公开(公告)号：US4681931A

公开(公告)日：1987-07-21

申请号：US741865

申请日：1985-06-06

申请人： Rainer Obermeier ,  Ingeborg Salomon ,  Jurgen Ludwig

发明人： Rainer Obermeier ,  Ingeborg Salomon ,  Jurgen Ludwig

IPC分类号： C12N15/09 ,  A61K38/21 ,  C07K1/22 ,  C07K14/52 ,  C07K14/555 ,  C07K14/56 ,  C07K16/00 ,  C12P21/00 ,  C07K15/26 ,  A61K45/02

CPC分类号： C07K14/56

摘要： The invention relates to a process for the isolation and purification of .alpha.-interferons generated by plasmids from bacterial cultures which have been modified by genetic engineering, and of .alpha.-interferons from culture supernatants of induced mammalian cells, which comprises partition of appropriate crude substances containing interferon between an aqueous urea solution, which contains a surfactant, and a mixture of n-butanol and glacial acetic acid which contains water, and isolation of the purified .alpha.-interferons from the upper phase.

摘要翻译： 本发明涉及用于分离和纯化由经遗传工程修饰的细菌培养物的质粒产生的α-干扰素和由诱导的哺乳动物细胞的培养物上清液产生的α-干扰素的方法，该方法包括将含有 在含有表面活性剂的尿素水溶液和含有水的正丁醇和冰醋酸的混合物之间的干扰素，以及从上部相分离纯化的α-干扰素。