有需要的情况下，有效量的分子印迹聚合物（MIP）的组合物，所述组合物能够结合所述疾病的症状发作剂。
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公开(公告)号：US09212271B2
公开(公告)日：2015-12-15
申请号：US14379913
申请日：2012-02-28
CPC分类号： C08J9/36 , B01D15/3804 , B01D15/3852 , B01J20/268 , B01J20/285 , B01J20/3007 , B01J20/3057 , B01J20/3085 , B01J2220/82 , B82Y30/00 , C08J2300/00
摘要： Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.
摘要翻译： 提供了一种用于制备不溶性分子印迹聚合物（MIP）的改进方法，所述方法包括：a）提供可溶性或半溶性MIP，其1）基本上全部结合模板剂和2）具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法，b）交联在步骤a中提供的结合可溶性MIP的模板剂，以获得结合MIP的不溶性模板剂，以及c）任选分离，浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中，步骤a包括亲和纯化程序，其确保步骤a中提供的MIP确实是模板的所有结合物。
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公开(公告)号：US20090104277A1
公开(公告)日：2009-04-23
申请号：US12279238
申请日：2007-02-21
CPC分类号： B01J20/26 , A61K31/74 , A61K47/58 , A61K47/593 , B01J20/268 , B01J20/3057
摘要： One aspect is a method for improved preparation of molecular imprinted polymer (MIP) particles, where initial compositions comprising insoluble MIP particles are enriched for those MIP particles that bind a particular target molecule, thus excluding non-binding and weakly binding particles from the final composition. Enrichment is typically accomplished via use of chromatographic methods capable of separating particulate material or by means of agglutination. Another aspect is preparation of improved insoluble MIPs by use of extended micronization of raw MIP particles with a view to expose a large number of binding sites per mass unit of MIP particles. In preferred embodiments the two aspects are combined. The resulting improved MIPs may be used for diagnostic, analytical and therapeutic purposes, notably as orally administered drugs which can bind substances such as cholesterol and bile acids and bile acid salts in the gastrointestinal tract.
摘要翻译： 一个方面是改进制备分子印迹聚合物（MIP）颗粒的方法，其中包含不溶性MIP颗粒的初始组合物富集用于结合特定靶分子的那些MIP颗粒，从而从最终组合物排除非结合和弱结合的颗粒 。 富集通常通过使用能够分离颗粒材料或通过凝集的色谱方法来实现。 另一方面是通过使用原始MIP颗粒的延长微粉化来制备改进的不溶性MIP，以期暴露每质量单位MIP颗粒的大量结合位点。 在优选实施例中，组合了两个方面。 所得到的改进的MIP可以用于诊断，分析和治疗目的，特别是作为口服给药的药物，其可以在胃肠道中结合胆固醇和胆汁酸和胆汁酸盐等物质。
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公开(公告)号：US20150031779A1
公开(公告)日：2015-01-29
申请号：US14379913
申请日：2012-02-28
IPC分类号： C08J9/36
CPC分类号： C08J9/36 , B01D15/3804 , B01D15/3852 , B01J20/268 , B01J20/285 , B01J20/3007 , B01J20/3057 , B01J20/3085 , B01J2220/82 , B82Y30/00 , C08J2300/00
摘要： Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.
摘要翻译： 提供了一种用于制备不溶性分子印迹聚合物（MIP）的改进方法，所述方法包括：a）提供可溶性或半溶性MIP，其1）基本上全部结合模板剂和2）具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法，b）交联在步骤a中提供的结合可溶性MIP的模板剂，以获得结合MIP的不溶性模板剂，以及c）任选分离，浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中，步骤a包括亲和纯化程序，其确保步骤a中提供的MIP确实是模板的所有结合物。
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公开(公告)号：US20120171154A1
公开(公告)日：2012-07-05
申请号：US13496006
申请日：2010-09-16
IPC分类号： A61K39/395 , A61P13/02 , A61K31/74
CPC分类号： A61K31/74 , B01D15/1807 , B01D15/3804 , B01J20/26 , B01J20/268
摘要： Disclosed is a method for preparing a composition enriched for receptors (typically molecular impringet polymers, MIPs) that bind an agent, where said receptors each specifically bind at least two discrete sites on said agent, by subjecting a sample of receptors to a first step of affinity purification with the agent where one binding site on the agent is non-accessible for binding to the receptors and subsequently subjecting the purified receptors to at least one further step of affinity purification with the agent where a second binding site on the agent is non-accessible. Also disclosed is a method for treatment, amelioration or prophylaxis of a disease selected from the group consisting of phenylketonuria (PKU, Følling's disease), hyperphenylalaninemia (HPA), alcaptonuria (black urine disease), tyrosinemia, hypertyrosinemia, myasthenia gravis, histidinemia, urocanic aciduria, maple syrup urine disease (MSUD), isovaleric acidemia (isovaleryl-CoA dehydrogenase deficiency), homocystinuria, propionic acidemia, methylmalonic acidemia, and glutaric aciduria Type 1 (GA-I), galactosemia, comprising administering to the gastrointestinal tract of a patient in need thereof an effective amount of a composition of molecular imprinted polymers (MIPs), said composition being capable of binding a symptom provoking agent of said disease.
摘要翻译： 公开了一种制备富含结合试剂的受体（通常是分子侵入聚合物，MIP）的组合物的方法，其中所述受体各自特异性结合所述试剂上的至少两个离散位点，通过使受体样品经受第一步 与试剂亲和纯化，其中试剂上的一个结合位点不能与受体结合，然后使纯化的受体与试剂上的第二结合位点不相容的至少一个进一步的亲和纯化步骤进行， 无障碍。 还公开了治疗，改善或预防选自苯丙酮尿症（PKU，Følling氏病），高苯丙氨酸血症（HPA），尿酸血症（黑尿病），酪氨酸血症，高铁血症，重症肌无力，组氨酸血症，尿路感染 酸尿，枫糖尿病（MSUD），异戊酸 - 异丙酰脱氢酶缺乏症，同型胱尿蛋白尿，丙酸血症，甲基丙二酸血症和戊二酸1型糖尿病（GA-I），半乳糖血症，包括给予患者胃肠道 在有需要的情况下，有效量的分子印迹聚合物（MIP）的组合物，所述组合物能够结合所述疾病的症状发作剂。
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