公开(公告)号：US5422251A

公开(公告)日：1995-06-06

申请号：US187890

申请日：1994-01-28

Applicant: Jacques R. Fresco

Inventor： Jacques R. Fresco

IPC: C12N15/113 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6839 ,  C12N15/113 ,  C12N2310/15

Abstract: A triple-stranded nucleic acid having a first nucleic acid strand that has a region of adjacent purine nucleoside residues; a second nucleic acid strand, at least a portion of which is hydrogen bonded in a Watson-Crick manner to the region of adjacent purine nucleoside residues of the first strand; and a third nucleic acid strand, at least a portion of which is hydrogen bonded to the portion of the region of adjacent purine nucleoside residues of the first strand, the portion of the region of adjacent purine nucleoside residues to which both the second strand and the third strand are bonded defining the triple-stranded nucleic acid.

Abstract translation: 具有第一核酸链的三链核酸，其具有相邻嘌呤核苷残基的区域; 第二核酸链，其至少一部分以沃森 - 克里克方式氢键合到第一链的相邻嘌呤核苷残基的区域; 和第三核酸链，其至少一部分氢键连接到第一链的相邻嘌呤核苷残基区域的部分，相邻嘌呤核苷残基区域的第二链和第二链的两部分 第三链被定义为三链核酸。

公开(公告)号：US06783932B2

公开(公告)日：2004-08-31

申请号：US09341079

申请日：1999-07-02

Applicant: Jacques R. Fresco ,  John Laurence Richard Lavelle

Inventor： Jacques R. Fresco ,  John Laurence Richard Lavelle

IPC: C12Q168

CPC classification number: C12Q1/6839 ,  C12Q2527/125

Abstract: The stability of a triplex in solution is enhanced by adding to the solution, either before or after formation of the triplex, an effective amount of either of the following: (a) a water structure-making substance, at an appropriately high concentration, other than an alkali or alkaline earth metal cation, a tetramethylammonium cation, or a polyamine; or (b) a combination of said water structure-making substance and an alkali or alkaline earth cation or a tetramethylammonium cation or a polyamine.

Abstract translation: 在溶液形成之前或之后，通过向溶液中加入有效量的以下任一种来增强溶液中三链体的稳定性：（a）适当高浓度的水结构物质，其它 比碱金属或碱土金属阳离子，四甲基铵阳离子或多胺; 或（b）所述水结构物质与碱金属或碱土金属阳离子或四甲基铵阳离子或多胺的组合。

公开(公告)号：US06426407B1

公开(公告)日：2002-07-30

申请号：US08473888

申请日：1995-06-07

Applicant: Jacques R. Fresco ,  Bin Liu ,  Lynn C. Klotz

Inventor： Jacques R. Fresco ,  Bin Liu ,  Lynn C. Klotz

IPC: C07H2102

CPC classification number: C07H21/00 ,  C12Q1/6839

Abstract: The invention relates to compositions of matter capable of serving as residues for specific binding of third strands to double-stranded complementary nucleic acids of any base-pair sequence.

Abstract translation: 本发明涉及能够作为第三链与任何碱基对序列的双链互补核酸特异性结合的残基的物质组合物。

公开(公告)号：US5693471A

公开(公告)日：1997-12-02

申请号：US459645

申请日：1995-06-02

Applicant: Jacques R. Fresco

Inventor： Jacques R. Fresco

IPC: C12N15/113 ,  C12Q1/68 ,  C07H21/04 ,  C12Q1/70 ,  C19P19/34

CPC classification number: C12Q1/6839 ,  C12N15/113 ,  C12N2310/15

Abstract: A triple-stranded nucleic acid having a first nucleic acid strand that has a region of adjacent purine nucleoside residues; a second nucleic acid strand, at least a portion of which is hydrogen bonded in a Watson-Crick manner to the region of adjacent purine nucleoside residues of the first strand; and a third nucleic acid strand, at least a portion of which is hydrogen bonded to the portion of the region of adjacent purine nucleoside residues of the first strand, the portion of the region of adjacent purine nucleoside residues to which both the second strand and the third strand are bonded defining the triple-stranded nucleic acid is disclosed.

Abstract translation: 具有第一核酸链的三链核酸，其具有相邻嘌呤核苷残基的区域; 第二核酸链，其至少一部分以沃森 - 克里克方式氢键合到第一链的相邻嘌呤核苷残基的区域; 和第三核酸链，其至少一部分氢键连接到第一链的相邻嘌呤核苷残基区域的部分，相邻嘌呤核苷残基区域的第二链和第二链的两部分 公开了限定三链核酸的第三链。

公开(公告)号：US5928863A

公开(公告)日：1999-07-27

申请号：US841218

申请日：1992-02-27

Applicant: Jacques R. Fresco

Inventor： Jacques R. Fresco

IPC: C12N15/113 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6839 ,  C12N15/113 ,  C12N2310/15

Abstract: A triple-stranded nucleic acid having a first nucleic acid strand that has a region of adjacent purine nucleoside residues; a second nucleic acid strand, at least a portion of which is hydrogen bonded in a Watson-Crick manner to the region of adjacent purine nucleoside residues of the first strand; and a third nucleic acid strand, at least a portion of which is hydrogen bonded to the portion of the region of adjacent purine nucleoside residues of the first strand, the portion of the region of adjacent purine nucleoside residues to which both the second strand and the third strand are bonded defining the triple-stranded nucleic acid.

公开(公告)号：US06461810B1

公开(公告)日：2002-10-08

申请号：US09531000

申请日：2000-09-08

Applicant: Jacques R. Fresco ,  Marion D. Johnson

Inventor： Jacques R. Fresco ,  Marion D. Johnson

IPC: C12Q170

CPC classification number: C12Q1/6883 ,  C12Q1/6839 ,  C12Q1/6841 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2537/119

Abstract: Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occured.

Abstract translation: 公开了用于原位检测基本上双链核酸片段中靶序列的存在的方法，其包括：a）在适合于将基本上双链核酸片段与可检测的第三链杂交的条件下原位接触， 如果存在所述靶序列，则所述第三链能够与目标序列的至少一部分杂交以形成三链结构; 和b）检测是否发生了第三链和靶序列之间的杂交。