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    Methods for Flow Cytometry Analyses of Un-Lysed Cells from Biological Fluids
    1.
    发明申请
    Methods for Flow Cytometry Analyses of Un-Lysed Cells from Biological Fluids 审中-公开
    用于生物流体中未裂解细胞的流式细胞术分析方法

    公开(公告)号：US20090105963A1

    公开(公告)日：2009-04-23

    申请号：US12300342

    申请日：2007-05-11

    申请人： Jesper Laursen ,  Ian Storie

    发明人： Jesper Laursen ,  Ian Storie

    IPC分类号： G06F19/00

    CPC分类号： G01N33/56972 ,  G01N15/1475 ,  G01N33/5094

    摘要： A method for analyzing a pathological deviation of at least one white blood cell population from a normal level in an un-lysed blood sample, comprising the steps of counting, with a flow cytometer, a number, n1, of white blood cells expressing a first marker; a number, n2, of white blood cells expressing a second marker, and a number, n3, of white blood cells expressing a third marker but not the first marker; and comparing the sum (n1+n2+n3) with a reference value. The sum (n1+n2+n3) may represent the number of lymphocytes. The first, second and third markers may be chosen from the group consisting of CD56, CD3 and CD19.

    摘要翻译： 一种用于分析未裂解血样中至少一种白细胞群与正常水平的病理学偏差的方法，包括以下步骤：用流式细胞仪计数表达第一 标记 表达第二标记的白细胞数目n2，表达第三标记而不是第一标记的白细胞数n3; 并将和（n1 + n2 + n3）与参考值进行比较。 总和（n1 + n2 + n3）可以表示淋巴细胞数。 第一，第二和第三标记可以选自CD56，CD3和CD19。

    High-Speed Quantification of Antigen Specific T-Cells in Whole Blood by Flow Cytometry
    2.
    发明申请
    High-Speed Quantification of Antigen Specific T-Cells in Whole Blood by Flow Cytometry 审中-公开
    通过流式细胞术高效定量全血中抗原特异性T细胞

    公开(公告)号：US20090061478A1

    公开(公告)日：2009-03-05

    申请号：US12161579

    申请日：2007-01-30

    申请人： Lene Have Poulsen ,  Kivin Jacobsen ,  Ian Storie ,  Jesper Laursen

    发明人： Lene Have Poulsen ,  Kivin Jacobsen ,  Ian Storie ,  Jesper Laursen

    IPC分类号： C12Q1/06

    CPC分类号： G01N33/505 ,  G01N2015/0065 ,  G01N2015/1486

    摘要： The present invention discloses novel methods and compositions for identifying particular cell types in whole blood samples and defining either the concentration or ‘absolute count’ of these cells per unit volume of the sample. More specifically, the invention relates to methods for quantifying the antigen-specific T cells or defining the relative percentage of said cells in un-lysed whole blood. Further, the invention relates to kits for the preparation of whole blood samples for high-speed quantification of particular cell types, e.g. antigen specific T-cells, in said whole blood samples by flow cytometry.

    摘要翻译： 本发明公开了用于鉴定全血样品中特定细胞类型的新颖方法和组合物，并定义了每单位体积样品中这些细胞的浓度或“绝对计数”。 更具体地，本发明涉及定量抗原特异性T细胞或限定未裂解全血中所述细胞的相对百分比的方法。 此外，本发明涉及用于制备用于高速定量特定细胞类型的全血样品的试剂盒，例如， 抗原特异性T细胞，通过流式细胞仪检测所有全血样品。