公开(公告)号：US20070065881A1

公开(公告)日：2007-03-22

申请号：US10582674

申请日：2004-12-22

Applicant: Herve Perron ,  Anne Eveno-Nobile ,  Jacques Portoukalian ,  Nicole Battail-Poirot

Inventor： Herve Perron ,  Anne Eveno-Nobile ,  Jacques Portoukalian ,  Nicole Battail-Poirot

IPC: G01N33/567 ,  C07K14/705

CPC classification number: G01N33/564 ,  C07K14/47 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  C07K16/3084 ,  C07K2317/32 ,  G01N2800/285

Abstract: The invention relates to an isolated cytotoxic factor which is associated with multiple sclerosis and which is selected from the heterocomplex GM2AP/GM2/MRP14 and mutated GM2AP/GM2/MRP14, and to the method of detecting said factor in a biological sample to be tested. The inventive method comprises the following steps consisting in: (i) bringing the biological sample into contact with at least one capture antibody selected from antibodies that bind specifically to the GM2AP protein, to the mutated GM2AP protein, to the MRP14 protein, to the complex GM2AP/GM2, to the complex mutated GM2AP/GM2 and to the complex MRP14/GM2, and with at least one labeled detection antibody selected from antibodies that bind specifically to the GM2AP protein, to the mutated GM2AP protein, to the MRP14 protein, to the complex GM2AP/GM2, to the complex mutated GM2AP/GM2 and to the complex MRP14/GM2, and (ii) detecting and/or quantifying the cytotoxic factor by detecting and/or quantifying the labeled detection antibody.

Abstract translation: 本发明涉及与多发性硬化相关并且选自异型复合体GM2AP / GM2 / MRP14和突变的GM2AP / GM2 / MRP14的分离的细胞毒性因子，以及检测待测生物样品中所述因子的方法。 本发明的方法包括以下步骤：（i）将生物样品与至少一种选自与GM2AP蛋白特异性结合的抗体，至突变型GM2AP蛋白，至MRP14蛋白的捕获抗体接触至复合物 GM2AP / GM2，复合突变的GM2AP / GM2和复合物MRP14 / GM2，以及至少一种选自与GM2AP蛋白特异性结合的抗体的标记检测抗体与突变的GM2AP蛋白相关的MRP14蛋白，至 复合体GM2AP / GM2，复合突变的GM2AP / GM2和复合物MRP14 / GM2，以及（ii）通过检测和/或定量标记的检测抗体来检测和/或定量细胞毒性因子。

公开(公告)号：US07700294B2

公开(公告)日：2010-04-20

申请号：US10582674

申请日：2004-12-22

Applicant: Hervé Perron ,  Anne Eveno-Nobile ,  Jacques Portoukalian ,  Nicole Battail-Poirot

Inventor： Hervé Perron ,  Anne Eveno-Nobile ,  Jacques Portoukalian ,  Nicole Battail-Poirot

IPC: G01N33/536 ,  C07K16/18

CPC classification number: G01N33/564 ,  C07K14/47 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  C07K16/3084 ,  C07K2317/32 ,  G01N2800/285

Abstract: The invention relates to an isolated cytotoxic factor which is associated with multiple sclerosis and which is selected from the heterocomplex GM2AP/GM2/MRP14 and mutated GM2AP/GM2/MRP14, and to the method of detecting said factor in a biological sample to be tested. The inventive method comprises the following steps consisting in: (i) bringing the biological sample into contact with at least one capture antibody selected from antibodies that bind specifically to the GM2AP protein, to the mutated GM2AP protein, to the MRP14 protein, to the complex GM2AP/GM2, to the complex mutated GM2AP/GM2 and to the complex MRP14/GM2, and with at least one labeled detection antibody selected from antibodies that bind specifically to the GM2AP protein, to the mutated GM2AP protein, to the MRP14 protein, to the complex GM2AP/GM2, to the complex mutated GM2AP/GM2 and to the complex MRP14/GM2, and (ii) detecting and/or quantifying the cytotoxic factor by detecting and/or quantifying the labeled detection antibody.

Abstract translation: 本发明涉及与多发性硬化相关并且选自异型复合体GM2AP / GM2 / MRP14和突变的GM2AP / GM2 / MRP14的分离的细胞毒性因子，以及检测待测生物样品中所述因子的方法。 本发明的方法包括以下步骤：（i）将生物样品与至少一种选自与GM2AP蛋白特异性结合的抗体，至突变型GM2AP蛋白，至MRP14蛋白的捕获抗体接触至复合物 GM2AP / GM2，复合突变的GM2AP / GM2和复合物MRP14 / GM2，以及至少一种选自与GM2AP蛋白特异性结合的抗体的标记检测抗体与突变的GM2AP蛋白相关的MRP14蛋白，至 复合体GM2AP / GM2，复合突变的GM2AP / GM2和复合物MRP14 / GM2，以及（ii）通过检测和/或定量标记的检测抗体来检测和/或定量细胞毒性因子。