公开(公告)号：US20060252152A1

公开(公告)日：2006-11-09

申请号：US10571193

申请日：2004-10-05

申请人： James Alstine ,  Hans Berg ,  Asa Bjurling

发明人： James Alstine ,  Hans Berg ,  Asa Bjurling

IPC分类号： C12N1/20 ,  C12N5/02 ,  C12N5/00 ,  C12M3/00

CPC分类号： C07K17/02 ,  C12N5/0075 ,  C12N2533/30 ,  C12N2533/70

摘要： The present invention relates to a microcarrier onto the surface of which a cationic compound has been immobilised via a guanidine group. The microcarrier is capable of attachment of cells, e.g. via charge-based interaction, and is advantageously used as a support in the culture of cells. Said compound may comprise one or two amino acids, such as arginine (Arg) or a dipeptide. The invention also relates to a method of preparing a polycationic microcarrier, which method comprises to immobilise a compound that comprises at least one guanidine group to an epoxide-activated substrate.

摘要翻译： 本发明涉及通过胍基固定有阳离子化合物的表面上的微载体。 微载体能够附着细胞，例如细胞。 通过基于电荷的相互作用，并且有利地用作细胞培养物中的载体。 所述化合物可以包含一个或两个氨基酸，例如精氨酸（Arg）或二肽。 本发明还涉及一种制备聚阳离子微载体的方法，该方法包括将包含至少一个胍基的化合物固定在环氧化物活化的底物上。