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公开(公告)号:US09212271B2
公开(公告)日:2015-12-15
申请号:US14379913
申请日:2012-02-28
CPC分类号: C08J9/36 , B01D15/3804 , B01D15/3852 , B01J20/268 , B01J20/285 , B01J20/3007 , B01J20/3057 , B01J20/3085 , B01J2220/82 , B82Y30/00 , C08J2300/00
摘要: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.
摘要翻译: 提供了一种用于制备不溶性分子印迹聚合物(MIP)的改进方法,所述方法包括:a)提供可溶性或半溶性MIP,其1)基本上全部结合模板剂和2)具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法,b)交联在步骤a中提供的结合可溶性MIP的模板剂,以获得结合MIP的不溶性模板剂,以及c)任选分离,浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中,步骤a包括亲和纯化程序,其确保步骤a中提供的MIP确实是模板的所有结合物。
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公开(公告)号:US20150031779A1
公开(公告)日:2015-01-29
申请号:US14379913
申请日:2012-02-28
IPC分类号: C08J9/36
CPC分类号: C08J9/36 , B01D15/3804 , B01D15/3852 , B01J20/268 , B01J20/285 , B01J20/3007 , B01J20/3057 , B01J20/3085 , B01J2220/82 , B82Y30/00 , C08J2300/00
摘要: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.
摘要翻译: 提供了一种用于制备不溶性分子印迹聚合物(MIP)的改进方法,所述方法包括:a)提供可溶性或半溶性MIP,其1)基本上全部结合模板剂和2)具有使其能够在色谱步骤中分离的尺寸 使用填充床层析法,b)交联在步骤a中提供的结合可溶性MIP的模板剂,以获得结合MIP的不溶性模板剂,以及c)任选分离,浓缩或纯化通过步骤b中的交联获得的MIP 。 在有趣的实施方案中,步骤a包括亲和纯化程序,其确保步骤a中提供的MIP确实是模板的所有结合物。
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3.
公开(公告)号:US06852818B1
公开(公告)日:2005-02-08
申请号:US10110235
申请日:2000-10-23
申请人: Sergiy Anatoliyovich Piletsky , Olena Volodimirivna Piletska , Ganna Valentinivna Elska , Hakan Syen Andersson , Ian Alan Nicholls , Anthony Peter Francis Turner
发明人: Sergiy Anatoliyovich Piletsky , Olena Volodimirivna Piletska , Ganna Valentinivna Elska , Hakan Syen Andersson , Ian Alan Nicholls , Anthony Peter Francis Turner
IPC分类号: A61K38/00 , A61K31/7088 , A61P7/00 , C07K1/04 , C07K14/00 , C08F2/00 , C08G69/10 , C08G85/00 , C08F26/00
CPC分类号: B01J20/26 , B01J20/268 , C07K14/001
摘要: A polymer (e.g. a peptide) is produced by polymerizing monomers in the presence of a template, particularly a biologically active molecule or sample. The polymer is extracted and a soluble (preferably water-soluble) fraction is derived which specifically binds the template. Thus it can have complementary activity (e.g. biological). Applications include therapy, detection systems and separation systems.
摘要翻译: 通过在模板,特别是生物活性分子或样品的存在下聚合单体来生产聚合物(例如肽)。 提取聚合物,得到特异性结合模板的可溶(优选水溶性)级分。 因此,它可以具有互补活性(例如生物学)。 应用包括治疗,检测系统和分离系统。
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